IFN-gamma R2 Antibody [Alexa Fluor® 750]

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 750

IFN-gamma R2 Antibody [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IFN-γ R2
  Ser21-Val243
  Accession # NP_032364
• Specificity: Detects mouse IFN‑ gamma R2 in direct ELISAs and Western blots. In Western blots, approximately 5% cross‑reactivity with recombinant human IFN-gamma R2 is observed and less than 1% cross-reactivity with recombinant mouse (rm) IFN‑ gamma R1 and
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Neutralization
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-gamma R2 Antibody [Alexa Fluor® 750]

• AF-1
• AF-1interferon-gamma receptor beta chain precursor10IFNGT1Interferon gamma receptor accessory factor 1
• IFGR2
• IFN-gamma R2
• IFN-gamma receptor 2
• IFNgammaR2
• IFN-gamma-R2
• IFNGR2
• IFN-gR2
• IFNGT1
• interferon gamma receptor 2 (interferon gamma transducer 1)
• interferon gamma receptor 2
• interferon gamma receptor accessory factor-1
• interferon gamma receptor beta chain
• Interferon gamma transducer 1

Background

IFN‑ gamma  R2 (Interferon gamma receptor 2; also called IFN‑ gamma R beta IFN‑ gamma  RII, or AF1) is a 60‑64 kDa type I transmembrane glycoprotein that is a member of the class II cytokine receptor family of molecules (1). It is widely expressed as part of a preassembled cell surface multimeric complex. In the absence of IFN‑ gamma , the complex contains two each of IFN‑ gamma  R1, R2 and Jak1 molecules (2). Binding of IFN‑ gamma to IFN‑ gamma  R1 recruits Jak2 to IFN‑ gamma  R2 and initiates phosphorylation, STAT1 binding, conformational changes, and transcriptional regulation, which mainly inhibits proliferation and/or promotes apoptosis (2, 3). Mouse IFN‑ gamma  R2 cDNA encodes 332 amino acids (aa), including a signal sequence (aa 1‑27), an extracellular region (ECD, aa 28‑243) with two fibronectin type III domains, a transmembrane sequence (aa 244‑264) and a cytoplasmic tail (aa 265-332) (1, 2). Within the ECD, mouse IFN‑ gamma  R2 shares 80% aa sequence identity with rat IFN‑ gamma  R2, and 49‑55% with human, canine, porcine and bovine IFN‑ gamma  R2. IFN‑ gamma  R1 and R2 must be from the same species for receptor complexes to be active, and human IFN‑ gamma is not active on the mouse IFN‑ gamma receptor complex (1, 2). IFN‑ gamma  R1 is essential for ligand binding and is more constitutively expressed, while IFN‑ gamma  R2 is essential for signaling, and its more limited expression controls cell response to IFN‑ gamma (2, 3). For example, mouse T cell IFN‑ gamma  R2 is down‑regulated during differentiation to subtypes such as Th1 which produce IFN‑ gamma . (3, 4) This allows expansion of activated cells without growth arrest due to paracrine response to IFN‑ gamma . Following expansion, IFN‑ gamma  R2 is re‑expressed to limit the immune reaction (5). IFN‑ gamma  signaling mediates control of intracellular pathogens such as mycobacteria (3, 4, 6). In humans, deficiency of IFN‑ gamma  R2 or other IFN‑ gamma pathway molecules causes the MSMD (mendelian susceptibility to mycobacterial diseases) syndrome (6‑8).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.