Human Total IL-18/IL-1F4 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Total IL-18/IL-1F4 Quantikine ELISA Kit Summary

• Background: The Quantikine® Human Total IL-18/IL-1F4 Immunoassay is a 4.0 hour solid-phase ELISA designed to measure human IL-18 in cell culture supernates, serum, plasma, saliva, and urine. It contains E. coli-expressed recombinant human IL-18 and has been shown to accurately quantitate the recombinant factor free and in complex with IL-18 BPa. Results obtained using natural human IL-18 showed linear ...curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-18.
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• Specificity: Natural and recombinant human Total IL-18.
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Total IL-18/IL-1F4 Quantikine ELISA Kit

• Iboctadekin
• IFN-gamma-inducing factor
• IGIF
• IGIFIL-1 gamma
• IL18
• IL-18
• IL-18MGC12320
• IL-1F4
• IL1F4iboctadekin
• IL-1g
• Interferon gamma-inducing factor
• interleukin 18 (interferon-gamma-inducing factor)
• Interleukin-1 gamma
• interleukin-18

Background

Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δT cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases. IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen independent production of IL-17 by gamma δ T cells and CD4+ T cells (11). IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature human IL-18 shares approximately 63% amino acid sequence identity with mouse and rat IL-18 (15). Alternative splicing in human ovarian cancer generates an isoform that is resistant to Caspase-1 activation (16). A cell surface form can be expressed on M-CSF induced macrophages and released in response to bacterial endotoxin (17).

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-18/IL-1F4 (DL180)(27)

