Rat IgG ELISA Kit (Colorimetric)

• Reactivity: Rt
• Applications: ELISA, NULL
• Suitable Sample Type: Biological Samples, Plasma, Serum
• Standard Curve Range: 25 to 800 ng/mL
• Sensitivity: 2.894

Rat IgG ELISA Kit (Colorimetric) Summary

• Description: IgG (Rat) ELISA Kit is a highly sensitive two-site enzyme-linked immunoassay for the quantitative determination of IgG in rat biological samples.
• Standard Curve Range: 25 to 800 ng/mL
• Sensitivity: 2.894
• Assay Type: Two-site enzyme-linked immunoassay
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IGHG1

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rat IgG ELISA Kit (Colorimetric)

• IgG
• Immunoglobulin G
• ImmunoglobulinG

Background

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for IgG ELISA Kit (KA1952)(3)

Publications using KA1952

• Iria T, Marta V, Tatjana S et al. Prediction of subcutaneous drug absorption - Characterization of subcutaneous interstitial fluids as a basis for developing biorelevant in vitro models. Int J Pharm. 2023-03-29 [PMID: 37001831]
• Audrey K, Leng K, Ji T et al. Clinacanthus nutans aqueous leaves extract exerts anti-allergic activity in preclinical anaphylactic models via alternative IgG pathway. J Ethnopharmacol. 2022-12-02 [PMID: 36464074]
• Sulaiman I, Tan K, Mohtarrudin N et al. Andrographolide prevented toluene diisocyanate-induced occupational asthma and aberrant airway E-cadherin distribution via p38 MAPK-dependent Nrf2 induction. Pulm Pharmacol Ther 2018-09-21 [PMID: 30244166]

FAQs for IgG ELISA Kit (KA1952). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IGHG1