Human TSLP DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human TSLP DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: TSLP

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TSLP DuoSet ELISA, 15 Plate

• thymic stromal lymphopoietin
• TSLP

Background

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220⁺/IgM^- pre-B cells, mouse TSLP promotes the development B220⁺/IgM⁺ B cells. Human TSLP was reported to preferentially stimulate myeloid cells, inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c⁺ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence. Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to Rgamma_c.

TSLP R, also named Delta and CRLM-2 (Cytokine Receptor-like Module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain (gamma c). It was subsequently identified as a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R. TSLP R shows 24% sequence identity with the mouse gc receptor. TSLP R expression is constitutive in immune and hematopoietic cells, but is up-regulated in Th2 cells.

Publications for TSLP (DY1398)(12)

Publications using DY1398

• Marcella, S;Braile, M;Grimaldi, AM;Soricelli, A;Smaldone, G; Exploring thymic stromal lymphopoietin in the breast cancer microenvironment: A preliminary study Oncology letters 2025-04-01 [PMID: 40007626] (Human)
• Yu, H;Zhang, C;Pan, H;Gao, X;Wang, X;Xiao, W;Yan, S;Gao, Y;Fu, J;Zhou, Y; Indeno[1,2,3-cd]pyrene enhances the sensitivity of airway epithelial cells to ferroptosis and aggravates asthma Chemosphere 2024-07-16 [PMID: 39025314] (Mouse)
• De Monte, L;Clemente, F;Ruggiero, E;Pini, R;Ceraolo, MG;Schiavo Lena, M;Balestrieri, C;Lazarevic, D;Belfiori, G;Crippa, S;Balzano, G;Falconi, M;Doglioni, C;Bonini, C;Reni, M;Protti, MP; Pro-tumor Tfh2 cells induce detrimental IgG4 production and PGE2-dependent IgE inhibition in pancreatic cancer EBioMedicine 2023-09-28 [PMID: 37776595] (Human)
• J Kim, K Hegener, C Hagedorn, K Jamal Jame, D Weidinger, IMC Seuthe, S Eichhorn, F Kreppel, J Knobloch, JJ Park Long-Term Cryopreservation of Nasal Polyp Tissue in a Biobank for the Isolation and Culture of Primary Epithelial Cells International Journal of Molecular Sciences, 2023-03-28;24(7):. 2023-03-28 [PMID: 37047354] (Human)
• Q Yu, Y Li, H Wang, H Xiong TSLP induces a proinflammatory phenotype in circulating innate cells and predicts prognosis in sepsis patients FEBS Open Bio, 2019-12-01;0(0):. 2019-12-01 [PMID: 31628890] (Human)
• K Verstraete, F Peelman, H Braun, J Lopez, D Van Rompae, A Dansercoer, I Vandenberg, K Pauwels, J Tavernier, BN Lambrecht, H Hammad, H De Winter, R Beyaert, G Lippens, SN Savvides Structure and antagonism of the receptor complex mediated by human TSLP in allergy and asthma Nat Commun, 2017-04-03;8(0):14937. 2017-04-03 [PMID: 28368013] (Human)
• Byung Min Chung, Artem Arutyunov, Erika Ilagan, Nu Yao, Marsha Wills-Karp, Pierre A. Coulombe Regulation of C-X-C chemokine gene expression by keratin 17 and hnRNP K in skin tumor keratinocytes Journal of Cell Biology 2015-03-02 [PMID: 25713416] (Human)
• Hui C, Yu A, Heroux D, Akhabir L, Sandford A, Neighbour H, Denburg J Thymic stromal lymphopoietin (TSLP) secretion from human nasal epithelium is a function of TSLP genotype. Mucosal Immunol, 2014-12-17;8(5):993-9. 2014-12-17 [PMID: 25515628] (Human)
• Interleukin-1alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33. J. Exp. Med., 2012-07-16;209(8):1505-17. 2012-07-16 [PMID: 22802353] (Human)
• Okayama Y, Okumura S, Sagara H, Yuki K, Sasaki T, Watanabe N, Fueki M, Sugiyama K, Takeda K, Fukuda T, Saito H, Ra C FcepsilonRI-mediated thymic stromal lymphopoietin production by interleukin-4-primed human mast cells. Eur. Respir. J., 2009-01-22;34(2):425-35. 2009-01-22 [PMID: 19164348] (Human)
• Kinoshita H, Takai T, Le TA, Kamijo S, Wang XL, Ushio H, Hara M, Kawasaki J, Vu AT, Ogawa T, Gunawan H, Ikeda S, Okumura K, Ogawa H Cytokine milieu modulates release of thymic stromal lymphopoietin from human keratinocytes stimulated with double-stranded RNA. J. Allergy Clin. Immunol., 2008-12-03;123(1):179-86. 2008-12-03 [PMID: 19056108] (Human)
• Kato A, Favoreto S, Avila PC, Schleimer RP TLR3- and Th2 cytokine-dependent production of thymic stromal lymphopoietin in human airway epithelial cells. J. Immunol., 2007-07-15;179(2):1080-7. 2007-07-15 [PMID: 17617600] (Human)

Review for TSLP (DY1398) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Patricia Carulla Martí 06/22/2023

Summary

• Sample Tested: Cultured Human Keratinocytes,Supernatant Human Epidermal keratinocytes (HEKa) in presence or absence of Poly(I:C)
• Lot: P305031

FAQs for TSLP (DY1398). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TSLP