Human u-Plasminogen Activator/Urokinase Quantikine ELISA

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human u-Plasminogen Activator/Urokinase Quantikine ELISA Summary

• Background: The Quantikine Human u-Plasminogen Activator/Urokinase Immunoassay is a 4.5 hour solid phase ELISA designed to measure uPA in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant human uPA and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human uPA. Results obtained usin...g natural human uPA showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human uPA.
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• Specificity: Natural and recombinant human uPA. This assay also recognizes uPA complexed with PAI-1 or uPAR.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: PLAU

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human u-Plasminogen Activator/Urokinase Quantikine ELISA

• ATF
• EC 3.4.21
• EC 3.4.21.73
• plasminogen activator, urokinase
• PLAU
• uPA
• u-PA
• uPlasminogen Activator
• u-Plasminogen Activator
• urinary
• Urokinase
• urokinase-type plasminogen activator

Background

u-Plasminogen Activator (uPA) is a serine protease that converts plasminogen to plasmin, with roles in a variety of normal and pathological processes that include cell migration and tissue destruction. uPA is a potent marker of invasion and metastasis in a variety of human cancers including breast, stomach, colon, bladder, ovarian, brain, and endometrium.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for u-Plasminogen Activator/Urokinase (DUPA00)(5)

Publications using DUPA00

• Y Cheng, TR Hall, X Xu, I Yung, D Souza, J Zheng, F Schiele, M Hoffmann, ML Mbow, JP Garnett, J Li Targeting uPA-uPAR interaction to improve intestinal epithelial barrier integrity in inflammatory bowel disease EBioMedicine, 2021-12-18;75(0):103758. 2021-12-18 [PMID: 34933179] (Human)
• S Durlanik, K Fundel-Cle, C Viollet, HJ Huber, M Lenter, K Kitt, S Pflanz CD276 is an important player in macrophage recruitment into the tumor and an upstream regulator for PAI-1 Scientific Reports, 2021-07-21;11(1):14849. 2021-07-21 [PMID: 34290311] (Human)
• Chang M, Chang H, Chan C, Yeung S, Hsien H, Lin B, Yeh C, Tseng W, Tseng S, Jeng J p-Cresol affects reactive oxygen species generation, cell cycle arrest, cytotoxicity and inflammation/atherosclerosis-related modulators production in endothelial cells and mononuclear cells. PLoS ONE, 2014-12-17;9(12):e114446. 2014-12-17 [PMID: 25517907] (Human)
• Karagiannis G, Schaeffer D, Cho C, Musrap N, Saraon P, Batruch I, Grin A, Mitrovic B, Kirsch R, Riddell R, Diamandis E Collective migration of cancer-associated fibroblasts is enhanced by overexpression of tight junction-associated proteins claudin-11 and occludin. Mol Oncol, 2013-11-08;8(2):178-95. 2013-11-08 [PMID: 24268521] (Human)
• Scheel C, Eaton E, Li S, Chaffer C, Reinhardt F, Kah K, Bell G, Guo W, Rubin J, Richardson A, Weinberg R Paracrine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast. Cell, 2011-06-10;145(6):926-40. 2011-06-10 [PMID: 21663795] (Human)

Bioinformatics

• Gene Symbol: PLAU