Recombinant Human IGF-I/IGF-1 Protein Summary
| Description |
Recombinant bioactive protein containing 70 amino acids for Human IGF-I Source:E. coli Amino Acid Sequence:(P01343) |
Preparation Method |
Determined to be >98% pure by SDS-PAGE |
| Details of Functionality |
EC50 is typically 21.6-54.7 ng/ml. The activity was determined using dose dependent stimulation of Balbc 3T3 cell line. |
| Source |
E. coli |
| Protein/Peptide Type |
Recombinant Protein |
| Gene |
IGF1 |
| Purity |
>98%, by SDS-PAGE |
| Endotoxin Note |
<0.1 ng/ug |
Applications/Dilutions
| Dilutions |
|
| Theoretical MW |
7.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer |
Lyophilized with no additives. |
| Concentration |
Lyoph |
| Purity |
>98%, by SDS-PAGE |
| Reconstitution Instructions |
Reconstitute in H2O to a concentration of 0.1 - 1.0 mg/ml. This solution can be diluted into other buffered solutions or stored at 4C for 1 week or -20C for long-term storage. |
Alternate Names for Recombinant Human IGF-I/IGF-1 Protein
Background
IGF-I (insulin-like Growth Factor-I) is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. Human IGF-I is a 7.6 kDa protein containing 70 amino acid residues. The recombinant IGF-1 was produced using animal origin free technology.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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