IGF-I R/IGF1R Antibody (3G5C1)

Western Blot: IGF-I R Antibody (3G5C1) [NB110-87052] - Analysis using IGF1R-Beta mouse mAb against truncated IGF1R recombinant protein.

Immunohistochemistry: IGF-I R/IGF1R Antibody (3G5C1) [NB110-87052] - Expression of growth factor receptors in male breast cancer. Expression of HER2, FGFR2, EGFR, IGF-I R/IGF1R, and MET in representative cases of male breast cancer. Size bars equal 20 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0053353) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: IGF-I R Antibody (3G5C1) [NB110-87052] - Analysis of ovarian cancer tissues using IGF1R-Beta mouse mAb with DAB staining.

Immunohistochemistry-Paraffin: IGF-I R Antibody (3G5C1) [NB110-87052] - Analysis of rectum cancer tissues using IGF1R-Beta mouse mAb with DAB staining.

Expression of growth factor receptors in male breast cancer.Expression of HER2, FGFR2, EGFR, IGF1-R, and MET in representative cases of male breast cancer. Size bars equal 20 µm.

Representative IHC staining in primary breast tumors (left; no membrane staining for Claudin-4, CAIX and GLUT-1 (significant negative to positive conversion), positive membrane staining for EGFR and IGF1R (negative to positive conversion n.s.) and metastases (right; positive membrane staining). Magnification: 20× (inlet 40×).

Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (3G5C1) - BSA Free [NB110-87052] - Representative IHC staining in primary breast tumors (left; no membrane staining for Claudin-4, CAIX & GLUT-1 (significant negative to positive conversion), positive membrane staining for EGFR & IGF1R (negative to positive conversion n.s.) & metastases (right; positive membrane staining). Magnification: 20× (inlet 40×). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25417118), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 3G5C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IGF-I R/IGF1R Antibody (3G5C1) Summary

• Description: Novus Biologicals Mouse IGF-I R/IGF1R Antibody (3G5C1) (NB110-87052) is a monoclonal antibody validated for use in IHC, WB and ELISA. Anti-IGF-I R/IGF1R Antibody: Cited in 10 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of IGF-I R/IGF1R (AA: 1101-1367) expressed in E. Coli.
• Localization: Membrane; Single-pass type I membrane protein.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGF1R
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 96 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for IGF-I R/IGF1R Antibody (3G5C1)

• CD221 antigen
• CD221
• EC 2.7.10
• EC 2.7.10.1
• IGF1R
• IGF-1R
• IGF-I R
• IGF-I receptor
• IGFIR
• IGF-IR
• IGFR
• insulin-like growth factor 1 receptor
• Insulin-like growth factor I receptor
• JTK13
• MGC142170
• MGC142172
• MGC18216
• soluble IGF1R variant 1
• soluble IGF1R variant 2

Background

IGF1 Receptor (insulin-like growth factor 1 receptor, IGF1R), a transmembrane receptor tyrosine kinase, is widely expressed in many cell types within fetal and postnatal tissues, and in many cell lines. Upon binding to its ligands, IGF1 and IGF2, receptor autophosphorylation occurs. The triple tyrosine cluster within the kinase domain (Tyr1131, Tyr1135 and Tyr1136) is the earliest major site of autophosphorylation. Phosphorylation of these three tyrosine residues is necessary for kinase activation. Insulin receptors (IRs) share significant similarity with IGF1 receptors in both structure and function,including an equivalent triple tyrosine cluster within the activation loop of the kinase domain (Tyr1146, Tyr1150 and Tyr1151).Tyrosine autophosphorylation of insulin receptor is one of the earliest cellular responses to insulin stimulation. Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151. Full kinase activation requires the triple tyrosine phosphorylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGF-I R/IGF1R Antibody (NB110-87052)(10)

Publications using NB110-87052

• De Martino MC, van Koetsveld PM, Feelders RA et al. IGF and mTOR pathway expression and in vitro effects of linsitinib and mTOR inhibitors in adrenocortical cancer Endocrine 2019-03-05 [PMID: 30838516] (IHC-P, Human)
• Wang H, Huang W, Yu X et al. Two prostate cancer‑associated polymorphisms in the 3'UTR of IGF1R influences prostate cancer susceptibility by affecting miRNA binding. Oncol. Rep. 2018-10-22 [PMID: 30365147] (WB)
• Jori B, Kamps R, Xanthoulea S et al. Germ-line variants identified by next generation sequencing in a panel of estrogen and cancer associated genes correlate with poor clinical outcome in Lynch syndrome patients. Oncotarget. 2015-10-22 [PMID: 26517685] (IHC-P, Human)
• Koo DH, Lee HJ, Ahn JH et al. Tau and PTEN status as predictive markers for response to trastuzumab and paclitaxel in patients with HER2-positive breast cancer Tumour Biol. 2015-03-01 [PMID: 25725586] (IHC-P, Human)
• Jiwa Ls, Van Diest Pj, Hoefnagel Ld et al. Upregulation of Claudin-4, CAIX and GLUT-1 in distant breast cancer metastases. BMC Cancer. 2014-11-27 [PMID: 25417118] (IHC-P, Human)
• Vermeulen JF, van der Wall E, Witkamp AJ, van Diest PJ. Analysis of expression of membrane-bound tumor markers in ductal carcinoma in situ of the breast: paving the way for molecular imaging. Cell Oncol (Dordr) 2013-06-07 [PMID: 23744486]
• Vermeulen JF, Kornegoor R, van der Wall E et al. Differential expression of growth factor receptors and membrane-bound tumor markers for imaging in male and female breast cancer. PLoS One. 2013-01-04 [PMID: 23308200] (IF/IHC, Human)
• Varewijck AJ, Brugts MP, Frystyk J et al. Circulating insulin-like growth factors may contribute substantially to insulin receptor isoform A and insulin receptor isoform B signalling Mol Cell Endocrinol 2012-09-07 [PMID: 22982059] (IF/IHC, Human)
• Vermeulen JF, van Brussel AS, van der Groep P et al. Immunophenotyping invasive breast cancer: paving the road for molecular imaging. BMC Cancer 2012-06-01 [PMID: 22695343] (IF/IHC, Human)
• Kong KL, Kwong DL, Chan TH, Law SY, Chen L, Li Y, Qin YR, Guan XY. MicroRNA-375 inhibits tumour growth and metastasis in oesophageal squamous cell carcinoma through repressing insulin-like growth factor 1 receptor. Gut;61(1):33-42. 2012-01-01 [PMID: 21813472] (WB, Human)

Reviews for IGF-I R/IGF1R Antibody (NB110-87052) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Rhesus Macaque.

reviewed by: Paula Aliberti IHC-P Rhesus Macaque 11/24/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Infant testis
• Species: Rhesus Macaque
• Lot: 130312

reviewed by: Paula Aliberti IHC-P Human 10/09/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human testis
• Species: Human
• Lot: 130312

Comments

• Comments: antibody dilution 1/50

FAQs for IGF-I R/IGF1R Antibody (NB110-87052). (Showing 1 - 1 of 1 FAQs).

1. I ordered NB110-87052 and the website said that the concentration is mentioned on the vial, but it is not. Can you tell me what's the (antibody) concentration in Lot 81210 and 3G5C1?
   • The formulation for the product is ascites fluid therefore the concentration is unknown because we cannot typically measure an accurate concentration with ascites that hasn't been purified.

Bioinformatics

• Gene Symbol: IGF1R
• Entrez:
  • 3480(Human)
• Uniprot:
  • P08069()