Chicken anti-Goat IgG Secondary Antibody [HRP]

Western blot shows detection of Goat Anti-Human/Mouse/Rat Carboxylesterase 1/CES1 Polyclonal Antibody (AF4920) using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Vimentin in human kidney using 5 ug/mL of Goat anti-human Vimentin primary antibody (AF2105) followed by 30 min incubation with Donkey Anti-Goat HRP conjugated secondary antibodies (Catalog# HAF019) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.

• Reactivity: Gt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: HRP

Chicken anti-Goat IgG Secondary Antibody [HRP] Summary

• Immunogen: Goat IgG
• Specificity: Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross‑reactivity with mouse IgG, rabbit IgG and human IgG is observed.
• Source: N/A
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: IGHG1
• Purity Statement: Antigen Affinity-purified from egg yolks

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied as a 0.2 μm filtered solution in Sodium Phosphate and Proclin 300.
• Preservative: No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chicken anti-Goat IgG Secondary Antibody [HRP]

• IgG
• Immunoglobulin G
• ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (HAF019)(42)

Publications using HAF019

• Lai, SW;Chang, CY;Lee, HJ;Chen, YC; Identification of two point mutations associated with inherited antithrombin deficiency Thrombosis journal 2024-12-03 [PMID: 39627773] (Western Blot, Human)
• Rajwani, J;Vishnevskiy, D;Turk, M;Naumenko, V;Gafuik, C;Kim, DS;Mah, LK;Snelling, S;Gonzales, GA;Xue, J;Chanda, A;Potts, KG;Todesco, HM;Lau, KCK;Hildebrand, KM;Chan, JA;Liao, S;Monument, MJ;Hyrcza, M;Bose, P;Jenne, CN;Canton, J;Zemp, FJ;Mahoney, DJ; VSV?M51 drives CD8+ T cell-mediated tumour regression through infection of both cancer and non-cancer cells Nature communications 2024-11-15 [PMID: 39548070] (Western Blot, Mouse)
• Kosmicki, JA;Marcketta, A;Sharma, D;Di Gioia, SA;Batista, S;Yang, XM;Tzoneva, G;Martinez, H;Sidore, C;Kessler, MD;Horowitz, JE;Roberts, GHL;Justice, AE;Banerjee, N;Coignet, MV;Leader, JB;Park, DS;Lanche, R;Maxwell, E;Knight, SC;Bai, X;Guturu, H;Baltzell, A;Girshick, AR;McCurdy, SR;Partha, R;Mansfield, AJ;Turissini, DA;Zhang, M;Mbatchou, J;Watanabe, K;Verma, A;Sirugo, G;Colorado Center for Precision Medicine, ;Mayo Clinic Project Generation, ;Regeneron Genetics Center, ;University of California Los Angeles ATLAS Collaboration, ;Ritchie, MD;Salerno, WJ;Shuldiner, AR;Rader, DJ;Mirshahi, T;Marchini, J;Overton, JD;Carey, DJ;Habegger, L;Reid, JG;Economides, A;Kyratsous, C;Karalis, K;Baum, A;Cantor, MN;Rand, KA;Hong, EL;Ball, CA;Siminovitch, K;Baras, A;Abecasis, GR;Ferreira, MAR; Genetic risk factors for COVID-19 and influenza are largely distinct Nature genetics 2024-08-05 [PMID: 39103650] (Western Blot, Human)
• Abello, J;Yin, Y;Zhao, Y;Maurer, J;Lee, J;Bodell, C;Clevenger, AJ;Burton, Z;Goeckel, ME;Lin, M;Grainger, S;Halabi, CM;Raghavan, SA;Sah, R;Stratman, AN; Endothelial cell Piezo1 promotes vascular smooth muscle cell differentiation on large arteries bioRxiv : the preprint server for biology 2024-06-12 [PMID: 38915529] (Western Blot, Human)
• Huang, P;Åbacka, H;Wilson, CJ;Wind, ML;R?tzler, M;Hagström-Andersson, A;Gourdon, P;de Groot, BL;Venskutonyt?, R;Lindkvist-Petersson, K; Molecular basis for human aquaporin inhibition Proceedings of the National Academy of Sciences of the United States of America 2024-02-13 [PMID: 38319972] (Western Blot, Human)
• Su, H;Guo, H;Qiu, X;Lin, TY;Qin, C;Celio, G;Yong, P;Senders, M;Han, X;Bernlohr, DA;Chen, X; Lipocalin 2 regulates mitochondrial phospholipidome remodeling, dynamics, and function in brown adipose tissue in male mice Nature communications 2023-10-23 [PMID: 37872178] (Bioassay, Mouse)
• Zuo, Y;Zhang, C;Zhou, Y;Li, H;Xiao, W;Herzog, RW;Xu, J;Zhang, J;Chen, YE;Han, R; Liver-specific in vivo base editing of Angptl3 via AAV delivery efficiently lowers blood lipid levels in mice Cell & bioscience 2023-06-15 [PMID: 37322547] (Western Blot, Mouse)
• Xu, AF;Molinuevo, R;Fazzari, E;Tom, H;Zhang, Z;Menendez, J;Casey, KM;Ruggero, D;Hinck, L;Pritchard, JK;Barna, M; Subfunctionalized expression drives evolutionary retention of ribosomal protein paralogs Rps27 and Rps27l in vertebrates eLife 2023-06-12 [PMID: 37306301] (Mouse)
