Monkey IgE ELISA Kit (Colorimetric)

ELISA: Monkey IgE ELISA Kit (Colorimetric) [NBP3-23448] - Samples were spiked with high concentrations of Monkey IgE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Monkey IgE ELISA Kit (Colorimetric) [NBP3-23448] - Standard Curve Reference

• Reactivity: Pm
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 62.50 - 4000 pg/mL

Monkey IgE ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Monkey IgE in samples. No significant cross-reactivity or interference between Monkey IgE and analogues was observed.
• Standard Curve Range: 62.50 - 4000 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 6.49
• Intra-Assay: %CV < 5.66
• Spike Recovery: 84 - 109%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IGHE

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Monkey IgE ELISA Kit (Colorimetric)

• IgE
• immunoglobulin heavy constant epsilon

Background

IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are ~150 ng/ml, compared to 10 mg/ml for the IgGs - it is capable of triggering the most powerful immune reactions. Most of our knowledge of IgE has come from research into the mechanism of a form of allergy known as type 1 hypersensitivity. There is much speculation into what physiological benefits IgE contributes, and so far, circumstantial evidence in animal models and statistical population trends have hinted that IgE may be beneficial in fighting gut parasites such as Schistosoma mansoni, but this has not been conclusively proven in humans. IgE may play an important role in the immune system's recognition of cancer, in which the stimulation of a strong cytotoxic response against cells displaying only small amounts of early cancer markers would be beneficial. IgE may be an important target in treatments for allergy and asthma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IgE ELISA Kit (NBP3-23448). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IGHE