IgM Antibody (IM260)

Images

 
Western Blot: IgM Antibody (IM260) [NBP2-34253] - Western Blot Analysis of Raji cell lysate using IgM Antibody (IM260)
Immunohistochemistry-Paraffin: IgM Antibody (IM260) [NBP2-34253] - Formalin-fixed, paraffin-embedded human tonsil stained with IgM Antibody (IM260)
Immunohistochemistry-Paraffin: IgM Antibody (IM260) [NBP2-34253] - Formalin-fixed, paraffin-embedded human tonsil stained with IgM MAb (IM260)
Immunohistochemistry-Paraffin: IgM Antibody (IM260) [NBP2-34253] - Human lymph node. IgM antibody at 1:200 at RT for 1 hr. IHC-P image submitted by a verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
IM260
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

IgM Antibody (IM260) Summary

Immunogen
Recombinant heavy chain of human IgM
Localization
Cytoplasm, Cell Surface and Secreted
Marker
B-Cell Marker
Specificity
Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IGHM
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
Application Notes
ELISA (for coating, order NBP2-34649, the BSA free version of NBP2-34253). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-34253 in the following applications:

Publications
Read Publication using NBP2-34253.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for IgM Antibody (IM260)

  • AGM1
  • DKFZp686I15196
  • DKFZp686I15212
  • FLJ00385
  • IgM
  • immunoglobulin heavy constant mu
  • MGC104996
  • MGC52291
  • MU
  • VH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgM Antibody (NBP2-34253)(1)

We have publications tested in 1 confirmed species: Human.


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(1)
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Review for IgM Antibody (NBP2-34253) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-34253:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin IgM NBP2-34253
Enlarge
5
reviewed by:
Anonymous
IHC-P Human 09/17/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman lymph node
SpeciesHuman
Lot3507-3P170719

Comments

Comments1:200 @ room temperature for 1 hour

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgM Antibody (NBP2-34253) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34649AF350
Alexa Fluor 405 NBP2-34649AF405
Alexa Fluor 488 NBP2-34649AF488
Alexa Fluor 532 NBP2-34649AF532
Alexa Fluor 594 NBP2-34649AF594
Alexa Fluor 647 NBP2-34649AF647
Alexa Fluor 700 NBP2-34649AF700
Alexa Fluor 750 NBP2-34649AF750
Allophycocyanin NBP2-34649APC
Allophycocyanin/Cy7 NBP2-34649APCCY7
Biotin NBP2-34649B
DyLight 350 NBP2-34649UV
DyLight 405 NBP2-34649V
DyLight 488 NBP2-34649G
DyLight 550 NBP2-34649R
DyLight 594 NBP2-34649DL594
DyLight 650 NBP2-34649C
DyLight 680 NBP2-34649FR
DyLight 755 NBP2-34649IR
FITC NBP2-34649F
HRP NBP2-34649H
Janelia Fluor 549 NBP2-34649JF549
Janelia Fluor 646 NBP2-34649JF646
PE NBP2-34649PE
PE/Atto594 NBP2-34649PEATT594
PE/Cy5.5 NBP2-34649PECY55
PE/Cy7 NBP2-34649PECY7

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Bioinformatics Tool for IgM Antibody (NBP2-34253)

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Blogs on IgM.

How to identify B cell subsets using flow cytometry
By Victoria OsinskiUsing flow cytometry to identify B cell subsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified a...  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
09/17/2019
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol IGHM
Uniprot