IGFBP-3 Antibody (MM0342-6U23) - Azide and BSA Free

• Reactivity: Hu
• Applications: WB, IHC
• Clone: MM0342-6U23
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

IGFBP-3 Antibody (MM0342-6U23) - Azide and BSA Free Summary

• Immunogen: Human recombinant IGFBP-3
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGFBP3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20-200
  • Immunohistochemistry-Paraffin 1:20-1:200
  • Western Blot 1:500-1:2000
• Application Notes: This IGFBP3 antibody (clone MM0342-6U23) is useful for Western Blot and Immunohistochemistry-Paraffin applications. In its IHC-P validation, the antigen retrieval was performed by incubation of sections with Proteinase K (20ug/ml in PBS) for 15min at room temperature (Note: the length of PK exposure should be optimized by the user as it may depend upon the variation of fixatives and length of fixation applied to a particular specimen).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for IGFBP-3 Antibody (MM0342-6U23) - Azide and BSA Free

• acid stable subunit of the 140 K IGF complex
• binding protein 29
• binding protein 53
• growth hormone-dependent binding protein
• IBP-3
• IBP3BP-53
• IGF-binding protein 3
• IGFBP3
• IGFBP-3
• insulin-like growth factor binding protein 3
• insulin-like growth factor-binding protein 3

Background

IGFBP3 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGFBP-3 Antibody (NBP2-12364)(2)

Publications using NBP2-12364

• Lee D, Ahn J, Koo HS, Kang YJ. Intrauterine botulinum toxin A administration promotes endometrial regeneration mediated by IGFBP3-dependent OPN proteolytic cleavage in thin endometrium Cellular and Molecular Life Sciences 2023-01-05 [PMID: 36602651] (Immunohistochemistry-Paraffin, Human)
• Kim N, Kim HK, Lee K et al. Single-cell RNA sequencing demonstrates the molecular and cellular reprogramming of metastatic lung adenocarcinoma Nat Commun 2020-05-08 [PMID: 32385277] (IHC-P, Human)

Bioinformatics

• Gene Symbol: IGFBP3
• Uniprot:
  • CAA46087()