• Sheng Wang, Maohua Huang, Minfeng Chen, Zhiting Sun, Yubo Jiao, Geni Ye et al. Zoledronic acid and thymosin alpha 1 elicit antitumor immunity against prostate cancer by enhancing tumor inflammation and cytotoxic T cells Journal for ImmunoTherapy of Cancer 6/9/2023 [PMID: 37295817]
• E Sucajtys-S, A Debska-Sli, B Rutkowski, R Milczarek, M Szolkiewic, J Swierczyns, RT Smolenski Hepatocyte Nuclear Factor-1alpha Increases Fibrinogen Gene Expression in Liver and Plasma Fibrinogen Concentration in Rats with Experimental Chronic Renal Failure International Journal of Molecular Sciences, 2023-03-17;24(6):. 2023-03-17 [PMID: 36982805] (Western Blot, Rat)
• D Langgartne, M Amoroso, E Kempter, M Kustermann, J Scheurer, CA Lowry, G Strau beta , SO Reber Mycobacterium vaccae protects against glucocorticoid resistance resulting from combined physical and psychosocial trauma in mice Brain, Behavior, and Immunity, 2023-02-01;109(0):221-234. 2023-02-01 [PMID: 36736929] (Western Blot, Mouse)
• Marcella Birtele, Petter Storm, Yogita Sharma, Janko Kajtez, Jenny Nelander Wahlestedt, Edoardo Sozzi et al. Single-cell transcriptional and functional analysis of dopaminergic neurons in organoid-like cultures derived from human fetal midbrain Development 12/1/2022 [PMID: 36305490]
• E Sucajtys-S, A Debska-Sli, B Rutkowski, M Szolkiewic, J Swierczyns, RT Smolenski Hepatocyte Nuclear Factor 1alpha Proinflammatory Effect Linked to the Overexpression of Liver Nuclear Factor-kappaB in Experimental Model of Chronic Kidney Disease International Journal of Molecular Sciences, 2022-08-10;23(16):. 2022-08-10 [PMID: 36012158] (Western Blot, Rat)
• L Xiong, EA Tolen, J Choi, S Velychko, L Caizzi, T Velychko, K Adachi, CM MacCarthy, M Lidschreib, P Cramer, HR Schöler Oct4 differentially regulates chromatin opening and enhancer transcription in pluripotent stem cells Elife, 2022-05-27;11(0):. 2022-05-27 [PMID: 35621159] (Western Blot, Mouse)
• Yang Ran, Zhiyuan Xu, Minfeng Chen, Wei Wang, Yang Wu, Jiexuan Cai et al. Fiber‐Optic Theranostics (FOT): Interstitial Fiber‐Optic Needles for Cancer Sensing and Therapy Advanced Science 5/1/2022 [PMID: 35319824]
• Shiv Ram Krishn, Vaughn Garcia, Nicole M. Naranjo, Fabio Quaglia, Christopher D. Shields, Maisha A. Harris et al. Small extracellular vesicle-mediated ITGB6 siRNA delivery downregulates the alpha V beta 6 integrin and inhibits adhesion and migration of recipient prostate cancer cells Cancer Biology & Therapy 12/31/2022 [PMID: 35188070]
• Fujii K, Zhulyn O, Byeon GW Et al. Controlling tissue patterning by translational regulation of signaling transcripts through the core translation factor eIF3c Developmental cell 11/8/2021 [PMID: 34752747]
• Huang K, Jo H, Echesabal-Chen J, Stamatikos A Combined LXR and RXR Agonist Therapy Increases ABCA1 Protein Expression and Enhances ApoAI-Mediated Cholesterol Efflux in Cultured Endothelial Cells Metabolites 9/18/2021 [PMID: 34564456]
• YG Gete, LW Koblan, X Mao, M Trappio, B Mahadik, JP Fisher, DR Liu, K Cao Mechanisms of angiogenic incompetence in Hutchinson-Gilford progeria via downregulation of endothelial NOS Aging Cell, 2021-06-04;0(0):e13388. 2021-06-04 [PMID: 34086398] (Western Blot, Human)
• KP Kim, C Li, D Bunina, HW Jeong, J Ghelman, J Yoon, B Shin, H Park, DW Han, JB Zaugg, J Kim, T Kuhlmann, RH Adams, KM Noh, SA Goldman, HR Schöler Donor cell memory confers a metastable state of directly converted cells Cell Stem Cell, 2021-04-12;0(0):. 2021-04-12 [PMID: 33848472] (Flow Cytometry, Mouse)
• Y Wang, Y Mi, J Tian, X Qiao, X Su, J Kang, Z Wu, G Wang, X Zhou, Y Zhou, R Li Intermedin Alleviates Renal Ischemia-Reperfusion Injury and Enhances Neovascularization in Wistar Rats Drug Des Devel Ther, 2020-11-10;14(0):4825-4834. 2020-11-10 [PMID: 33204068] (IHC, Rat)
• KP Kim, Y Wu, J Yoon, K Adachi, G Wu, S Velychko, CM MacCarthy, B Shin, A Röpke, MJ Arauzo-Bra, M Stehling, DW Han, Y Gao, J Kim, S Gao, HR Schöler Reprogramming competence of OCT factors is determined by transactivation domains Science Advances, 2020-09-02;6(36):. 2020-09-02 [PMID: 32917606] (Western Blot, Mouse)
