Human Total IL-18 DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Total IL-18 DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL18

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Total IL-18 DuoSet ELISA, 5 Plate

• Iboctadekin
• IFN-gamma-inducing factor
• IGIF
• IGIFIL-1 gamma
• IL18
• IL-18
• IL-18MGC12320
• IL-1F4
• IL1F4iboctadekin
• IL-1g
• Interferon gamma-inducing factor
• interleukin 18 (interferon-gamma-inducing factor)
• Interleukin-1 gamma
• interleukin-18

Background

Interleukin 18 (IL-18), also known as interferon-gamma-inducing factor (IGIF) and IL-1γ, is a cytokine which shares biologic activities with IL-12 and structural similarities with the IL-1 family of proteins. IL-18 was originally cloned from liver cells and has since been shown to be expressed by monocyte/macrophages, osteoblasts and keratinocytes. Caspase-1 (IL-1 beta-converting enzyme) has been implicated in the physiological processing of pro-IL-18 to IL-18. Similarly to IL-12, human IL-18 has been shown to enhance NK cell activity in PBMC cultures. Human IL-18 has also been found to induce the production IFN-γ and GM-CSF while inhibiting the production of IL-10 by PBMC. On enriched human T cells, human IL-18 can enhance Th1 cytokine production and stimulate cell proliferation via an IL-2-dependent pathway. In the mouse system, IL-18 has been shown to be a costimulatory factor for the activation of Th1, but not Th2, cells. IL-18 was found to selectively enhance the FasL-mediated cytotoxicity of Th1, but not Th0 or Th2, cells. IL-18 has also been shown to induce activated B cells to produce IFN-γ that inhibits IgE production.

Publications for IL-18/IL-1F4 (DY318-05)(45)

