IGFBP-4 Overexpression Lysate


Western Blot: IGFBP4 Overexpression Lysate (Adult Normal) [NBL1-11874] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IGFBP4.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

IGFBP-4 Overexpression Lysate Summary

Transient overexpression lysate of insulin-like growth factor binding protein 4 (IGFBP4) The lysate was created in HEK293T cells, using Plasmid ID RC204188 and based on accession number NM_001552. The protein contains a C-MYC/DDK Tag.


Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
25.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array



HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for IGFBP-4 Overexpression Lysate

  • BP-4
  • HT29-IGFBP
  • IBP4insulin-like growth factor-binding protein 4
  • IGF-binding protein 4
  • IGFBP4
  • IGFBP-4
  • IGFBP-4IBP-4
  • insulin-like growth factor binding protein 4


This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ELISA(Cap), ELISA(Det), ELISA(Sta)
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu
Applications: WB, Flow, IHC, ELISA(Cap), ELISA(Det), ICC, Neut, ELISA(Sta)
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, Neut
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, Neut
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P

Publications for IGFBP-4 Lysate (NBL1-11874) (0)

There are no publications for IGFBP-4 Lysate (NBL1-11874).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IGFBP-4 Lysate (NBL1-11874) (0)

There are no reviews for IGFBP-4 Lysate (NBL1-11874). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IGFBP-4 Lysate (NBL1-11874) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats


Additional IGFBP-4 Products

Bioinformatics Tool for IGFBP-4 Lysate (NBL1-11874)

Discover related pathways, diseases and genes to IGFBP-4 Lysate (NBL1-11874). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IGFBP-4 Lysate (NBL1-11874)

Discover more about diseases related to IGFBP-4 Lysate (NBL1-11874).

Pathways for IGFBP-4 Lysate (NBL1-11874)

View related products by pathway.

PTMs for IGFBP-4 Lysate (NBL1-11874)

Learn more about PTMs related to IGFBP-4 Lysate (NBL1-11874).

Blogs on IGFBP-4

There are no specific blogs for IGFBP-4, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IGFBP-4 Antibody

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our IGFBP-4 Overexpression Lysate and receive a gift card or discount.


Gene Symbol IGFBP4