• S Zalba, AM Contreras-, E Martisova, R Debets, C Smerdou, MJ Garrido Quantification of Pharmacokinetic Profiles of PD-1/PD-L1 Antibodies by Validated ELISAs Pharmaceutics, 2020-06-26;12(6):. 2020-06-26 [PMID: 32604843] (ELISA Detection, Mouse)
• L Ou, X He, N Liu, Y Song, J Li, L Gao, X Huang, Z Deng, X Wang, S Lin Sialylation of FGFR1 by ST6Gal?I overexpression contributes to ovarian cancer cell migration and chemoresistance Mol Med Rep, 2020-01-20;21(3):1449-1460. 2020-01-20 [PMID: 32016470] (Western Blot, Human)
• Nyunt T, Britton M, Wanichthanarak K et al. Mitochondrial oxidative stress-induced transcript variants of ATF3 mediate lipotoxic brain microvascular injury Free Radic. Biol. Med. 7/26/2019 [PMID: 31356870]
• Jianjun Wang, Yongliang Yao, Qinghui Zhang, Shasha Li, Lijun Tang Inflammatory responses induced by Helicobacter pylori on the carcinogenesis of gastric epithelial GES‑1 cells International Journal of Oncology 4/9/2019 [PMID: 31081048]
• SJ Thygesen, KE Takizawa, AAB Robertson, DP Sester, KJ Stacey Compromised NLRP3 and AIM2 inflammasome function in autoimmune NZB/W F1 mouse macrophages Immunol. Cell Biol., 2018-08-19;0(0):. 8/19/2018 [PMID: 30052286]
• X Miao, MF Rahman, L Jiang, Y Min, S Tan, H Xie, L Lee, M Wang, RE Malmström, WO Lui, N Li Thrombin-reduced miR-27b attenuates platelet angiogenic activities in vitro via enhancing platelet synthesis of anti-angiogenic thrombospondin-1 J. Thromb. Haemost., 2018-03-15;0(0):. 2018-03-15 [PMID: 29442415] (Western Blot, Human)
• F Navid, G Layh-Schmi, KA Sikora, A Cougnoux, RA Colbert The Role of Autophagy in the Degradation of Misfolded HLA-B27 Heavy Chains Arthritis & rheumatology (Hoboken, N.J.), 2018-03-25;0(0):. 2018-03-25 [PMID: 29342507] (Western Blot, Rat)
• Elzbieta Sucajtys-Szulc, Alicja Debska-Slizien, Boleslaw Rutkowski, Ryszard Milczarek, Iwona Pelikant-Malecka, Tomasz Sledzinski et al. Hepatocyte nuclear factors as possible C-reactive protein transcriptional inducer in the liver and white adipose tissue of rats with experimental chronic renal failure Molecular and Cellular Biochemistry 9/1/2018 [PMID: 29330688]
• XP Zhang, ZJ Hu, AH Meng, GC Duan, QT Zhao, J Yang Role of CCL20/CCR6 and the ERK signaling pathway in lung adenocarcinoma Oncol Lett, 2017-10-23;14(6):8183-8189. 2017-10-23 [PMID: 29250193] (IHC-P, Human)
• K Tang, C Zhang, Y Zhang, Y Zhang, R Zhuang, B Jin, Y Ma Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity J Immunol Res, 2017-03-23;2017(0):6468097. 2017-03-23 [PMID: 28424792] (Western Blot, Human)
• E Casta¤o, L Caviedes, S Hirsch, M Llanos, G I¤iguez, AM Ronco Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels PLoS ONE, 2017-01-19;12(1):e0170389. 2017-01-19 [PMID: 28103309] (Western Blot, Human)
• K. Kehoe, J. F. Gielis, G. Vliegen, R. Van Elzen, R. Verkerk, E. Driessens et al. Dysregulation of the renin-angiotensin system during lung ischemia-reperfusion injury Experimental Lung Research 7/21/2016 [PMID: 27437782]
• Elzbieta Sucajtys-Szulc, Marek Szolkiewicz, Julian Swierczynski, Boleslaw Rutkowski Up-regulation of liver Pcsk9 gene expression as a possible cause of hypercholesterolemia in experimental chronic renal failure Molecular and Cellular Biochemistry 1/1/2016 [PMID: 26481479]
• Sester D, Sagulenko V, Thygesen S, Cridland J, Loi Y, Cridland S, Masters S, Genske U, Hornung V, Andoniou C, Sweet M, Degli-Esposti M, Schroder K, Stacey K Deficient NLRP3 and AIM2 Inflammasome Function in Autoimmune NZB Mice. J Immunol, 2015-06-26;195(3):1233-41. 2015-06-26 [PMID: 26116505] (Western Blot, Mouse)
• XU-DONG WANG, QIANG WANG, XIAO-LIN CHEN, JIAN-FEI HUANG, YONG YIN, PENG DA et al. Trop2 inhibition suppresses the proliferation and invasion of laryngeal carcinoma cells via the extracellular signal-regulated kinase/mitogen-activated protein kinase pathway Molecular Medicine Reports 7/1/2015 [PMID: 25779928]
• Cho JS, Guo Y, Ramos RI et al. Neutrophil-derived IL-1B is sufficient for abscess formation in immunity against Staphylococcus aureus in mice. PLoS Pathog. 1/1/2012 [PMID: 23209417]
  
