Human IgE ELISA Kit (Colorimetric)

ELISA: Human IgE ELISA Kit (Colorimetric) [NBP2-60059]

Human IgE ELISA Kit (Colorimetric) [NBP2-60059] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum and plasma
• Standard Curve Range: 62.5 ng/mL - 4000 ng/mL
• Sensitivity: 3.640 ng/mL

Human IgE ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of human IgE. No significant cross-reactivity or interference between human IgE and analogues was observed.
• Standard Curve Range: 62.5 ng/mL - 4000 ng/mL
• Sensitivity: 3.640 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 8
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IGHE

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human IgE ELISA Kit (Colorimetric)

• IgE
• immunoglobulin heavy constant epsilon

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IgE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IgE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IgE bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IGHE