Human u-Plasminogen Activator/Urokinase Quantikine ELISA

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human u-Plasminogen Activator/Urokinase Quantikine ELISA Summary

• Background: The Quantikine Human u-Plasminogen Activator/Urokinase Immunoassay is a 4.5 hour solid phase ELISA designed to measure uPA in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant human uPA and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human uPA. Results obtained usin...g natural human uPA showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human uPA.
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• Specificity: Natural and recombinant human uPA. This assay also recognizes uPA complexed with PAI-1 or uPAR.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: PLAU

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human u-Plasminogen Activator/Urokinase Quantikine ELISA

• ATF
• EC 3.4.21
• EC 3.4.21.73
• plasminogen activator, urokinase
• PLAU
• uPA
• u-PA
• uPlasminogen Activator
• u-Plasminogen Activator
• urinary
• Urokinase
• urokinase-type plasminogen activator

Background

u-Plasminogen Activator (uPA) is a serine protease that converts plasminogen to plasmin, with roles in a variety of normal and pathological processes that include cell migration and tissue destruction. uPA is a potent marker of invasion and metastasis in a variety of human cancers including breast, stomach, colon, bladder, ovarian, brain, and endometrium.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for u-Plasminogen Activator/Urokinase (DUPA00)(5)

Publications using DUPA00

• Y Cheng, TR Hall, X Xu, I Yung, D Souza, J Zheng, F Schiele, M Hoffmann, ML Mbow, JP Garnett, J Li Targeting uPA-uPAR interaction to improve intestinal epithelial barrier integrity in inflammatory bowel disease EBioMedicine, 2021-12-18;75(0):103758. 2021-12-18 [PMID: 34933179] (Human)
• S Durlanik, K Fundel-Cle, C Viollet, HJ Huber, M Lenter, K Kitt, S Pflanz CD276 is an important player in macrophage recruitment into the tumor and an upstream regulator for PAI-1 Scientific Reports, 2021-07-21;11(1):14849. 2021-07-21 [PMID: 34290311] (Human)
• Chang M, Chang H, Chan C, Yeung S, Hsien H, Lin B, Yeh C, Tseng W, Tseng S, Jeng J p-Cresol affects reactive oxygen species generation, cell cycle arrest, cytotoxicity and inflammation/atherosclerosis-related modulators production in endothelial cells and mononuclear cells. PLoS ONE, 2014-12-17;9(12):e114446. 2014-12-17 [PMID: 25517907] (Human)
• Karagiannis G, Schaeffer D, Cho C, Musrap N, Saraon P, Batruch I, Grin A, Mitrovic B, Kirsch R, Riddell R, Diamandis E Collective migration of cancer-associated fibroblasts is enhanced by overexpression of tight junction-associated proteins claudin-11 and occludin. Mol Oncol, 2013-11-08;8(2):178-95. 2013-11-08 [PMID: 24268521] (Human)
• Scheel C, Eaton E, Li S, Chaffer C, Reinhardt F, Kah K, Bell G, Guo W, Rubin J, Richardson A, Weinberg R Paracrine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast. Cell, 2011-06-10;145(6):926-40. 2011-06-10 [PMID: 21663795] (Human)

FAQs for u-Plasminogen Activator/Urokinase (DUPA00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PLAU