IDI1 Antibody (6G10) - Azide and BSA Free

Western Blot: IDI1 Antibody (6G10) [H00003422-M01] - Analysis of IDI1 expression in transfected 293T cell line by IDI1 monoclonal antibody (M01), clone 6G10.Lane 1: IDI1 transfected lysate(26.5 KDa).Lane 2: Non-transfected lysate.

Western Blot: IDI1 Antibody (6G10) [H00003422-M01] - IDI1 monoclonal antibody (M01), clone 6G10 Analysis of IDI1 expression in HeLa.

Sandwich ELISA: IDI1 Antibody (6G10) [H00003422-M01] - Detection limit for recombinant GST tagged IDI1 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 6G10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

IDI1 Antibody (6G10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: IDI1 (NP_004499, 175 a.a. ~ 283 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IPLEEVPPEEINYLTRIHYKAQSDGIWGEHEIDYILLVRKNVTLNPDPNEIKSYCYVSKEELKELLKKAASGEIKITPWFKIIAATFLFKWWDNLNHLNQFVDHEKIYR
• Localization: Peroxisome
• Specificity: IDI1 - isopentenyl-diphosphate delta isomerase 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IDI1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IDI1 Antibody (6G10) - Azide and BSA Free

• EC 5.3.3.2
• IPP isomerase 1
• IPP1
• IPPI1
• isopentenyl diphosphate dimethylallyl diphosphate isomerase 1
• Isopentenyl pyrophosphate isomerase 1
• isopentenyl-diphosphate delta isomerase 1
• isopentenyl-diphosphate delta isomerase
• isopentenyl-diphosphate Delta-isomerase 1

Background

IDI1 encodes a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP), which are the substrates for the successive reaction that results in the synthesis of farnesyl diphosphate and, ultimately, cholesterol. It has been shown in peroxisomal deficiency diseases such as Zellweger syndrome and neonatal adrenoleukodystrophy that there is reduction in IPP isomerase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IDI1
• Entrez:
  • 3422(Human)
• OMIM:
  • 604055(Human)
• Uniprot:
  • NP_004499(Human)