IgG Antibody (97924) [Allophycocyanin]

Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP was stained with either (A) Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). Panel C shows HEK293 cell line transfected with human PD-1, untreated with B7-H1 Fc Chimera, and stained with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 97924
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

IgG Antibody (97924) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IgG₁ Fc region
• Specificity: Detects chimeric proteins containing a human IgG Fc region in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGHG1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IgG Antibody (97924) [Allophycocyanin]

• IgG
• Immunoglobulin G
• ImmunoglobulinG

Background

Human IgG Fc APC‑conjugated Antibody Monoclonal Mouse IgG1 Clone # 97924

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (FAB110A)(6)

Publications using FAB110A

• Hiroyasu Konno, Tracey Lin, Renyi Wu, Xinchuan Dai, Shou Li, Grace Wang et al. ZL-1211 Exhibits Robust Antitumor Activity by Enhancing ADCC and Activating NK Cell–mediated Inflammation in CLDN18.2-High and -Low Expressing Gastric Cancer Models Cancer Research Communications 2022-09-07 [PMID: 36922936] (Flow Cytometry, Human)
• M Hinterleit, C Hinterleit, E Malenke, B Federmann, U Holzer, M Müller, WA Bethge, S Wirths Early Reconstitution of Antibody Secreting Cells after Allogeneic Stem Cell Transplantation Journal of Clinical Medicine, 2022-01-05;11(1):. 2022-01-05 [PMID: 35012014] (Flow Cytometry, Human)
• GM Castiglion, L Zhou, Z Xu, Z Neiman, CF Hung, EJ Duh Evolutionary pathways to SARS-CoV-2 resistance are opened and closed by�epistasis acting on ACE2 PloS Biology, 2021-12-21;19(12):e3001510. 2021-12-21 [PMID: 34932561] (Flow Cytometry, Human)
• J Hunka, JT Riley, GF Debes Approaches to overcome flow cytometry limitations in the analysis of cells from veterinary relevant species BMC Vet. Res., 2020-03-06;16(1):83. 2020-03-06 [PMID: 32143631] (Flow Cytometry, Sheep)
• HG Wirsching, H Zhang, F Szulzewsky, S Arora, P Grandi, PJ Cimino, N Amankulor, JS Campbell, L McFerrin, SS Pattwell, C Ene, A Hicks, M Ball, J Yan, J Zhang, D Kumasaka, RH Pierce, M Weller, M Finer, C Quéva, JC Glorioso, AM Houghton, EC Holland Arming oHSV with ULBP3 drives abscopal immunity in lymphocyte-depleted glioblastoma JCI Insight, 2019-07-11;4(13):. 2019-07-11 [PMID: 31292299] (Flow Cytometry, Human, Mouse)
• T Yoshioka, T Tatsumi, T Miyagi, K Mukai, K Nishio, A Nishio, Y Yokoyama, T Suda, T Kegasawa, M Shigekawa, H Hikita, R Sakamori, T Takehara Frequency and role of NKp46 and NKG2A in hepatitis B virus infection PLoS ONE, 2017-03-22;12(3):e0174103. 2017-03-22 [PMID: 28328926] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IGHG1