Goat anti-Rat IgG Secondary Antibody [HRP (Horseradish Peroxidase)]


Simple Western lane view shows detection of RatAnti-Mouse S100A9 Monoclonal Antibody (Catalog #MAB2065)using a 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was ...read more
Western blot shows detection of Rat Anti-Human/Mouse Wnt-5a MonoclonalAntibody (Catalog # MAB645)using a 1:1000 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody(Catalog # HAF005). This experiment wasconducted ...read more

Product Details

Reactivity RtSpecies Glossary
Applications WB, Simple Western
HRP (Horseradish Peroxidase)

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Goat anti-Rat IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Summary

Rat IgG
Detects rat IgG1, IgG2A, IgG2B heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with human IgG and rabbit IgG is observed.


Read Publications using
HAF005 in the following applications:

Packaging, Storage & Formulations

Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.
Proclin 300


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat anti-Rat IgG Secondary Antibody [HRP (Horseradish Peroxidase)]

  • IgG


R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (HAF005)(12)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, N/A.

We have publications tested in 3 applications: IHC-P, Neutralization, Western Blot.

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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using HAF005 Applications Species
M Leclerc, E Voilin, G Gros, S Corgnac, V de Montpré, P Validire, G Bismuth, F Mami-Choua Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1 Nat Commun, 2019;10(1):3345. 2019 [PMID: 31350404] (Western Blot, Human) Western Blot Human
B Stojanovic, I Jovanovic, BS Stojanovic, MD Stojanovic, N Gajovic, G Radosavlje, J Pantic, N Arsenijevi, ML Lukic Deletion of Galectin-3 attenuates acute pancreatitis in mice by affecting activation of innate inflammatory cells Eur. J. Immunol., 2019;0(0):. 2019 [PMID: 30892686] (IHC-P, Mouse) IHC-P Mouse
YH Lo, M Sobhany, AL Hsu, BL Ford, JM Krahn, MJ Borgnia, RE Stanley Cryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7 Nat Commun, 2019;10(1):513. 2019 [PMID: 30705282] (Western Blot) Western Blot
DT Dao, L Anez-Busti, SS Jabbouri, A Pan, H Kishikawa, PD Mitchell, GL Fell, MA Baker, RS Watnick, H Chen, MS Rogers, DR Bielenberg, M Puder A paradoxical method to enhance compensatory lung growth: Utilizing a VEGF inhibitor PLoS ONE, 2018;13(12):e0208579. 2018 [PMID: 30566445] (Western Blot, Mouse) Western Blot Mouse
L Kägi, C Bettoni, EM Pastor-Arr, U Schnitzbau, N Hernando, CA Wagner Regulation of vitamin D metabolizing enzymes in murine renal and extrarenal tissues by dietary phosphate, FGF23, and 1,25(OH)2D3 PLoS ONE, 2018;13(5):e0195427. 2018 [PMID: 29771914] (Western Blot, Mouse) Western Blot Mouse
Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation Nat. Med, 2016;0(0):. 2016 [PMID: 27941793] (Western Blot, Mouse) Western Blot Mouse
Hipp M, Shepherd D, Booth S, Waithe D, Reis E Sousa C, Cerundolo V The Processed Amino-Terminal Fragment of Human TLR7 Acts as a Chaperone To Direct Human TLR7 into Endosomes. J Immunol, 2015;194(11):5417-25. 2015 [PMID: 25917086] (Western Blot, Human) Western Blot Human
Khan A, Amu S, Saunders S, Hams E, Blackshields G, Leonard M, Weaver C, Sparwasser T, Sheils O, Fallon P Ligation of TLR7 on CD19(+) CD1d(hi) B cells suppresses allergic lung inflammation via regulatory T cells. Eur J Immunol, 2015;45(6):1842-54. 2015 [PMID: 25763771] (Neutralization, Mouse) Neutralization Mouse
MacMillan, Connor D, Leong, Hon S, Dales, David W, Robertson, Amy E, Lewis, John D, Chambers, Ann F, Tuck, Alan B Stage of breast cancer progression influences cellular response to activation of the WNT/planar cell polarity pathway. Sci Rep, 2014;4(0):6315. 2014 [PMID: 25204426] (Western Blot, Human) Western Blot Human
Spichtig D, Zhang H, Mohebbi N, Pavik I, Petzold K, Stange G, Saleh L, Edenhofer I, Segerer S, Biber J, Jaeger P, Serra A, Wagner C Renal expression of FGF23 and peripheral resistance to elevated FGF23 in rodent models of polycystic kidney disease. Kidney Int, 2014;85(6):1340-50. 2014 [PMID: 24402093] (Western Blot, Rat) Western Blot Rat
Show All 12 Publications.

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Product General Protocols

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FAQs for IgG Antibody (HAF005) (0)

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Other Available Formats

Biotin BAF005
HRP (Horseradish Peroxidase) HAF005
NL493 NL015
NL557 NL013
NL637 NL014

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CD16 - Find me on macrophages, neutrophils and NK cells
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Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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Gene Symbol IGHG1