Rat IGF-I/IGF-1 ELISA Kit (Colorimetric)

ELISA: Rat IGF-I ELISA Kit (Colorimetric) [NBP2-60060] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Rat IGF-I/IGF-1 ELISA Kit (Colorimetric) [NBP2-60060] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell culture supernates and tissue homogenates
• Standard Curve Range: 0.156 ng/mL - 10 ng/mL
• Sensitivity: 0.151 ng/mL

Rat IGF-I/IGF-1 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat IGF-1. No significant cross-reactivity or interference between rat IGF-1 and analogues was observed.
• Standard Curve Range: 0.156 ng/mL - 10 ng/mL
• Sensitivity: 0.151 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 8
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IGF1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat IGF-I/IGF-1 ELISA Kit (Colorimetric)

• IBP1
• IGF1
• IGF-1
• IGF1A
• IGFI
• IGF-I
• IGF-IA
• IGF-IB
• insulin-like growth factor 1 (somatomedin C)
• insulin-like growth factor 1
• insulin-like growth factor I
• insulin-like growth factor IA
• insulin-like growth factor IB
• Mechano growth factor
• MGF
• Somatomedin A
• Somatomedin C
• somatomedin-C

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGF-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGF-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGF-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGF-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IGF1