Donkey anti-Mouse IgG Secondary Antibody [NL557]

Detection of human Vimentin by incubating cells with 8 μg/mL of Mouse anti-Human Vimentin monoclonal antibody (MAB21052) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A172 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

• Reactivity: Mu
• Applications: Flow, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Donkey
• Conjugate: NL557
• Suitable Sample Type: 23042

Donkey anti-Mouse IgG Secondary Antibody [NL557] Summary

• Additional Information: NorthernLights 557 Fluorochrome-labeled Antibody, X Absorbed
• Immunogen: Mouse IgG
• Specificity: Mouse IgG (H+L)
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Donkey
• Gene: IGHG1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
  *Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Mouse IgG Secondary Antibody [NL557]

• IgG
• Immunoglobulin G
• ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (NL007)(33)

Publications using NL007

• Prusek-Kucharek, A;Sikora, B;Czekaj, P; Potential Therapeutic Effects of Epithelial and Mesenchymal Stem Cell Secretome in Benzalkonium Chloride-Induced Limbal Stem Cell Dysfunction Cells 2025-11-14 [PMID: 41294843] (Immunocytochemistry, Human)
• Tonin, R;Feo, F;Falliano, S;Giunti, L;Calamai, M;Procopio, E;Mari, F;Sciruicchio, V;Conti, V;Fanelli, I;Bambi, F;Guerrini, R;Morrone, A; Generation of a human induced pluripotent stem cell line from a patient with GM3 synthase deficiency using self-replicating RNA vector Stem cell research 2024-05-03 [PMID: 38703669] (Immunocytochemistry, Human)
• AA Hill, M Kim, DF Zegarra-Ru, LC Chang, KC Norwood, A Assié, WH Wu, MC Renfroe, HW Song, AM Major, BS Samuel, JM Hyser, RS Longman, GE Diehl Acute high fat diet impairs macrophage supported intestinal damage resolution JCI Insight, 2023-02-08;0(0):. 2023-02-08 [PMID: 36538527] (IHC, Mouse)
• Maya Rozenfeld, Ivana Savic Azoulay, Tsipi Ben Kasus Ben Kasus Nissim, Alexandra Stavsky, Moran Melamed, Grace Stutzmann et al. Essential role of the mitochondrial Na+/Ca2+ exchanger NCLX in mediating PDE2-dependent neuronal survival and learning Cell Reports 2022-12-06 [PMID: 36476859] (Immunocytochemistry, Mouse, Transgenic Mouse)
• Maya Rozenfeld, Ivana Savic Azoulay, Tsipi Ben Kasus Ben Kasus Nissim, Alexandra Stavsky, Moran Melamed, Grace Stutzmann et al. Essential role of the mitochondrial Na+/Ca2+ exchanger NCLX in mediating PDE2-dependent neuronal survival and learning Cell Reports 2022-12-06 [PMID: 36476859]
• H Lin, SQ Ye, ZH Xu, JS Penaloza, M Aljuhani, T Vetter, MT Zhao, KL McBride Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD) Stem Cell Research, 2022-10-31;65(0):102958. 2022-10-31 [PMID: 36343514] (ICC, Human)
• István Fodor, Tamar Schwarz, Bence Kiss, Antal Tapodi, János Schmidt, Alex R. O. Cousins et al. Studies on a widely-recognized snail model species (Lymnaea stagnalis) provide further evidence that vertebrate steroids do not have a hormonal role in the reproduction of mollusks Front Endocrinol (Lausanne) 2022-09-08 [PMID: 36157463]
• Jinrong Peng, Yao Xiao, Qian Yang, Qingya Liu, Yu Chen, Kun Shi et al. Intracellular aggregation of peptide-reprogrammed small molecule nanoassemblies enhances cancer chemotherapy and combinatorial immunotherapy Acta Pharmaceutica Sinica B 2021-04-01 [PMID: 33996418]
