IGFBP-4 Antibody (82314) [Unconjugated]

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Recombinant Human IGFBP‑4 (Catalog # 804-GB) inhibits Recombinant Human IGF‑II (Catalog # 292-G2) induced proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clone
82314
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

IGFBP-4 Antibody (82314) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGFBP‑4
Asp22-Glu258
Accession # AAA62670
Specificity
Detects human IGFBP-4 in ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IGFBP4
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 20-80 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human IGFBP-4 Biotinylated Antibody (Catalog number BAF804)
Standard: Recombinant Human IGFBP-4 (Catalog number 804-GB)
Publications
Read Publication using
MAB8041 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGFBP-4 Antibody (82314) [Unconjugated]

  • BP-4
  • HT29-IGFBP
  • IBP4insulin-like growth factor-binding protein 4
  • IGF-binding protein 4
  • IGFBP4
  • IGFBP-4
  • IGFBP-4IBP-4
  • insulin-like growth factor binding protein 4

Background

Human IGF binding protein 4 (IGFBP-4) was isolated from human plasma based on its ability to bind immobilized IGF-I. Human IGFBP-4 cDNA encodes a 258 amino acid (aa) residue precursor protein with a predicted 21 aa residue signal peptide that is processed to generate the 237 aa residue mature human IGFBP-4. The human IGFBP-4 contains a potential N-linked glycosylation site and shares approximately 90% amino acid sequence identity with both the mouse and rat IGFBP-4. According to the nomenclature of IGFBPs defined at the 4th International Symposium of IGFs (1997, Tokyo), six high-affinity IGF binding proteins (IGFBP-1, -2, -3, 4, -5, -6), and four IGFBP-related proteins (IGFBPr-1, - 2, -3, -4) have been identified. All IGFBPs have a high cysteine content and share conserved cysteine residues that are clustered in the amino- and carboxy-terminal third of the molecule. IGFBPs have been shown to either inhibit or enhance the biological activities of IGF, or act in an IGF-independent manner. Post-translational modification of IGFBPs, including phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins for IGF and may indirectly regulate IGF actions.

  1. Jones, J.I. and D.R. Clemons (1995) Endocrine Rev. 16:3.
  2. Kelly, K.M. et al. (1996) Intl. J. Biochem. Cell Biol. 28:619.
  3. Kim, H-S. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12981.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IGFBP-4 Antibody (MAB8041)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA Development.


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Bioinformatics

Gene Symbol IGFBP4
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