Goat anti-Human Kappa Light Chain Secondary Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463] - JJN3 and 8226 Human myeloma cells stained with NB7463. Cells fixed in 4%PFA 10min, wash PBS X 3. Permeabilized ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Catalog# & Conjugate Size Price

Goat anti-Human Kappa Light Chain Secondary Antibody Summary

Immunogen
Human Kappa Light Chain
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with kappa light chains common to all human immunoglobulins. No was detected against lambda light chain or non-immunoglobulin serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGKC
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:30000
  • ELISA 1:1000 - 1:30000
  • Immunocytochemistry/Immunofluorescence 1:200- 1:2000
  • Immunohistochemistry 1:200- 1:2000
  • Immunohistochemistry-Paraffin 1:200- 1:2000
Application Notes
ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500
Reviewed Applications
Read 1 Review rated 5
using
NB7463 in the following applications:

Publications
Read Publications using NB7463.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Goat anti-Human Kappa Light Chain Secondary Antibody

  • HCAK1
  • IGKCD
  • immunoglobulin kappa constant
  • Km
  • MGC111575
  • MGC62011
  • MGC72072
  • MGC88770
  • MGC88771
  • MGC88809

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Kappa Light Chain Antibody (NB7463)(8)

We have publications tested in 1 confirmed species: Human.


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Human
(2)
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Showing Publications 1 - 8 of 8.
Publications using NB7463 Applications Species
Smith B, Kiessling A, Lledo-Garcia R et al. Generation and characterization of a high affinity anti-human FcRn antibody, rozanolixizumab, and the effects of different molecular formats on the reduction of plasma IgG concentration MAbs Aug 21 2018 [PMID: 30130439] (Human) Human
Kiessling P, Lledo-Garcia R, Watanabe S et al. The FcRn inhibitor rozanolixizumab reduces human serum IgG concentration: A randomized phase 1 study Sci Transl Med. 2017 Nov 01 [PMID: 29093180] (Human) Human
Richards FM, Tape CJ, Jodrell DI et al. Anti-tumour effects of a specific anti-ADAM17 antibody in an ovarian cancer model in vivo. PLoS One. 2012 [PMID: 22792380]

Details:
Citation using the HRP form of this antibody.
Wang G, Ujiie H, Shibaki A et al. Blockade of autoantibody-initiated tissue damage by using recombinant fab antibody fragments against pathogenic autoantigen. Am J Pathol. [PMID: 20042683]

Details:
Citation using the HRP form of this antibody.
Suzuki N, Aoki D, Tamada Y et al. HMOCC-1, a human monoclonal antibody that inhibits adhesion of ovarian cancer cells to human mesothelial cells. Gynecol Oncol. [PMID: 15491748]

Details:
Citation using the HRP form of this antibody.
Mukherjee J, Chios K, Fishwild D et al. Production and characterization of protective human antibodies against Shiga toxin 1. Infect Immun. [PMID: 12228326]

Details:
Citation using the HRP form of this antibody.
Mukherjee J, Chios K, Fishwild D et al. Human Stx2-specific monoclonal antibodies prevent systemic complications of Escherichia coli O157:H7 infection. Infect Immun. [PMID: 11796590]

Details:
Citation using the HRP form of this antibody.
Tomokiyo K, Teshima K, Nakatomi Y et al. Induction of acquired factor IX inhibitors in cynomolgus monkey (Macaca fascicularis): a new primate model of hemophilia B. Thromb Res. [PMID: 11369429]

Details:
Citation using the HRP form of this antibody.

Review for Kappa Light Chain Antibody (NB7463) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB7463:
Filter by Applications
ICC
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Kappa Light Chain NB7463
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reviewed by:
Natalie Erdmann
ICC Human 04/08/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedJJN3
SpeciesHuman
LotA6

Comments

Comments1. Cells fixed in 4%PFA 10 minutes, wash PBS X 3
2. Permeabilized 0.5% Triton X-100 5 minutes; wash PBS X 3
3. Block 10% donkey serum 1 hour
4. 1/10,000 NB7463 O/N @4oC; wash PBS X 3
5. 1/2000 donkey F(ab’)2 Alexa Fluor Cy3 anti-goat IgG (H+L) 1 hour @room temp (Jackson # 705-166-147); wash PBS X 3
6. Olympus/BIOVIEW Cy3 filter 300ms exposure

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Kappa Light Chain Antibody (NB7463) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Alkaline Phosphatase NB7464
FITC NB7465
HRP NB7466

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Recent Reviews

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5
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1
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Natalie Erdmann
04/08/2019
Application: ICC
Species: Human

Bioinformatics

Gene Symbol IGKC