IGFBP-1 Antibody (33627.11)

Product Discontinued

IGFBP-1 Antibody (33627.11) Summary

• Immunogen: Recombinant full length human insulin-like growth factor binding protein 1, expressed in Escherichia coli.
• Localization: Secreted
• Specificity: No cross-reactivity is seen with recombinant human IGFBP2, IGFBP3 and IGFBP4.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGFBP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA 2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA: Use at a concentration of 2 ug/ml. In the ELISA capture assay, plates are coated with 100 ul/well of the capture antibody (2 ug/ml) in combination with 100 ul/well of a detection antibody (affinity-purified biotinylated polyclonal anti-human IGFBP1 antibody at 100 ng/ml). An ELISA range of 62.4 to 4000 pg/ml may be obtained. Neut: Use at a concentration of 10 - 40 ug/ml. The Neutralization Dose50 (ND50) for this antibody is 10-40 ug/ml in the presence of approximately 5 ug/ml of recombinant human IGFBP1 and approximately 6 ng/mL of recombinant human IGF1, using human MCF7 cells. WB: Use at a concentration of 1-2 ug/ml. The detection limit for recombinant human IGFBP1 is approximately 0.5 ng/lane under non-reducing conditions. ot tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
• Control:

  IGFBP-1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for IGFBP-1 Antibody (33627.11)

• alpha-pregnancy-associated endometrial globulin
• amniotic fluid binding protein
• binding protein-25
• binding protein-26
• binding protein-28
• growth hormone independent-binding protein
• hIGFBP-1
• IBP1
• IBP-1
• IGF-binding protein 1
• IGFBP1
• IGFBP-1
• IGF-BP25
• insulin-like growth factor binding protein 1
• insulin-like growth factor-binding protein 1
• Placental protein 12
• PP12AFBP

Background

Insulin-like growth factor binding protein-1 (IGFBP-1) is a member of the superfamily of insulin-like growth factor (IGF) binding proteins which include six high-affinity IGF binding proteins (IGFBP) and at least four low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). The IGFBP members are cysteine-rich proteins with conserved cysteine residues, clustered in the amino-terminal and the carboxyterminal regions of the molecule. Contained within IGFBP-1 and

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGFBP1
• Entrez:
  • 3484(Human)