IGFBP-5 Antibody (145619) [Unconjugated]

(A) Elisa determination of IGFBP-2 and -5 concentrations in conditioned medium (CM) from wt and TamR MCF-7 cell lines. Data represent mean ± SD (n = 3). Experiments were repeated on three separate occasions. *p < 0.001, **p < 0.0001 (Student's unpaired t-test). (B) Western blot (upper two panels) and Ligand blot (lower panel) identifies IGFBP-2 (upper arrow Ligand blot) and IGFBP-5 (lower panel Ligand blot) in CM from wt and TamR MCF-7 cells. (C) Densitometric analysis of Western blots for IGFBP-2 (upper graph) and IGFBP-5 (lower graph) band intensity. Triplicate lanes per blot were analysed for both wt and TamR conditioned media using Image Lab software. Data are presented as mean +/− SD (n = 3) of Arbitrary Unit (AU) intensity. This experiment was repeated 3 times with similar results in each instance. *p < 0.005 TamR v MCF-7 Students t-test. GraphPad Prism 5.0. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27050076), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, ELISA(Cap)
• Clone: 145619
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IGFBP-5 Antibody (145619) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IGFBP-5
  Glu28-Glu272
  Accession # P24593
• Specificity: Detects human IGFBP-5 in ELISAs and Western blots. In ELISAs, this antibody shows 5% cross-reactivity with recombinant mouse (rm) IGFBP‑5 and no cross‑reactivity with rhIGFBP-2, -3, -4, -6, rmIGFBP-1, -2, -3, -6, rhIGFBP-rp1, rmIGF-I, rmIGF-II, rhIGF-I, or rhIGF-II. In Western blots, this antibody does not cross-react with rhIGFBP-1, -2, -3, -4, -6, or -7.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGFBP5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human IGFBP-5 Biotinylated Antibody (Catalog number BAF875)
  Standard: Recombinant Human IGFBP-5 (Catalog number 875-B5)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGFBP-5 Antibody (145619) [Unconjugated]

• IBP5IBP-5
• IGF-binding protein 5
• IGFBP5
• IGFBP-5
• insulin-like growth factor binding protein 5
• insulin-like growth factor-binding protein 5

Background

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-transitional modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Human IGFBP-5 cDNA encodes a 272 amino acid (aa) residue precursor protein with a putative 20 aa residue signal peptide that is processed to generate the 252 aa residue mature protein that is post-translationally modified by O-glycosylations and serine phosphorylations. IGFBP-5 is expressed by fibroblasts, myoblasts and osteoblasts, making it the predominant IGFBP found in bone extracts. IGFBP-5 has a strong affinity for hydroxyapatite, allowing it to bind to bone cells. When bound to extracellular matrix, IGFBP-5 is protected from proteolysis and potentiates IGF activity, but when it is soluble, IGFBP-5 is cleaved to a biologically inactive 21 kDa fragment.

1. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
2. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGFBP-5 Antibody (MAB8751)(2)

Publications using MAB8751

• Deregulation of IGF-binding proteins -2 and -5 contributes to the development of endocrine resistant breast cancer in vitro Oncotarget, 2016-05-31;7(22):32129-43. 2016-05-31 [PMID: 27050076] (Western Blot, Human)
• Hung PS, Kao SY, Shih YH, Chiou SH, Liu CJ, Chang KW, Lin SC Insulin-like growth factor binding protein-5 (IGFBP-5) suppresses the tumourigenesis of head and neck squamous cell carcinoma. J. Pathol., 2008-02-01;214(3):368-76. 2008-02-01 [PMID: 18085517] (Western Blot, Human)

Bioinformatics

• Gene Symbol: IGFBP5
• Uniprot:
  • P24593()