  Details:
  Inhibition of S. aureus (Fig 6C) and MRSA (Fig 6D) activation in mouse neutrophils isolated from mouse bone marrow. The glibenclamide inhibitor was used at 100 micromolar. Readout assay: IL-1B production. Glibenclamide inhibited IL-1B production by about
• Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma. Biomark Insights, 2012-07-10;7(0):87-104. 2012-07-10 [PMID: 22837640] (Western Blot, Mouse)
• Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation. PLoS ONE, 2010-09-24;5(9):e12995. 2010-09-24 [PMID: 20886049] (Western Blot, Mouse)
• Kentaro Matsushima, Takashi Suyama, Chiemi Takenaka, Naoki Nishishita, Keiko Ikeda, Yoshito Ikada et al. Secreted Frizzled Related Protein 4 Reduces Fibrosis Scar Size and Ameliorates Cardiac Function After Ischemic Injury Tissue Engineering Part A 11/1/2010 [PMID: 20528676]
• Greig JA, Buckley SM, Waddington SN, Parker AL, Bhella D, Pink R, Rahim AA, Morita T, Nicklin SA, McVey JH, Baker AH Influence of coagulation factor x on in vitro and in vivo gene delivery by adenovirus (Ad) 5, Ad35, and chimeric Ad5/Ad35 vectors. Mol. Ther., 2009-07-14;17(10):1683-91. 2009-07-14 [PMID: 19603000] (Western Blot, Human)

Review for IgG Antibody (HAF019) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/02/2020

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell
• Species: Human
• Lot: WXJ0717121

Bioinformatics

• Gene Symbol: IGHG1