Human WISP-1/CCN4 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human WISP-1/CCN4 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: WISP1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human WISP-1/CCN4 DuoSet ELISA, 15 Plate

• CCN family member 4
• CCN4
• CCN4WISP1tc
• WISP1
• WISP-1
• WISP1c
• WISP1i
• WNT1 induced secreted protein 1
• WNT1 inducible signaling pathway protein 1
• Wnt-1 inducible signaling pathway protein 1
• wnt-1 signaling pathway protein 1
• Wnt-1-induced secreted protein
• WNT1-inducible-signaling pathway protein 1

Background

WISP-1 belongs to the CCN (CTGF/CYR61/NOV) family of secreted proteins that share a common multimodular organization. Each family member has an IGFBP domain, a von Willebrand type C domain, a thrombospondin 1 domain and a C-terminal cysteine knot domain. Depending on cell context, WISP-1 can either promote or inhibit tumorigenesis.

Publications for WISP-1/CCN4 (DY1627)(5)

Publications using DY1627

• DJ Klinke Ii, A Fernandez, W Deng, A Razazan, H Latifizade, AC Pirkey Data-driven learning how oncogenic gene expression locally alters heterocellular networks Nature Communications, 2022-04-13;13(1):1986. 2022-04-13 [PMID: 35418177] (Mouse)
• O Pivovarova, J Loske, S Hornemann, M Markova, N Seebeck, A Rosenthal, F Klauschen, JP Castro, R Buschow, T Grune, V Lange, N Rudovich, DM Ouwens Hepatic Wnt1 Inducible Signaling Pathway Protein 1 (WISP-1/CCN4) Associates with Markers of Liver Fibrosis in Severe Obesity Cells, 2021-04-29;10(5):. 2021-04-29 [PMID: 33946738] (Human)
• H Shailesh, KS Siveen, S Sif Protein arginine methyltransferase 5 (PRMT5) activates WNT/&beta-catenin signalling in breast cancer cells via epigenetic silencing of DKK1 and DKK3 Journal of Cellular and Molecular Medicine, 2021-01-18;0(0):. 2021-01-18 [PMID: 33462997] (Human)
• W Deng, A Fernandez, SL McLaughlin, DJ Klinke WNT1-inducible signaling pathway protein 1 (WISP1/CCN4) stimulates melanoma invasion and metastasis by promoting the epithelial - mesenchymal transition J. Biol. Chem., 2019-02-05;0(0):. 2019-02-05 [PMID: 30723155] (Human)
• Li , Wen-Feng, Zhang , Li, Li , Hai-Ying, Zheng , Si-Si, Zhao , Liang WISP-1 contributes to fractionated irradiation-induced radioresistance in esophageal carcinoma cell lines and mice. PLoS ONE, 2014-04-11;9(4):e94751. 2014-04-11 [PMID: 24728101] (Human)

FAQs for WISP-1/CCN4 (DY1627). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: WISP1