IDH2 Antibody (JA55-31)

Western Blot: IDH2 Antibody (JA55-31) [NBP2-66899] - Analysis of IDH2 on different lysates using anti-IDH2 antibody at 1/1,000 dilution. Positive control:Line 1: Mouse heart Line 2: Rat heart Line 3: 293T

Immunohistochemistry-Paraffin: IDH2 Antibody (JA55-31) [NBP2-66899] - Analysis of paraffin-embedded rat heart tissue using anti-IDH2 antibody. Counter stained with hematoxylin.

Flow Cytometry: IDH2 Antibody (JA55-31) [NBP2-66899] - Analysis of MCF-7 cells with IDH2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunohistochemistry-Paraffin: IDH2 Antibody (JA55-31) [NBP2-66899] - Analysis of paraffin-embedded human liver tissue using anti-IDH2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: IDH2 Antibody (JA55-31) [NBP2-66899] - Analysis of paraffin-embedded human prostate tissue using anti-IDH2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: IDH2 Antibody (JA55-31) [NBP2-66899] - Analysis of paraffin-embedded human tonsil tissue using anti-IDH2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: IDH2 Antibody (JA55-31) [NBP2-66899] - Analysis of paraffin-embedded mouse heart tissue using anti-IDH2 antibody. Counter stained with hematoxylin.

Product Discontinued

IDH2 Antibody (JA55-31) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human IDH2 aa 403-452 / 452. (SwissProt: P48735 Human; SwissProt: P54071 Mouse; SwissProt: P56574 Rat)
• Localization: Mitochondrion.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IDH2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IDH2 Antibody (JA55-31)

• D2HGA2
• EC 1.1.1.42
• ICD-M
• IDH
• IDHM
• IDP
• IDPM
• isocitrate dehydrogenase [NADP], mitochondrial
• isocitrate dehydrogenase 2 (NADP+), mitochondrial
• mNADP-IDH
• NADP(+)-specific ICDH
• Oxalosuccinate decarboxylase

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IDH2