Recombinant Human IFN-gamma R2 Protein

Product Discontinued

Recombinant Human IFN-gamma R2 Protein Summary

• Description: A biologically active protein to IFNGR2.
• Specificity: IFN-gamma R2 - Fc HEX Chimera has N- and possibly O-linked oligosaccharides. IFN-gamma R2 - Fc HEX Chimera migrates as a broad band between 65 and 75 kDa on SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified IFN-gamma R2 - Fc Chimera that has a predicted molecular mass of 52 kDa.
• Preparation Method: DNA encoding the signal peptide and extracellular domain of human IFN-gamma R2 (aa 1- 247) chain was fused to the Fc region of human IgG1 (aa 93-330). The chimeric protein was expressed in modified human 293 cells.
• Protein/Peptide Type: Biologically Active Protein
• Gene: IFNGR2

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Functional
  • Western Blot
• Application Notes: This protein is functionally active and can be used for Blocking and Neutralizing. It can also be used for Western Blot. IFN-gamma R2 - Fc HEX Chimera separates into a number of glycoforms with a pI between 5.5 and 8 on 2D PAGE due to post-translational modifications, in particular lycosylation. This compares with the unmodified IFN-gamma R2 - Fc Chimera that has a predicted pI of 7.5.

Reactivity Notes

This is a Human protein

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute with 0.5 ml sterilized PBS. After reconstitution the buffer solution will contain 1% human serum albumin and 10% trehalose already present in the vial.

Alternate Names for Recombinant Human IFN-gamma R2 Protein

• AF-1
• AF-1interferon-gamma receptor beta chain precursor10IFNGT1Interferon gamma receptor accessory factor 1
• IFGR2
• IFN-gamma R2
• IFN-gamma receptor 2
• IFNgammaR2
• IFN-gamma-R2
• IFNGR2
• IFN-gR2
• IFNGT1
• interferon gamma receptor 2 (interferon gamma transducer 1)
• interferon gamma receptor 2
• interferon gamma receptor accessory factor-1
• interferon gamma receptor beta chain
• Interferon gamma transducer 1

Background

Interferon-gamma (IFN-gamma) is a pleotropic cytokine expressed predominantly by naive and activated CD8+ and TH1 CD4+ T cells, and natural killer (NK) cells and, as such, promotes both innate and adaptive immune responses. The activity of IFN-gamma is mediated through its receptor, the high-affinity IFN-gamma receptor complex, a class II cytokine receptor that is present on T cells, B cells, macrophages, neutrophils and NK cells as well as non-immune somatic cells such as endothelial cells and fibroblasts. The high-affinity IFN-gamma receptor complex comprises two different subunits, the alpha (IFN-gamma R1, CDw119) and beta (IFN-gamma R2) subunits. The IFN-gamma R2 subunit is a 310 amino acid (~35 kDa) type I membrane protein that contains two fibronectin type III domains and six potential N-linked glycosylation sites and is involved in signal transduction. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible. Purified IFN-gamma R2 - Fc HEX Chimera consists of 20-30% carbohydrate by weight.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

Bioinformatics

• Gene Symbol: IFNGR2