IgM Antibody (IM260)

Western Blot: IgM Antibody (IM260) [NBP2-34253] - Western Blot Analysis of Raji cell lysate using IgM Antibody (IM260)

Immunohistochemistry-Paraffin: IgM Antibody (IM260) [NBP2-34253] - Formalin-fixed, paraffin-embedded human tonsil stained with IgM Antibody (IM260)

Immunohistochemistry-Paraffin: IgM Antibody (IM260) [NBP2-34253] - Formalin-fixed, paraffin-embedded human tonsil stained with IgM MAb (IM260)

Immunohistochemistry-Paraffin: IgM Antibody (IM260) [NBP2-34253] - Human lymph node. IgM antibody at 1:200 at RT for 1 hr. IHC-P image submitted by a verified customer review.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clone: IM260
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

IgM Antibody (IM260) Summary

• Immunogen: Recombinant heavy chain of human IgM
• Localization: Cytoplasm, Cell Surface and Secreted
• Marker: B-Cell Marker
• Specificity: Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGHM
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
• Application Notes: ELISA (for coating, order NBP2-34649, the BSA free version of NBP2-34253). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for IgM Antibody (IM260)

• AGM1
• DKFZp686I15196
• DKFZp686I15212
• FLJ00385
• IgM
• immunoglobulin heavy constant mu
• MGC104996
• MGC52291
• MU
• VH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgM Antibody (NBP2-34253)(1)

Publication using NBP2-34253

• Balkhi MY, Willette-Brown J, Wittmann G, Hu Y. IKKa deficiency disrupts the development of marginal zone and follicular B cells Genes Immun. May 8 2018 [PMID: 29740197] (Human)

Review for IgM Antibody (NBP2-34253) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous IHC-P Human 09/17/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human lymph node
• Species: Human
• Lot: 3507-3P170719

Comments

• Comments: 1:200 @ room temperature for 1 hour

Bioinformatics

• Gene Symbol: IGHM
• Uniprot:
  • P01871(Human)