Recombinant Human IGFBP-rp1/IGFBP-7 His Protein

SDS-Page: Recombinant Human IGFBP-rp1/IGFBP-7 Protein [NBP2-23142]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human IGFBP-rp1/IGFBP-7 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 27-282 of Human IGFBP-rp1/IGFBP-7
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSSSSDTCG PCEPASCPPL PPLGCLLGET RDACGCCPMC ARGEGEPCGG GGAGRGYCAP GMECVKSRKR RKGKAGAAAG GPGVSGVCVC KSRYPVCGSD GTTYPSGCQL RAASQRAESR GEKAITQVSK GTCEQGPSIV TPPKDIWNVT GAQVYLSCEV IGIPTPVLIW NKVKRGHYGV QRTELLPGDR DNLAIQTRGG PEKHEVTGWV LVSPLSKEDA GEYECHASNS QGQASASAKI TVVDALHEIP VKKGEGAEL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IGFBP7
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 28.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 50% glycerol, 2 mM DTT, 1 mM EDTA
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human IGFBP-rp1/IGFBP-7 His Protein

• angiomodulin
• FSTL2
• IBP-7
• IGFBP7
• IGFBP-7
• IGFBP-7PGI2-stimulating factor
• IGFBP-7v
• IGFBPrp1
• IGFBP-rp1
• insulin-like growth factor binding protein 7
• insulin-like growth factor-binding protein 7
• MAC25 protein
• Mac25
• MAC25IGF-binding protein 7
• Prostacyclin-stimulating factor
• PSF
• PSFAGM
• TAF
• Tumor-derived adhesion factor

Background

IGFBP7 is a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and one variant has been associated with retinal arterial macroaneurysm Recombinant human IGFBP7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Publications for IGFBP-rp1/IGFBP-7 Recombinant Protein (NBP2-23142)(1)

Publication using NBP2-23142

• Tirsa T. van Duijl, L. Renee Ruhaak, Nico P. M. Smit, Mervin M. Pieterse, Fred P. H. T. M. Romijn, Natasja Dolezal, Jan Wouter Drijfhout, Johan W. de Fijter, Christa M. Cobbaert Development and Provisional Validation of a Multiplex LC-MRM-MS Test for Timely Kidney Injury Detection in Urine Journal of Proteome Research 2021-11-04 [PMID: 34735145]

Bioinformatics

• Gene Symbol: IGFBP7