Human TSLP Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human TSLP Quantikine ELISA Kit Summary

• Background: The Quantikine Human TSLP immunoassay is a 4.5 hour sandwich-type solid phase ELISA designed to measure human TSLP in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human TSLP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TSLP showed linear curves that were parallel to the standard curv...es obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human TSLP.
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• Specificity: Natural and recombinant human TSLP
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: TSLP

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TSLP Quantikine ELISA Kit

• thymic stromal lymphopoietin
• TSLP

Background

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220⁺/IgM^- pre-B cells, mouse TSLP promotes the development B220⁺/IgM⁺ B cells. Human TSLP was reported to preferentially stimulate myeloid cells, inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c⁺ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence. Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to Rgamma_c.

TSLP R, also named Delta and CRLM-2 (Cytokine Receptor-like Module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain (gamma c). It was subsequently identified as a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R. TSLP R shows 24% sequence identity with the mouse gc receptor. TSLP R expression is constitutive in immune and hematopoietic cells, but is up-regulated in Th2 cells.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for TSLP (DTSLP0)(17)

Publications using DTSLP0

• Hsieh, YA;Hsiao, YH;Ko, HK;Shen, YL;Huang, CW;Perng, DW;Su, KC; House dust mites stimulate thymic stromal lymphopoietin production in human bronchial epithelial cells and promote airway remodeling through activation of PAR2 and ERK signaling pathway Scientific reports 2024-11-19 [PMID: 39562597] (Human)
• Weng, CM;Lee, MJ;Chao, W;Lin, YR;Chou, CJ;Chen, MC;Chou, CL;Lin Tsai, I;Lin, CH;Fan Chung, K;Kuo, HP; Airway epithelium IgE-Fc?RI Cross-Link Induces Epithelial Barrier Disruption in Severe T2-high Asthma Mucosal immunology 2023-08-01 [PMID: 37536562] (Human)
• Z Shen, W Luo, B Tan, K Nie, M Deng, S Wu, M Xiao, X Wu, X Meng, T Tong, C Zhang, K Ma, Y Liao, J Xu, X Wang Roseburia intestinalis stimulates TLR5-dependent intestinal immunity against Crohn's disease EBioMedicine, 2022-09-28;85(0):104285. 2022-09-28 [PMID: 36182776] (Human)
• SZ Li, XX Jin, XL Ge, YG Zuo, HZ Jin Thymic Stromal Lymphopoietin Is Implicated in the Pathogenesis of Bullous Pemphigoid by Dendritic Cells J Immunol Res, 2020-09-24;2020(0):4594630. 2020-09-24 [PMID: 33029540] (Human)
• G Anzalone, GD Albano, AM Montalbano, L Riccobono, A Bonanno, R Gagliardo, F Bucchieri, R Marchese, M Moscato, M Profita IL-17A-associated IKK-? signaling induced TSLP production in epithelial cells of COPD patients Exp. Mol. Med., 2018-10-05;50(10):131. 2018-10-05 [PMID: 30291224] (Human)
• HL Yang, KK Chang, J Mei, WJ Zhou, LB Liu, L Yao, Y Meng, MY Wang, SY Ha, ZZ Lai, JF Ye, DJ Li, MQ Li Estrogen restricts the apoptosis of endometrial stromal cells by promoting TSLP secretion Mol Med Rep, 2018-08-24;0(0):. 2018-08-24 [PMID: 30152851]
• YA Tyurin, SA Lissovskay, RS Fassahov, IG Mustafin, AF Shamsutdin, MA Shilova, AA Rizvanov Cytokine Profile of Patients with Allergic Rhinitis Caused by Pollen, Mite, and Microbial Allergen Sensitization J Immunol Res, 2017-10-04;2017(0):3054217. 2017-10-04 [PMID: 29109963] (Human)
• Wei Zhang Betamethasone Butyrate Propionate Inhibits the Induction of Thymic Stromal Lymphopoietin in Cultured Normal Human Keratinocytes Ann Dermatol, 2016-11-23;28(6):772-775. 2016-11-23 [PMID: 27904282] (Human)
• Danso M, van Drongelen V, Mulder A, van Esch J, Scott H, van Smeden J, El Ghalbzouri A, Bouwstra J TNF-alpha and Th2 cytokines induce atopic dermatitis-like features on epidermal differentiation proteins and stratum corneum lipids in human skin equivalents. J Invest Dermatol, 2014-02-11;134(7):1941-50. 2014-02-11 [PMID: 24518171] (Human)
• Bleck B, Grunig G, Chiu A, Liu M, Gordon T, Kazeros A, Reibman J MicroRNA-375 regulation of thymic stromal lymphopoietin by diesel exhaust particles and ambient particulate matter in human bronchial epithelial cells. J Immunol, 2013-03-01;190(7):3757-63. 2013-03-01 [PMID: 23455502] (Human)
• Fontao L, Laffitte E, Briot A, Kaya G, Roux-Lombard P, Fraitag S, Hovnanian AA, Saurat JH Infliximab infusions for netherton syndrome: sustained clinical improvement correlates with a reduction of thymic stromal lymphopoietin levels in the skin. J. Invest. Dermatol., 2011-06-09;131(9):1947-50. 2011-06-09 [PMID: 21654837] (Human)
• Semlali A, Jacques E, Koussih L, Gounni AS, Chakir J Thymic stromal lymphopoietin-induced human asthmatic airway epithelial cell proliferation through an IL-13-dependent pathway. J. Allergy Clin. Immunol., 2010-03-17;125(4):844-50. 2010-03-17 [PMID: 20236697] (Human)
• Zheng X, Ma P, De Paiva CS, Cunningham MA, Hwang CS, Pflugfelder SC, Li DQ TSLP and downstream molecules in experimental mouse allergic conjunctivitis. Invest. Ophthalmol. Vis. Sci., 2010-01-27;51(6):3076-82. 2010-01-27 [PMID: 20107175] (Human)
• Xu G, Zhang L, Wang DY, Xu R, Liu Z, Han DM, Wang XD, Zuo KJ, Li HB Opposing roles of IL-17A and IL-25 in the regulation of TSLP production in human nasal epithelial cells. Allergy, 2009-11-25;65(5):581-9. 2009-11-25 [PMID: 19968632] (Human)
• Le TA, Takai T, Kinoshita H, Suto H, Ikeda S, Okumura K, Ogawa H Inhibition of double-stranded RNA-induced TSLP in human keratinocytes by glucocorticoids. Allergy, 2009-03-27;64(8):1231-2. 2009-03-27 [PMID: 19416141] (Human)
• Ma P, Bian F, Wang Z, Zheng X, Chotikavanich S, Pflugfelder SC, Li DQ Human corneal epithelium-derived thymic stromal lymphopoietin links the innate and adaptive immune responses via TLRs and Th2 cytokines. Invest. Ophthalmol. Vis. Sci., 2009-01-17;50(6):2702-9. 2009-01-17 [PMID: 19151401] (Human)
• Versluis M, van den Berge M, Timens W, Luijk B, Rutgers B, Lammers JW, Postma DS, Hylkema MN Allergen inhalation decreases adenosine receptor expression in sputum and blood of asthma patients. Allergy, 2008-09-01;63(9):1186-94. 2008-09-01 [PMID: 18699935] (Human)

FAQs for TSLP (DTSLP0). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TSLP