IFN-alpha/beta R1 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IFN-alpha/beta R1 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IFN-alpha / beta R1
  Glu27-Thr429
  Accession # P33896
• Specificity: Detects mouse IFN‑ alpha / beta R1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) IFN-alpha / beta R2, rmIFN-gamma R1 and rmIFN-gamma R2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IFNAR1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-alpha/beta R1 Antibody [Biotin]

• alpha-type antiviral protein
• AVP
• beta-type antiviral protein
• CRF2-1
• Cytokine receptor class-II member 1
• Cytokine receptor family 2 member 1
• human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC
• IFN-alpha/beta R1
• IFN-alpha/beta receptor 1
• IFN-alpha-REC
• IFNAR
• IFNAR1
• IFN-aR1
• IFNBR
• IFNbR1
• IFN-bR1
• IFN-R-1
• interferon (alpha, beta and omega) receptor 1
• interferon alpha/beta receptor 1
• interferon-alpha/beta receptor alpha chain
• interferon-beta receptor 1
• Type I interferon receptor 1

Background

IFN-alpha / beta R1, also known as IFNAR1, belongs to the class II cytokine receptor family of proteins. Class II cytokine receptors form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1‑3). IFN-alpha / beta R1, in association with IFN-alpha / beta R2, is required for propagating antiviral signal transduction triggered by IFN-alpha and IFN-beta (4, 5). The mouse IFN-alpha / beta R1 cDNA encodes a 590 amino acid (aa) precursor including a 26 aa signal sequence, a 403 aa extracellular domain (ECD), a 20 aa transmembrane segment, and a 141 aa cytoplasmic domain (6). The ECD contains three tandem fibronectin type III repeats and is extensively glycosylated. The ECD of mouse IFN-alpha / beta R1 shares 47‑48% aa identity with that of human, bovine, porcine, and ovine IFN-alpha / beta R1. IFN-alpha / beta R1 interacts very weakly or not at all with type 1 interferons and does not stably interact with IFN-alpha / beta R2. Ligands associate with IFN-alpha / beta R2, and this complex subsequently forms a stable ternary assembly with IFN-alpha / beta R1 (7, 8). IFN-alpha / beta R1 also associates with IFN-gamma R2 even in the absence of IFN-gamma stimulation (5). Tyrosine phosphorylation within the juxtamembrane cytoplasmic domain of IFN-alpha / beta R1 provides a docking site for the SH2 domains of Tyk2 and STAT2 (9‑11). Tyk2 can directly phosphorylate IFN-alpha / beta R1 (10). Tyk2 also increases the level of cell surface expression of IFN-alpha / beta R1 by preventing constitutive internalization (12). Human IFN-alpha / beta R1 contains a nuclear localization signal in its ECD which is required for receptor translocation to the nucleus following interaction with ligand (13).

1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
2. Kotenko, S.V. and J.A. Langer (2004) Int. Immunopharmacol. 4:593.
3. Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
4. Hwang, S.Y. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11284.
5. Takaoka, A. et al. (2000) Science 288:2357.
6. Uze, G. et al. (1992) Proc. Natl. Acad. Sci. USA 89:4774.
7. Lamken, P. et al. (2004) J. Mol. Biol. 341:303.
8. Arduini, R.M. et al. (1999) Prot. Sci. 8:1867.
9. Krishnan, K. et al. (1998) J. Biol. Chem. 273:19495.
10. Yan, H. et al. (1996) Mol. Cell. Biol. 16:2074.
11. Yan, H. et al. (1996) EMBO J. 15:1064.
12. Ragimbeau, J. et al. (2003) EMBO J. 22:537.
13. Subramaniam, P.S. and H.M. Johnson (2004) FEBS Lett. 578:207.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IFNAR1
• Uniprot:
  • P33896()