IDH3B Antibody

Western Blot: IDH3B Antibody [NBP1-78787] - Staining of Pig Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: IDH3B Antibody [NBP1-78787] (0.3ug/ml) staining of Human Skeletal muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: IDH3B Antibody [NBP1-78787] - Staining of Mouse Heart (A) and Mouse Muscle (B) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

IDH3B Antibody Summary

• Description: Novus Biologicals Goat IDH3B Antibody (NBP1-78787) is a polyclonal antibody validated for use in WB and ELISA. Anti-IDH3B Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HAASRSQAEDVRVE corresponding to internal region (near N-Terminus) according to NP_777280.1.
• Specificity: This antibody is expected to recognize isoform a (NP_008830.2) and isoform b (NP_777280.1).
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IDH3B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 39 kDa band observed in mouse, rat and porcine heart lysates and in human and mouse skeletal muscle lysates (calculated MW of 41.9 kDa band according to NP_777280.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IDH3B Antibody

• EC 1.1.1
• EC 1.1.1.41
• FLJ11043
• H-IDHB
• isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
• isocitrate dehydrogenase 3 (NAD+) beta
• isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, beta subunit
• Isocitric dehydrogenase subunit beta
• MGC903
• NAD(+)-specific ICDH subunit beta
• NAD+-specific ICDH
• NAD+-specific isocitrate dehydrogenase b subunit
• NAD+-specific isocitrate dehydrogenase beta
• RP46

Background

IDH3B codes for a protein that has three isoforms, with lengths of 385, 383, and 233 amino acids, and weights of 42, 42, and 26 kDa respectively, and is a protein that is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase, which localizes to the mitochondrial matrix. Current studies are being done on several diseases and disorders related to this gene, including retinitis pigmentosa, blindness, and ataxia. IDH3B has also been shown to have interactions with MAPK6, MYC, IDH3G, PHLDA3, and IDH3A in pathways such as the TCA cycle and metabolic pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IDH3B Antibody (NBP1-78787)(1)

Publication using NBP1-78787

• Bzymek KP, Colman RF. Role of alpha-Asp181, beta-Asp192, and gamma-Asp190 in the distinctive subunits of human NAD-specific isocitrate dehydrogenase. Biochemistry 2007-05-08 [PMID: 17432878]

Bioinformatics

• Gene Symbol: IDH3B
• Entrez:
  • 3420(Human)
  • 170718(Mouse)
  • 94173(Rat)
• Uniprot:
  • NP_008830.2(Human)