IGFBP-1 Antibody (33627.11) Summary
Recombinant full length human insulin-like growth factor binding protein 1, expressed in Escherichia coli.
No cross-reactivity is seen with recombinant human IGFBP2, IGFBP3 and IGFBP4.
Protein G purified
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Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Protein G purified
ELISA: Use at a concentration of 2 ug/ml. In the ELISA capture assay, plates are coated with 100 ul/well of the capture antibody (2 ug/ml) in combination with 100 ul/well of a detection antibody (affinity-purified biotinylated polyclonal anti-human IGFBP1 antibody at 100 ng/ml). An ELISA range of 62.4 to 4000 pg/ml may be obtained. Neut: Use at a concentration of 10 - 40 ug/ml. The Neutralization Dose50 (ND50) for this antibody is 10-40 ug/ml in the presence of approximately 5 ug/ml of recombinant human IGFBP1 and approximately 6 ng/mL of recombinant human IGF1, using human MCF7 cells. WB: Use at a concentration of 1-2 ug/ml. The detection limit for recombinant human IGFBP1 is approximately 0.5 ng/lane under non-reducing conditions. ot tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Alternate Names for IGFBP-1 Antibody (33627.11)
- alpha-pregnancy-associated endometrial globulin
- amniotic fluid binding protein
- binding protein-25
- binding protein-26
- binding protein-28
- growth hormone independent-binding protein
- IGF-binding protein 1
- insulin-like growth factor binding protein 1
- insulin-like growth factor-binding protein 1
- Placental protein 12
Insulin-like growth factor binding protein-1 (IGFBP-1) is a member of the superfamily of insulin-like growth factor (IGF) binding proteins which include six high-affinity IGF binding proteins (IGFBP) and at least four low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). The IGFBP members are cysteine-rich proteins with conserved cysteine residues, clustered in the amino-terminal and the carboxyterminal regions of the molecule. Contained within IGFBP-1 and
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
Applications: WB, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
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Positive Control Lysate(s)
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