Human Total IL-18/IL-1F4 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Total IL-18/IL-1F4 Quantikine ELISA Kit Summary

• Background: The Quantikine® Human Total IL-18/IL-1F4 Immunoassay is a 4.0 hour solid-phase ELISA designed to measure human IL-18 in cell culture supernates, serum, plasma, saliva, and urine. It contains E. coli-expressed recombinant human IL-18 and has been shown to accurately quantitate the recombinant factor free and in complex with IL-18 BPa. Results obtained using natural human IL-18 showed linear ...curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-18.
  Show More
• Specificity: Natural and recombinant human Total IL-18.
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Total IL-18/IL-1F4 Quantikine ELISA Kit

• Iboctadekin
• IFN-gamma-inducing factor
• IGIF
• IGIFIL-1 gamma
• IL18
• IL-18
• IL-18MGC12320
• IL-1F4
• IL1F4iboctadekin
• IL-1g
• Interferon gamma-inducing factor
• interleukin 18 (interferon-gamma-inducing factor)
• Interleukin-1 gamma
• interleukin-18

Background

Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δT cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases. IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen independent production of IL-17 by gamma δ T cells and CD4+ T cells (11). IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature human IL-18 shares approximately 63% amino acid sequence identity with mouse and rat IL-18 (15). Alternative splicing in human ovarian cancer generates an isoform that is resistant to Caspase-1 activation (16). A cell surface form can be expressed on M-CSF induced macrophages and released in response to bacterial endotoxin (17).

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-18/IL-1F4 (DL180)(25)

