IBTK Antibody

Western Blot: IBTK Antibody [NBP1-50033] - Detection of Human IBtk by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-IBtk antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IBtk was also immunoprecipitated by rabbit anti-IBtk antibody BL10880, which recognizes an upstream epitope. For blotting immunoprecipitated IBtk, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

IBTK Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 1050 and 1100 of human Inhibitor of Bruton Agammaglobulinemia Tyrosine Kinase, using the numbering given in entry NP_056340.2 (GeneID 25998).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IBTK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 mcg/mg
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IBTK Antibody

• Bruton agammaglobulinemia tyrosine kinase inhibitor
• BTK-binding protein
• BTKI
• DKFZP564B116
• IBtk
• inhibitor of Bruton agammaglobulinemia tyrosine kinase
• inhibitor of Bruton tyrosine kinase
• inhibitor of Bruton's tyrosine kinase
• inhibitor of Bruton's tyrosine kinase-alpha
• inhibitor of Bruton's tyrosine kinase-beta
• inhibitor of Bruton's tyrosine kinase-gamma
• KIAA1417
• MGC142256
• MGC142258
• RP1-93K22.1

Background

The protein encoded by this gene binds to Bruton's tyrosine kinase (BTK) and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IBTK Antibody (NBP1-50033)(1)

Publication using NBP1-50033

• Willy JA, Young SK, Mosley AL et al. Function of Inhibitor of Brutons Tyrosine Kinase Isoform alpha (IBTKalpha) in Nonalcoholic Steatohepatitis links Autophagy and the Unfolded Protein Response J. Biol. Chem. 2017-07-14 [PMID: 28710282] (WB, Human)

Bioinformatics

• Gene Symbol: IBTK