IGFBP-rp1/IGFBP-7 Antibody (192520) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 192520
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

IGFBP-rp1/IGFBP-7 Antibody (192520) [Alexa Fluor® 405] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IGFBP‑rp1/IGFBP-7
  Asp30-Arg277
  Accession # AAA16187
• Specificity: Detects human IGFBP‑rp1/IGFBP-7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human IGFBP-1, -2, -3, -4, -5, or -6 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
• Purity: Protein A or G purified

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGFBP-rp1/IGFBP-7 Antibody (192520) [Alexa Fluor® 405]

• angiomodulin
• FSTL2
• IBP-7
• IGFBP7
• IGFBP-7
• IGFBP-7PGI2-stimulating factor
• IGFBP-7v
• IGFBPrp1
• IGFBP-rp1
• insulin-like growth factor binding protein 7
• insulin-like growth factor-binding protein 7
• MAC25 protein
• Mac25
• MAC25IGF-binding protein 7
• Prostacyclin-stimulating factor
• PSF
• PSFAGM
• TAF
• Tumor-derived adhesion factor

Background

IGFBP-rp1, also known as Mac25/Angiomodulin (AGM), tumor-derived adhesion factor (TAF) and prostacyclin-stimulating factor (PSF), is a secreted protein that contains three protein domain modules. Human IGFBP-rp1 cDNA encodes 282 amino acid (aa) precursor protein with a putative 26 aa signal peptide. Mature
IGFBP-rp1 is a glycosylated protein with an N-terminal IGFBP domain, followed by a Kazal-type serine proteinase inhibitor domain and a C-terminal
immunoglobulin‑like C2-type domain. The similarity of IGFBP-rp1 with the IGFBPs is confined to the N-terminal IGFBP domain, which contains all 12 of the conserved cysteine residues found in IGFBP1 through 5. Human and mouse IGFBP-rP1 are highly homologous. Human and mouse IGFBP-rp1 share 94% aa sequence identity. IGFBP-rp1 is expressed in many normal tissues and in cancer cells. It is abundantly expressed in high endothelial venules (HEVs) of blood vessels in the secondary lymphoid tissues. The expression of IGFBP-rp1 is upregulated in senescing epithelial cells and by retinoic acid. IGFBP-rp1 binds IGF and insulin with very low affinity and has been shown to enhance the mitogenic actions of IGF and insulin. IGFBP-rp1 also has IGF/insulin-independent activities. It interacts with heparan sulfate proteoglycans, type IV collagen, and specific chemokines. IGFBP‑rp1 supports weak cell adhesion, promotes cell spreading on type IV collagen, and stimulates the production of the potent vasodilator PGI₂. It modulates tumor cell growth and has also been implicated in angiogenesis. IGFBP-rp1 is proteolytically cleaved between lysine 97 and alanine 98. Cleaved IGFBP-rp1 has enhanced cell attachment activity but can no longer bind IGF/insulin (1-3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.