Publications using DL180

• Koumangoye, RB;Ferdaus, MZ;Davis, X;Bohannon, JK;Delpire, E; Loss of NKCC1 activates the NLRP3 inflammasome in intestinal epithelia Cellular and molecular gastroenterology and hepatology 2025-11-17 [PMID: 41260571] (Mouse)
• He, Y;Liu, J;Zhou, N;Xie, LX;Jiang, YF;Chen, CL; Peroxiredoxin 1 inhibits tumorigenesis by activating the NLRP3/GSDMD pathway to induce pyroptosis of colorectal cancer cells World journal of gastroenterology 2025-09-28 [PMID: 41025079] (Human)
• Martinez Valenzuela, L;Vidal-Alabró, A;Rubio, B;Antón-Pàmpols, P;Gómez-Preciado, F;Fulladosa, X;Cruzado, JM;Torras, J;Lloberas, N;Draibe, J; Evaluating Single-Nucleotide Polymorphisms in Inflammasome Proteins and Serum Levels of IL-18 and IL-1? in Kidney Interstitial Damage in Anti-Neutrophilic Cytoplasmic Antibody-Associated Vasculitis International journal of molecular sciences 2024-06-12 [PMID: 38928186] (Human)
• Neubauer-Geryk, J;Wielicka, M;My?liwiec, M;Zorena, K;Bieniaszewski, L; The Impact of Metabolic Memory on Immune Profile in Young Patients with Uncomplicated Type 1 Diabetes International journal of molecular sciences 2024-03-10 [PMID: 38542165] (Human)
• Kosmider, O;Possémé, C;Templé, M;Corneau, A;Carbone, F;Duroyon, E;Breillat, P;Chirayath, TW;Oules, B;Sohier, P;Luka, M;Gobeaux, C;Lazaro, E;Outh, R;Le Guenno, G;Lifermann, F;Berleur, M;Le Mene, M;Friedrich, C;Lenormand, C;Weitten, T;Guillotin, V;Burroni, B;Boussier, J;Willems, L;Aractingi, S;Dionet, L;Tharaux, PL;Vergier, B;Raynaud, P;Ea, HK;Ménager, M;Duffy, D;Terrier, B; VEXAS syndrome is characterized by inflammasome activation and monocyte dysregulation Nature communications 2024-01-30 [PMID: 38291039] (Human)
• Exconde, PM;Hernandez-Chavez, C;Bourne, CM;Richards, RM;Bray, MB;Lopez, JL;Srivastava, T;Egan, MS;Zhang, J;Yoo, W;Shin, S;Discher, BM;Taabazuing, CY; The tetrapeptide sequence of IL-18 and IL-1? regulates their recruitment and activation by inflammatory caspases Cell reports 2023-12-15 [PMID: 38103201] (Human)
• Musia?, K;Stojanowski, J;Mi?kiewicz-Bujna, J;Ka?wak, K;Ussowicz, M; KIM-1, IL-18, and NGAL, in the Machine Learning Prediction of Kidney Injury among Children Undergoing Hematopoietic Stem Cell Transplantation-A Pilot Study International journal of molecular sciences 2023-10-31 [PMID: 37958774] (Human)
• Nabatanzi, R;Ssekamatte, P;Castelnuovo, B;Kambugu, A;Nakanjako, D; Increased Levels of Caspase-1 and IL-1? Among Adults With Persistent Immune Activation After 12 Years of Suppressive Antiretroviral Therapy in the Infectious Diseases Institute HIV Treatment Cohort Open forum infectious diseases 2023-11-01 [PMID: 37953818] (Human)
• SR Schnittman, DW Kitch, TH Swartz, TH Burdo, KV Fitch, S McCallum, JM Flynn, ES Fulda, MR Diggs, JT Stapleton, JL Casado, J Taron, JS Currier, MV Zanni, C Malvestutt, CJ Fichtenbau, JA Aberg, HJ Ribaudo, MT Lu, PS Douglas, SK Grinspoon Coronary Artery Plaque Composition and Severity Relate to the Inflammasome in People With Treated Human Immunodeficiency Virus Open forum infectious diseases, 2023-03-01;10(3):ofad106. 2023-03-01 [PMID: 36998633] (Human)
• LH Willems, LMC Jacobs, LA Groh, H Ten Cate, HMH Spronk, B Wilson-Sto, G Hannink, SMJ van Kuijk, C Ghossein-D, M Nagy, DHJ Thijssen, AS van Peters, MC Warlé Vascular Function, Systemic Inflammation, and Coagulation Activation 18 Months after COVID-19 Infection: An Observational Cohort Study Journal of Clinical Medicine, 2023-02-10;12(4):. 2023-02-10 [PMID: 36835948] (Human)
• J Milara, F Martínez-E, P Montero, I Roger, MA Bayarri, P Ribera, MN Oishi-Kona, JR Alba-Garcí, E Zapater, J Cortijo N-acetylcysteine Reduces Inflammasome Activation Induced by SARS-CoV-2 Proteins In Vitro International Journal of Molecular Sciences, 2022-11-22;23(23):. 2022-11-22 [PMID: 36498845] (Human)
• P Szczepanik, M Michalska-, M Kowal, D Krasowska Serum Levels of Selected IL-1 Family Cytokines in Patients with Morphea Journal of Clinical Medicine, 2022-10-28;11(21):. 2022-10-28 [PMID: 36362603] (Human)
• MC Chiang, CJB Nicol, SS Lo, SW Hung, CJ Wang, CH Lin Resveratrol Mitigates Oxygen and Glucose Deprivation-Induced Inflammation, NLRP3 Inflammasome, and Oxidative Stress in 3D Neuronal Culture International Journal of Molecular Sciences, 2022-10-02;23(19):. 2022-10-02 [PMID: 36232980] (Human)
