IGFBP-1 Antibody (33627.11)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ELISA
Clone
33627.11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

IGFBP-1 Antibody (33627.11) Summary

Immunogen
Recombinant full length human insulin-like growth factor binding protein 1, expressed in Escherichia coli.
Localization
Secreted
Specificity
No cross-reactivity is seen with recombinant human IGFBP2, IGFBP3 and IGFBP4.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IGFBP1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Blocking/Neutralizing 50-200 molar excess
  • ELISA 2 ug/ml
Application Notes
ELISA: Use at a concentration of 2 ug/ml. In the ELISA capture assay, plates are coated with 100 ul/well of the capture antibody (2 ug/ml) in combination with 100 ul/well of a detection antibody (affinity-purified biotinylated polyclonal anti-human IGFBP1 antibody at 100 ng/ml). An ELISA range of 62.4 to 4000 pg/ml may be obtained. Neut: Use at a concentration of 10 - 40 ug/ml. The Neutralization Dose50 (ND50) for this antibody is 10-40 ug/ml in the presence of approximately 5 ug/ml of recombinant human IGFBP1 and approximately 6 ng/mL of recombinant human IGF1, using human MCF7 cells. WB: Use at a concentration of 1-2 ug/ml. The detection limit for recombinant human IGFBP1 is approximately 0.5 ng/lane under non-reducing conditions. ot tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Positive Control
IGFBP-1 Lysate (NBL1-11873)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for IGFBP-1 Antibody (33627.11)

  • alpha-pregnancy-associated endometrial globulin
  • amniotic fluid binding protein
  • binding protein-25
  • binding protein-26
  • binding protein-28
  • growth hormone independent-binding protein
  • hIGFBP-1
  • IBP1
  • IBP-1
  • IGF-binding protein 1
  • IGFBP1
  • IGFBP-1
  • IGF-BP25
  • insulin-like growth factor binding protein 1
  • insulin-like growth factor-binding protein 1
  • Placental protein 12
  • PP12AFBP

Background

Insulin-like growth factor binding protein-1 (IGFBP-1) is a member of the superfamily of insulin-like growth factor (IGF) binding proteins which include six high-affinity IGF binding proteins (IGFBP) and at least four low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). The IGFBP members are cysteine-rich proteins with conserved cysteine residues, clustered in the amino-terminal and the carboxyterminal regions of the molecule. Contained within IGFBP-1 and

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IGFBP1
Entrez