Recombinant Human IGFBP-4 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Concentration
LYOPH

Order Details

Recombinant Human IGFBP-4 Protein Summary

Description
A biologically active protein to IGFBP-4 that contains 237 amino acids.

Source: Insect Cell

Amino Acid Sequence:
DEAIHCPPCS EEKLARCRPP VGCEELVREP GCGCCATCAL GLGMPCGVYT PRCGSGLRCY PPRGVEKPLH TLMHGQGVCM ELAEIEAIQE SLQPSDKDEG DHPNNSFSPC SAHDRRCLQK HFAKIRDRST SGGKMKVNGA PREDARPVPQ GSCQSELHRA LERLAASQSR THEDLYIIPI PNCDRNGNFH PKQCHPALDG QRGKCWCVDR KTGVKLPGGL EPKGELDCHQ LADSFRE

Details of Functionality
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells is less than 0.1 ug/ml, corresponding to a specific activity of > 1.0 x 104 IU/mg in the presence of 14 ng/ml of rHuIGF-II.
Source
Baculovirus
Protein/Peptide Type
Recombinant Protein
Gene
IGFBP4
Purity
>97%, by SDS-PAGE with silver staining
Endotoxin Note
Less than 0.1 EU/ug of rHuIGF-BP4, Insect Cell as determined by LAL method.

Applications/Dilutions

Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 20mM Tris-HCl, pH 8.0, 150mM NaCl.
Preservative
No Preservative
Concentration
LYOPH
Purity
>97%, by SDS-PAGE with silver staining
Reconstitution Instructions
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Alternate Names for Recombinant Human IGFBP-4 Protein

  • BP-4
  • HT29-IGFBP
  • IBP4insulin-like growth factor-binding protein 4
  • IGF-binding protein 4
  • IGFBP4
  • IGFBP-4
  • IGFBP-4IBP-4
  • insulin-like growth factor binding protein 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol IGFBP4
Entrez