![Western Blot: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339] - Analysis using Siha cell lysates at 1/500 dilution.](http://images.novusbio.com/fullsize/IFN-alpha-beta-R1-Antibody-SR45-08-Western-Blot-NBP2-67339-img0006.jpg "Western Blot: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339] - Analysis using Siha cell lysates at 1/500 dilution.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, IHC, IP |
| Clone | SR45-08 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit IFN-alpha/beta R1 Antibody (SR45-08) (NBP2-67339) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and IP. Anti-IFN-alpha/beta R1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human IFN-alpha/beta R1 aa 508-557 / 557. (SwissProt: P17181 Human; SwissProt: P33896 Mouse; SwissProt: D3ZDS9 Rat) |
| Localization | Cell membrane, Late endosome, Lysosome. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | IFNAR1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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| Publication using NBP2-67339 | Applications | Species |
|---|---|---|
| Agier J, Brzezinska-Blaszczyk E, Witczak P et al. The impact of TLR7 agonist R848 treatment on mast cell phenotype and activity Cellular Immunology 2020-10-01 [PMID: 33158544] (WB, FLOW, Rat) | WB, FLOW | Rat |
Secondary Antibodies |
Isotype Controls |
Research Areas for IFN-alpha/beta R1 Antibody (NBP2-67339)Find related products by research area.
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| Gene Symbol | IFNAR1 |
![Immunohistochemistry-Paraffin: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IFN-alpha/beta R1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-37, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/IFN-alpha-beta-R1-Antibody-SR45-08-Immunohistochemistry-Paraffin-NBP2-67339-img0007.jpg "Immunohistochemistry-Paraffin: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IFN-alpha/beta R1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-37, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339] - Analysis of Jurkat cells with IFNAR1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/IFN-alpha-beta-R1-Antibody-SR45-08-Flow-Cytometry-NBP2-67339-img0001.jpg "Flow Cytometry: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339] - Analysis of Jurkat cells with IFNAR1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Immunohistochemistry-Paraffin: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339] - Analysis of paraffin-embedded human spleen tissue using anti-IFNAR1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IFN-alpha-beta-R1-Antibody-SR45-08-Immunohistochemistry-Paraffin-NBP2-67339-img0002.jpg "Immunohistochemistry-Paraffin: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339] - Analysis of paraffin-embedded human spleen tissue using anti-IFNAR1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339] - Analysis of paraffin-embedded human tonsil tissue using anti-IFNAR1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IFN-alpha-beta-R1-Antibody-SR45-08-Immunohistochemistry-Paraffin-NBP2-67339-img0003.jpg "Immunohistochemistry-Paraffin: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339] - Analysis of paraffin-embedded human tonsil tissue using anti-IFNAR1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339] - Analysis of paraffin-embedded mouse brain tissue using anti-IFNAR1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IFN-alpha-beta-R1-Antibody-SR45-08-Immunohistochemistry-Paraffin-NBP2-67339-img0004.jpg "Immunohistochemistry-Paraffin: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339] - Analysis of paraffin-embedded mouse brain tissue using anti-IFNAR1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339] - Analysis of paraffin-embedded rat brain tissue using anti-IFNAR1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IFN-alpha-beta-R1-Antibody-SR45-08-Immunohistochemistry-Paraffin-NBP2-67339-img0005.jpg "Immunohistochemistry-Paraffin: IFN-alpha/beta R1 Antibody (SR45-08) [NBP2-67339] - Analysis of paraffin-embedded rat brain tissue using anti-IFNAR1 antibody. Counter stained with hematoxylin.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")