Publications using DL180

• Martinez Valenzuela, L;Vidal-Alabró, A;Rubio, B;Antón-Pàmpols, P;Gómez-Preciado, F;Fulladosa, X;Cruzado, JM;Torras, J;Lloberas, N;Draibe, J; Evaluating Single-Nucleotide Polymorphisms in Inflammasome Proteins and Serum Levels of IL-18 and IL-1? in Kidney Interstitial Damage in Anti-Neutrophilic Cytoplasmic Antibody-Associated Vasculitis International journal of molecular sciences 2024-06-12 [PMID: 38928186] (Human)
• Neubauer-Geryk, J;Wielicka, M;My?liwiec, M;Zorena, K;Bieniaszewski, L; The Impact of Metabolic Memory on Immune Profile in Young Patients with Uncomplicated Type 1 Diabetes International journal of molecular sciences 2024-03-10 [PMID: 38542165] (Human)
• Kosmider, O;Possémé, C;Templé, M;Corneau, A;Carbone, F;Duroyon, E;Breillat, P;Chirayath, TW;Oules, B;Sohier, P;Luka, M;Gobeaux, C;Lazaro, E;Outh, R;Le Guenno, G;Lifermann, F;Berleur, M;Le Mene, M;Friedrich, C;Lenormand, C;Weitten, T;Guillotin, V;Burroni, B;Boussier, J;Willems, L;Aractingi, S;Dionet, L;Tharaux, PL;Vergier, B;Raynaud, P;Ea, HK;Ménager, M;Duffy, D;Terrier, B; VEXAS syndrome is characterized by inflammasome activation and monocyte dysregulation Nature communications 2024-01-30 [PMID: 38291039] (Human)
• Exconde, PM;Hernandez-Chavez, C;Bourne, CM;Richards, RM;Bray, MB;Lopez, JL;Srivastava, T;Egan, MS;Zhang, J;Yoo, W;Shin, S;Discher, BM;Taabazuing, CY; The tetrapeptide sequence of IL-18 and IL-1? regulates their recruitment and activation by inflammatory caspases Cell reports 2023-12-15 [PMID: 38103201] (Human)
• Musia?, K;Stojanowski, J;Mi?kiewicz-Bujna, J;Ka?wak, K;Ussowicz, M; KIM-1, IL-18, and NGAL, in the Machine Learning Prediction of Kidney Injury among Children Undergoing Hematopoietic Stem Cell Transplantation-A Pilot Study International journal of molecular sciences 2023-10-31 [PMID: 37958774] (Human)
• Nabatanzi, R;Ssekamatte, P;Castelnuovo, B;Kambugu, A;Nakanjako, D; Increased Levels of Caspase-1 and IL-1? Among Adults With Persistent Immune Activation After 12 Years of Suppressive Antiretroviral Therapy in the Infectious Diseases Institute HIV Treatment Cohort Open forum infectious diseases 2023-11-01 [PMID: 37953818] (Human)
• SR Schnittman, DW Kitch, TH Swartz, TH Burdo, KV Fitch, S McCallum, JM Flynn, ES Fulda, MR Diggs, JT Stapleton, JL Casado, J Taron, JS Currier, MV Zanni, C Malvestutt, CJ Fichtenbau, JA Aberg, HJ Ribaudo, MT Lu, PS Douglas, SK Grinspoon Coronary Artery Plaque Composition and Severity Relate to the Inflammasome in People With Treated Human Immunodeficiency Virus Open forum infectious diseases, 2023-03-01;10(3):ofad106. 2023-03-01 [PMID: 36998633] (Human)
• LH Willems, LMC Jacobs, LA Groh, H Ten Cate, HMH Spronk, B Wilson-Sto, G Hannink, SMJ van Kuijk, C Ghossein-D, M Nagy, DHJ Thijssen, AS van Peters, MC Warlé Vascular Function, Systemic Inflammation, and Coagulation Activation 18 Months after COVID-19 Infection: An Observational Cohort Study Journal of Clinical Medicine, 2023-02-10;12(4):. 2023-02-10 [PMID: 36835948] (Human)
• J Milara, F Martínez-E, P Montero, I Roger, MA Bayarri, P Ribera, MN Oishi-Kona, JR Alba-Garcí, E Zapater, J Cortijo N-acetylcysteine Reduces Inflammasome Activation Induced by SARS-CoV-2 Proteins In Vitro International Journal of Molecular Sciences, 2022-11-22;23(23):. 2022-11-22 [PMID: 36498845] (Human)
• P Szczepanik, M Michalska-, M Kowal, D Krasowska Serum Levels of Selected IL-1 Family Cytokines in Patients with Morphea Journal of Clinical Medicine, 2022-10-28;11(21):. 2022-10-28 [PMID: 36362603] (Human)
• MC Chiang, CJB Nicol, SS Lo, SW Hung, CJ Wang, CH Lin Resveratrol Mitigates Oxygen and Glucose Deprivation-Induced Inflammation, NLRP3 Inflammasome, and Oxidative Stress in 3D Neuronal Culture International Journal of Molecular Sciences, 2022-10-02;23(19):. 2022-10-02 [PMID: 36232980] (Human)
