IFN-kappa Antibody (1009725) [Unconjugated]

IFN‑ kappa was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human IFN‑ kappa Monoclonal Antibody (Catalog # MAB102471) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in keratinocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 1009725
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IFN-kappa Antibody (1009725) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IFN‑ kappa
  Leu28-Lys207
  Accession # Q90W0
• Specificity: Detects human IFN‑ kappa in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-kappa Antibody (1009725) [Unconjugated]

• IFNK
• IFNkappa
• IFN-kappa

Background

Interferon kappa, also known as IFN-kappa, is a 207 aminoacids protein that in humans is encoded by the IFNK gene. IFN-kappa is a member of the type I interferon family which also includes IFN-alpha, -beta, -epsilon, and -omega. Type I interferons are a group of related glycoproteins that play an important role in host defenses against viral infections. Human IFN-kappa has been detected in keratinocytes, monocytes, and monocyte-derived dendritic cells and is reported to have contact-dependent antiviral activity. Human papillomavirus (HPV) 16 oncogene expression, which is necessary for the development of cervical cancer, has been shown to down-regulate human IFN-kappa expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q90W0()