• P Mergenthal, S Hariharan, JM Pemberton, C Lourenco, LZ Penn, DW Andrews Rapid 3D phenotypic analysis of neurons and organoids using data-driven cell segmentation-free machine learning PLoS computational biology, 2021-02-22;17(2):e1008630. 2021-02-22 [PMID: 33617523] (ICC, Human)
• LA Alves Avel, C Schrenk, M Sönnichsen, A Hamacher, FK Hansen, J Schliehe-D, A Borkhardt, S Bhatia, MU Kassack, T Kurz Synergistic induction of apoptosis in resistant head and neck carcinoma and leukemia by alkoxyamide-based histone deacetylase inhibitors European Journal of Medicinal Chemistry, 2020-12-17;211(0):113095. 2020-12-17 [PMID: 33360560] (Cell Culture, Human)
• G Kaufman, D Skrtic N-Acetyl Cysteine Modulates the Inflammatory and Oxidative Stress Responses of Rescued Growth-Arrested Dental Pulp Microtissues Exposed to TEGDMA in ECM Int J Mol Sci, 2020-10-03;21(19):. 2020-10-03 [PMID: 33023018] (ICC, Human)
• Dhananjay V. Deshmukh, Nils Pasquero, Gajraj Rathore, Joel Zvick, Ori Bar‐Nur, Jurg Dual et al. Screening method to identify hydrogel formulations that facilitate myotube formation from encapsulated primary myoblasts Bioengineering & Translational Medicine 2020-09-03 [PMID: 33005743]
• C Seraya-Bar, A Weisz, E Shinderman, S Teper-Roth, D Stamler, N Arbib, Y Kadan, A Fishman, D Kidron, E Edelstein, M Ellis, O Ashur-Fabi The identification of nuclear &amp;alphav&amp;beta3 integrin in ovarian cancer: non-paradigmal localization with cancer promoting actions Oncogenesis, 2020-07-29;9(7):69. 2020-07-29 [PMID: 32728020] (ICC, Human)
• M Li, D Xu Antiresorptive activity of osteoprotegerin requires an intact heparan sulfate-binding site Proc. Natl. Acad. Sci. U.S.A., 2020-07-07;0(0):. 2020-07-07 [PMID: 32636266] (ICC, Mouse)
• X Hou, G Chen, W Bracamonte, HS Choi, NL Diny, J Sung, D Hughes, T Won, MK Wood, MV Talor, DJ Hackam, K Klingel, G Davogustto, H Taegtmeyer, I Coppens, JG Barin, D ?iháková The Cardiac Microenvironment Instructs Divergent Monocyte Fates and Functions in Myocarditis Cell Rep, 2019-07-02;28(1):172-189.e7. 2019-07-02 [PMID: 31269438] (IHC, Human)
• T Liu, F Gonzalez D, Y Zhao, Z Wu, AE Rinke, KK Kim, SH Phan Telomerase reverse transcriptase ameliorates lung fibrosis by protecting alveolar epithelial cells against senescence J. Biol. Chem., 2019-04-18;0(0):. 2019-04-18 [PMID: 31000627] (IHC-P, Human)
• S Kany, JP Horstmann, R Sturm, K Mörs, B Relja Reduced NLRP3 Gene Expression Limits the IL-1? Cleavage via Inflammasome in Monocytes from Severely Injured Trauma Patients Mediators Inflamm., 2018-05-09;2018(0):1752836. 2018-05-09 [PMID: 29861655] (ICC, Human)
• MH Amer, FRAJ Rose, KM Shakesheff, LJ White A biomaterials approach to influence stem cell fate in injectable cell-based therapies Stem Cell Res Ther, 2018-02-21;9(1):39. 2018-02-21 [PMID: 29467014]
• Yana Dautova, Alexander N. Kapustin, Kevin Pappert, Matthias Epple, Hanneke Okkenhaug, Simon J. Cook et al. Calcium phosphate particles stimulate interleukin-1 beta release from human vascular smooth muscle cells: A role for spleen tyrosine kinase and exosome release Journal of Molecular and Cellular Cardiology 2018-02-01 [PMID: 29274344]
• X Han, X Dong, J Li, M Wang, L Luo, Z Li, X Lu, R He, R Xu, M Gong Free paclitaxel-loaded E-selectin binding peptide modified micelle self-assembled from hyaluronic acid-paclitaxel conjugate inhibit breast cancer metastasis in a murine model Int J Pharm, 2017-05-31;528(2):33-46. 2017-05-31 [PMID: 28576551] (ICC, Human)
