Rabbit IgG ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity RbSpecies Glossary
Applications ELISA
Suitable Sample Type
serum, plasma
Standard Curve Range
0.047 ug/ml-12 ug/ml
Sensitivity
0.029 ug/ml

Order Details

Rabbit IgG ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of rabbit IgG. No significant cross-reactivity or interference between rabbit IgG and analogues was observed.
Standard Curve Range
0.047 ug/ml-12 ug/ml
Sensitivity
0.029 ug/ml
Assay Type
Competitive ELISA
Inter-Assay
CV%<10%
Intra-Assay
CV%<8%
Sample Volume
50-100ul
Kit Type
ELISA Kit (Colorimetric)

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.

Kit Components

Components
  1. HRP-conjugate
  2. Instruction manual
  3. Assay plate
  4. Adhesive Strip (For 96 wells)
  5. Stop Solution
  6. Standard
  7. Sample Diluent 2 x 20 ml
  8. TMB Substrate
  9. Wash Buffer (25 x concentrate)

Notes

Please contact our Technical Services with any questions regarding species reactivity

Alternate Names for Rabbit IgG ELISA Kit (Colorimetric)

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Background

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with rabbit IgG. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for rabbit IgG. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of rabbit IgG in samples. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Research Areas for IgG ELISA Kit (NBP2-60063)

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Blogs on IgG.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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