• F Piancone, F La Rosa, I Marventano, A Hernis, R Miglioli, F Trecate, M Saresella, M Clerici Modulation of Neuroendocrine and Immunological Biomarkers Following Rehabilitation in Sarcopenic Patients Cells, 2022-08-10;11(16):. 2022-08-10 [PMID: 36010554] (Human)
• F La Rosa, CP Zoia, C Bazzini, A Bolognini, M Saresella, E Conti, C Ferrarese, F Piancone, I Marventano, D Galimberti, C Fenoglio, E Scarpini, M Clerici Modulation of MAPK- and PI3/AKT-Dependent Autophagy Signaling by Stavudine (D4T) in PBMC of Alzheimer's Disease Patients Cells, 2022-07-12;11(14):. 2022-07-12 [PMID: 35883623] (Human)
• DB Rosen, EA Murphy, RS Gejman, A Capili, RL Friedlande, S Rand, KA Cagino, SM Glynn, KC Matthews, JM Kubiak, J Yee, M Prabhu, LE Riley, YJ Yang Cytokine response over the course of COVID-19 infection in pregnant women Cytokine, 2022-04-25;154(0):155894. 2022-04-25 [PMID: 35490452] (Human)
• Tominaga T, Ikemura T, Yada K, Kanda K, Sugama K, Ma S, Choi W, Araya M, Huang J, Nakamura N, Suzuki K The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males. Antioxidants (Basel), 2021-05-28;10(6):. 2021-05-28 [PMID: 34071378] (Human)
• F La Rosa, S Agostini, M Saresella, AS Costa, F Piancone, R Miglioli, F Trecate, M Clerici Deregulation of IL-37 and its miRNAs modulators in sarcopenic patients after rehabilitation Journal of Translational Medicine, 2021-04-26;19(1):172. 2021-04-26 [PMID: 33902634] (Human)
• ML Matias, M Romao-Veig, VR Ribeiro, PR Nunes, VJ Gomes, AC Devides, VT Borges, GG Romagnoli, JC Peracoli, MT Peracoli Progesterone and vitamin D downregulate the activation of the NLRP1/NLRP3 inflammasomes and TLR4-MyD88-NF-&kappaB pathway in monocytes from pregnant women with preeclampsia Journal of reproductive immunology, 2021-02-05;144(0):103286. 2021-02-05 [PMID: 33578174] (Human)
• DM de Graaf, M Jaeger, ICL van den Mu, RT Horst, K Schraa, J Zwaag, M Kox, M Fujita, T Yamauchi, L Mercurio, S Madonna, JHW Rutten, J de Graaf, NP Riksen, FL van de Vee, MG Netea, LAB Joosten, CA Dinarello Reduced concentrations of the B cell cytokine Interleukin 38 are associated with cardiovascular disease risk in overweight subjects Eur J Immunol, 2020-11-19;0(0):. 2020-11-19 [PMID: 33125159] (Human)
• H Tsugawa, Y Kabe, A Kanai, Y Sugiura, S Hida, S Taniguchi, T Takahashi, H Matsui, Z Yasukawa, H Itou, K Takubo, H Suzuki, K Honda, H Handa, M Suematsu Short-chain fatty acids bind to apoptosis-associated speck-like protein to activate inflammasome complex to prevent Salmonella infection PLoS Biol, 2020-09-29;18(9):e3000813. 2020-09-29 [PMID: 32991574] (Mouse)
• FS Thaler, B Bangol, M Biljecki, J Havla, AM Schumacher, T Kümpfel Possible link of genetic variants to autoimmunity in GAD-antibody-associated neurological disorders J. Neurol. Sci., 2020-04-29;413(0):116860. 2020-04-29 [PMID: 32388243] (Human)
• SR Kim, SG Lee, SH Kim, JH Kim, E Choi, W Cho, JH Rim, I Hwang, CJ Lee, M Lee, CM Oh, JY Jeon, HY Gee, JH Kim, BW Lee, ES Kang, BS Cha, MS Lee, JW Yu, JW Cho, JS Kim, YH Lee SGLT2 inhibition modulates NLRP3 inflammasome activity via ketones and insulin in diabetes with cardiovascular disease Nat Commun, 2020-05-01;11(1):2127. 2020-05-01 [PMID: 32358544] (Human)
• L Janks, CVR Sharma, TM Egan A central role for P2X7 receptors in human microglia J Neuroinflammation, 2018-11-21;15(1):325. 2018-11-21 [PMID: 30463629] (Human)
• L Qian Decreased interleukin-11 levels in the semen of infertile males Cytokine, 2018-03-22;108(0):57-59. 2018-03-22 [PMID: 29573586] (Human)
• S Salimi, MD Shardell, SL Seliger, S Bandinelli, JM Guralnik, L Ferrucci Inflammation and Trajectory of Renal Function in Community-Dwelling Older Adults J Am Geriatr Soc, 2018-01-23;0(0):. 2018-01-23 [PMID: 29360163] (Human)
• YK Yong, HY Tan, SH Jen, EM Shankar, SK Natkunam, J Sathar, R Manikam, SD Sekaran Aberrant monocyte responses predict and characterize dengue virus infection in individuals with severe disease J Transl Med, 2017-05-31;15(1):121. 2017-05-31 [PMID: 28569153] (Human)