• Michiko Ishikawa Antithrombin III improved neutrophil extracellular traps in lung after the onset�of�endotoxemia J. Surg. Res, 2016-09-28;208(0):140-150. 2016-09-28 [PMID: 27993201] (IHC-P, Mouse)
• Pengcui Li, Jin Deng, Xiaochun Wei, Chathuraka T. Jayasuriya, Jingming Zhou, Qian Chen et al. Blockade of hypoxia-induced CXCR4 with AMD3100 inhibits production of OA-associated catabolic mediators IL-1 beta and MMP-13 Molecular Medicine Reports 2016-08-01 [PMID: 27356492] (Immunocytochemistry, Human)
• Pornthida Poosala, Hirofumi Ichinose, Takuya Kitaoka Spatial Geometries of Self-Assembled Chitohexaose Monolayers Regulate Myoblast Fusion International Journal of Molecular Sciences 2016-05-06 [PMID: 27164094]
• Sauder C, Koziel J, Choi M, Fox M, Grimes B, Badve S, Blosser R, Radovich M, Lam C, Vaughan M, Herbert B, Clare S Phenotypic plasticity in normal breast derived epithelial cells. BMC Cell Biol, 2014-06-10;15(0):20. 2014-06-10 [PMID: 24915897] (ICC, Human)
• Anping Cai, Ruofeng Qiu, Liwen Li, Dongdan Zheng, Yugang Dong, Danqing Yu et al. Atorvastatin Treatment of Rats with Ischemia-Reperfusion Injury Improves Adipose-Derived Mesenchymal Stem Cell Migration and Survival via the SDF-1 alpha /CXCR-4 Axis PLoS ONE 2013-12-02 [PMID: 24312447]
• Primitive stem cells derived from bone marrow express glial and neuronal markers and support revascularization in injured retina exposed to ischemic and mechanical damage. Stem Cells Dev., 2011-10-24;21(9):1488-500. 2011-10-24 [PMID: 21905921] (IHC-Fr, Mouse)
• Gopalakrishna-Pillai S, Iverson LE A DNMT3B Alternatively Spliced Exon and Encoded Peptide Are Novel Biomarkers of Human Pluripotent Stem Cells. PLoS ONE, 2011-06-15;6(6):e20663. 2011-06-15 [PMID: 21698279] (ICC, Human)
• Mentrup B, Marschall C, Barvencik F, Amling M, Plendl H, Jakob F, Beck C Functional characterization of a novel mutation localized in the start codon of the tissue-nonspecific alkaline phosphatase gene. Bone, 2011-03-16;48(6):1401-8. 2011-03-16 [PMID: 21419245] (ICC, Human)
• Al-Lamki RS, Sadler TJ, Wang J Tumor necrosis factor receptor expression and signaling in renal cell carcinoma. Am. J. Pathol., 2010-06-21;177(2):943-54. 2010-06-21 [PMID: 20566746] (ICC, Human)
• Wessels JT, Busse AC, Mahrt J, Hoffschulte B, Mueller GA, Tarnok A, Mittag A NorthernLights in slide-based cytometry and microscopy. Cytometry A, 2010-05-01;77(5):420-8. 2010-05-01 [PMID: 20151452] (IHC, Human)
• Kubilus JK, Linsenmayer TF Developmental corneal innervation: interactions between nerves and specialized apical corneal epithelial cells. Invest. Ophthalmol. Vis. Sci., 2009-09-09;51(2):782-9. 2009-09-09 [PMID: 19741242] (IHC-Fr, Chicken)
• Moulakakis C, Stamme C Role of clathrin-mediated endocytosis of surfactant protein A by alveolar macrophages in intracellular signaling. Am. J. Physiol. Lung Cell Mol. Physiol., 2009-01-09;296(3):L430-41. 2009-01-09 [PMID: 19136579] (ICC, Rat)
• Hollins F, Kaur D, Yang W, Cruse G, Saunders R, Sutcliffe A, Berger P, Ito A, Brightling CE, Bradding P Human airway smooth muscle promotes human lung mast cell survival, proliferation, and constitutive activation: cooperative roles for CADM1, stem cell factor, and IL-6. J. Immunol., 2008-08-15;181(4):2772-80. 2008-08-15 [PMID: 18684968] (ICC, Human)

Bioinformatics

• Gene Symbol: IGHG1