• F Piancone, F La Rosa, I Marventano, A Hernis, R Miglioli, F Trecate, M Saresella, M Clerici Modulation of Neuroendocrine and Immunological Biomarkers Following Rehabilitation in Sarcopenic Patients Cells, 2022-08-10;11(16):. 2022-08-10 [PMID: 36010554] (Human)
• F La Rosa, CP Zoia, C Bazzini, A Bolognini, M Saresella, E Conti, C Ferrarese, F Piancone, I Marventano, D Galimberti, C Fenoglio, E Scarpini, M Clerici Modulation of MAPK- and PI3/AKT-Dependent Autophagy Signaling by Stavudine (D4T) in PBMC of Alzheimer's Disease Patients Cells, 2022-07-12;11(14):. 2022-07-12 [PMID: 35883623] (Human)
• DB Rosen, EA Murphy, RS Gejman, A Capili, RL Friedlande, S Rand, KA Cagino, SM Glynn, KC Matthews, JM Kubiak, J Yee, M Prabhu, LE Riley, YJ Yang Cytokine response over the course of COVID-19 infection in pregnant women Cytokine, 2022-04-25;154(0):155894. 2022-04-25 [PMID: 35490452] (Human)
• Tominaga T, Ikemura T, Yada K, Kanda K, Sugama K, Ma S, Choi W, Araya M, Huang J, Nakamura N, Suzuki K The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males. Antioxidants (Basel), 2021-05-28;10(6):. 2021-05-28 [PMID: 34071378] (Human)
• F La Rosa, S Agostini, M Saresella, AS Costa, F Piancone, R Miglioli, F Trecate, M Clerici Deregulation of IL-37 and its miRNAs modulators in sarcopenic patients after rehabilitation Journal of Translational Medicine, 2021-04-26;19(1):172. 2021-04-26 [PMID: 33902634] (Human)
• ML Matias, M Romao-Veig, VR Ribeiro, PR Nunes, VJ Gomes, AC Devides, VT Borges, GG Romagnoli, JC Peracoli, MT Peracoli Progesterone and vitamin D downregulate the activation of the NLRP1/NLRP3 inflammasomes and TLR4-MyD88-NF-&kappaB pathway in monocytes from pregnant women with preeclampsia Journal of reproductive immunology, 2021-02-05;144(0):103286. 2021-02-05 [PMID: 33578174] (Human)
• DM de Graaf, M Jaeger, ICL van den Mu, RT Horst, K Schraa, J Zwaag, M Kox, M Fujita, T Yamauchi, L Mercurio, S Madonna, JHW Rutten, J de Graaf, NP Riksen, FL van de Vee, MG Netea, LAB Joosten, CA Dinarello Reduced concentrations of the B cell cytokine Interleukin 38 are associated with cardiovascular disease risk in overweight subjects Eur J Immunol, 2020-11-19;0(0):. 2020-11-19 [PMID: 33125159] (Human)
• H Tsugawa, Y Kabe, A Kanai, Y Sugiura, S Hida, S Taniguchi, T Takahashi, H Matsui, Z Yasukawa, H Itou, K Takubo, H Suzuki, K Honda, H Handa, M Suematsu Short-chain fatty acids bind to apoptosis-associated speck-like protein to activate inflammasome complex to prevent Salmonella infection PLoS Biol, 2020-09-29;18(9):e3000813. 2020-09-29 [PMID: 32991574] (Mouse)
• FS Thaler, B Bangol, M Biljecki, J Havla, AM Schumacher, T Kümpfel Possible link of genetic variants to autoimmunity in GAD-antibody-associated neurological disorders J. Neurol. Sci., 2020-04-29;413(0):116860. 2020-04-29 [PMID: 32388243] (Human)
• SR Kim, SG Lee, SH Kim, JH Kim, E Choi, W Cho, JH Rim, I Hwang, CJ Lee, M Lee, CM Oh, JY Jeon, HY Gee, JH Kim, BW Lee, ES Kang, BS Cha, MS Lee, JW Yu, JW Cho, JS Kim, YH Lee SGLT2 inhibition modulates NLRP3 inflammasome activity via ketones and insulin in diabetes with cardiovascular disease Nat Commun, 2020-05-01;11(1):2127. 2020-05-01 [PMID: 32358544] (Human)
• L Janks, CVR Sharma, TM Egan A central role for P2X7 receptors in human microglia J Neuroinflammation, 2018-11-21;15(1):325. 2018-11-21 [PMID: 30463629] (Human)
• L Qian Decreased interleukin-11 levels in the semen of infertile males Cytokine, 2018-03-22;108(0):57-59. 2018-03-22 [PMID: 29573586] (Human)
• S Salimi, MD Shardell, SL Seliger, S Bandinelli, JM Guralnik, L Ferrucci Inflammation and Trajectory of Renal Function in Community-Dwelling Older Adults J Am Geriatr Soc, 2018-01-23;0(0):. 2018-01-23 [PMID: 29360163] (Human)
• YK Yong, HY Tan, SH Jen, EM Shankar, SK Natkunam, J Sathar, R Manikam, SD Sekaran Aberrant monocyte responses predict and characterize dengue virus infection in individuals with severe disease J Transl Med, 2017-05-31;15(1):121. 2017-05-31 [PMID: 28569153] (Human)

FAQs for IL-18/IL-1F4 (DL180). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.