IGBP1 Antibody - BSA Free

Immunoprecipitation: IGBP1 Antibody [NB100-487] - Detection of Alpha-4 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate (50 mcg) from Ramos (R), K562 (K) and mouse NIH3T3 (M) cells. B. Whole cell lysate (1 mg/IP; 1/4 of IP reaction loaded/lane) from Ramos cells. Antibodies: NB 100-487 used at 0.4 mcg/ml (A) and 1 mcg/ml (B) for WB at 3 mcg/mg lysate for IP. Alpha-4 was also immunoprecipitated by NB 100-486, but not by another Alpha-4 antibody, which are both affinity purified antibodies to upstream epitopes on Alpha-4. Detection: Chemiluminescence with an exposure time of 30 seconds (A and B).

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

IGBP1 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 300 and the C-terminus (residue 339) of human Protein Phosphatase 2A, regulatory subunit alpha-4 (Immunoglobulin binding protein 1) using the numbering given in SwissProt entry P78318 (GeneID 3476).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGBP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:1000-1:10000
• Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:1,000 to 1:10,000. Immunoprecipitation: 2 to 5 ug/mg lysate. Histo/Cytochemistry: not tested. In some cases, the antibody may be diluted further than indicated.
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate
  IGBP1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IGBP1 Antibody - BSA Free

• ALPHA-4
• B cell signal transduction molecule alpha 4
• bA351K23.1 (immunoglobulin binding protein 1 (CD79A) )
• B-cell signal transduction molecule alpha 4
• CD79a-binding protein 1
• IBP1
• immunoglobulin (CD79A) binding protein 1
• immunoglobulin-binding protein 1
• Protein alpha-4
• protein phosphatase 2A, regulatory subunit alpha-4
• Renal carcinoma antigen NY-REN-16

Background

The proliferation and differentiation of B cells is dependent upon a B-cell antigen receptor (BCR) complex. Binding of antigens to specific B-cell receptors results in a tyrosine phosphorylation reaction through the BCR complex and leads to multiple signal transduction pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGBP1 Antibody (NB100-487)(3)

Publications using NB100-487

• Chen L, Lai Y, Zhu X et al. The role of specific PP2A complexes in the dephosphorylation of gamma-H2AX. J Cell. Sci. 2014-09-18 [PMID: 25236601]
• Collison A, Hatchwell L, Verrills N et al. The E3 ubiquitin ligase midline 1 promotes allergen and rhinovirus-induced asthma by inhibiting protein phosphatase 2A activity. Nat Med 2013-02-01 [PMID: 23334847] (IP, Human)
• LeNoue-Newton M, Watkins GR, Zou P et al. The E3 ubiquitin ligase- and protein phosphatase 2A (PP2A)-binding domains of the Alpha4 protein are both required for Alpha4 to inhibit PP2A degradation. J Biol Chem 2011-05-01 [PMID: 21454489]

Bioinformatics

• Gene Symbol: IGBP1
• Entrez:
  • 3476(Human)
  • 18518(Mouse)
• Uniprot:
  • P78318(Human)