Publications using DY318-05

• Schoenfeld, DA;Djureinovic, D;Su, DG;Zhang, L;Lu, BY;Kamga, L;Mann, JE;Huck, JD;Hurwitz, M;Braun, DA;Jilaveanu, L;Ring, AM;Kluger, HM; Decoy-resistant IL-18 reshapes the tumor microenvironment and enhances rejection by anti-CTLA-4 in renal cell carcinoma JCI insight 2024-11-19 [PMID: 39561007] (Human)
• Guha, A;Diaz-Pino, R;Fagbemi, A;Hughes, SM;Wynn, RF;Lopez-Castejon, G;Arkwright, PD; Very Early-Onset IBD-Associated IL-18opathy Treated with an Anti-IL-18 Antibody Journal of clinical medicine 2024-10-11 [PMID: 39458007] (Human)
• Cabrera, LE;Jokiranta, ST;Mäki, S;Miettinen, S;Kant, R;Kareinen, L;Sironen, T;Pietilä, JP;Kantele, A;Kekäläinen, E;Lindgren, H;Mattila, P;Kipar, A;Vapalahti, O;Strandin, T; The assembly of neutrophil inflammasomes during COVID-19 is mediated by type I interferons PLoS pathogens 2024-08-01 [PMID: 39172744] (Human)
• Szymanska, I;Bauernfried, S;Komar, T;Hornung, V; Vaccinia virus F1L blocks the ribotoxic stress response to subvert ZAK?-dependent NLRP1 inflammasome activation European journal of immunology 2024-07-31 [PMID: 39086059] (Human)
• Khongpraphan, S;Ekchariyawat, P;Sanongkiet, S;Luangjindarat, C;Sirisinha, S;Ponpuak, M;Midoeng, P;Pudla, M;Utaisincharoen, P; Differentiation in pyroptosis induction by Burkholderia pseudomallei and Burkholderia thailandensis in primary human monocytes, a possible cause of sepsis in acute melioidosis patients PLoS neglected tropical diseases 2024-07-01 [PMID: 39042701] (Human)
• Fischer?Riepe, L;Kailayangiri, S;Zimmermann, K;Pfeifer, R;Aigner, M;Altvater, B;Kretschmann, S;Völkl, S;Hartley, J;Dreger, C;Petry, K;Bosio, A;Döllen, A;Hartmann, W;Lode, H;Görlich, D;Mackensen, A;Jungblut, M;Schambach, A;Abken, H;Rössig, C; Preclinical Development of CAR T Cells with Antigen-Inducible IL18 Enforcement to Treat GD2-Positive Solid Cancers Clinical Cancer Research 2024-04-09 [PMID: 38593230] (Human)
• Andreou, D;Steen, NE;Mørch-Johnsen, L;Jørgensen, KN;Wortinger, LA;Barth, C;Szabo, A;O'Connell, KS;Lekva, T;Hjell, G;Johansen, IT;Ormerod, MBEG;Haukvik, UK;Aukrust, P;Djurovic, S;Yolken, RH;Andreassen, OA;Ueland, T;Agartz, I; Toxoplasma gondii infection associated with inflammasome activation and neuronal injury Scientific reports 2024-03-04 [PMID: 38438515] (Human)
• Huang, X;Kantonen, J;Nowlan, K;Nguyen, NA;Jokiranta, ST;Kuivanen, S;Heikkilä, N;Mahzabin, S;Kantele, A;Vapalahti, O;Myllykangas, L;Heinonen, S;Mäyränpää, MI;Strandin, T;Kekäläinen, E; Mucosal-Associated Invariant T Cells are not susceptible in vitro to SARS-CoV-2 infection but accumulate into the lungs of COVID-19 patients Virus research 2024-01-13 [PMID: 38211733] (Human)
• Oliviero, F;Baggio, C;Favero, M;Damasco, AC;Boscaro, C;Tietto, D;Albiero, M;Doria, A;Ramonda, R; Synovial Fluid from Patients with Osteoarthritis Shows Different Inflammatory Features Depending on the Presence of Calcium Pyrophosphate Crystals International journal of molecular sciences 2023-12-27 [PMID: 38203564] (Human)
• Parent, S;Vaka, R;St Amant, J;Kahn, S;Van Remortel, S;Bi, C;Courtman, D;Stewart, DJ;Davis, DR; Inactivation of the NLRP3 inflammasome mediates exosome-based prevention of atrial fibrillation Theranostics 2024-01-01 [PMID: 38169629] (Rat)
• Guan, X;Leng, W;Hu, Q;Xiu, M;Zhang, X; Association between cognitive function and IL-18 levels in schizophrenia: Dependent on IL18 - 607 A/C polymorphism Psychoneuroendocrinology 2023-09-12 [PMID: 37741261] (Human)
• Goulet, N;Tetzlaff, EJ;Morin, R;Mauger, JF;Amaratunga, R;Kenny, GP;Imbeault, P; No impact of a high-fat meal coupled with intermittent hypoxemia on acute kidney injury biomarkers in adults with and without obstructive sleep apnea Physiological reports 2023-09-01 [PMID: 37653582] (Human)
• Coufal, S;Kverka, M;Kreisinger, J;Thon, T;Rob, F;Kolar, M;Reiss, Z;Schierova, D;Kostovcikova, K;Roubalova, R;Bajer, L;Jackova, Z;Mihula, M;Drastich, P;Tresnak Hercogova, J;Novakova, M;Vasatko, M;Lukas, M;Tlaskalova-Hogenova, H;Jiraskova Zakostelska, Z; Serum TGF-?1 and CD14 Predicts Response to Anti-TNF-? Therapy in IBD Journal of immunology research 2023-06-20 [PMID: 37383609] (Human)
• Fiorillo, A;Gallego, JJ;Casanova-Ferrer, F;Giménez-Garzó, C;Urios, A;Ballester, MP;Durbán, L;Rios, MP;Megías, J;San Miguel, T;Kosenko, E;Escudero-García, D;Benlloch, S;Felipo, V;Montoliu, C; Mild Cognitive Impairment Is Associated with Enhanced Activation of Th17 Lymphocytes in Non-Alcoholic Fatty Liver Disease International journal of molecular sciences 2023-06-20 [PMID: 37373554] (Human)
• YS Lim, AG Lee, X Jiang, JM Scott, A Cofie, S Kumar, D Kennedy, DJ Granville, H Shin NK cell-derived extracellular granzyme B drives epithelial ulceration during HSV-2 genital infection Cell Reports, 2023-04-17;42(4):112410. 2023-04-17 [PMID: 37071533] (Human)
• SK Kim, JY Choe, KY Park CXCL12 and CXCR4 as Novel Biomarkers in Uric Acid-Induced Inflammation and Patients with Gouty Arthritis Biomedicines, 2023-02-21;11(3):. 2023-02-21 [PMID: 36979628] (Human)
• S Devi, M Indramohan, E Jäger, J Carriere, LH Chu, L de Almeida, DR Greaves, C Stehlik, A Dorfleutne CARD-only proteins regulate in�vivo inflammasome responses and ameliorate gout Cell Reports, 2023-03-16;42(3):112265. 2023-03-16 [PMID: 36930645] (Human)
• KCG Manfrere, MP Torrealba, FM Ferreira, ESA de Sousa, D Miyashiro, FME Teixeira, RWA Custódio, HI Nakaya, YAL Ramos, MN Sotto, A Woetmann, N Ødum, AJDS Duarte, JA Sanches, MN Sato Imbalanced IL-1B and IL-18 Expression in S�zary Syndrome International Journal of Molecular Sciences, 2023-02-28;24(5):. 2023-02-28 [PMID: 36902104] (Human)
• C Ge, J Tan, X Dai, Q Kuang, S Zhong, L Lai, C Yi, Y Sun, J Luo, C Zhang, L Zhu, B Wang, M Xu Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK Oncogene, 2022-10-08;13(1):5945. 2022-10-08 [PMID: 36209205] (Human)
• G Hjell, A Szabo, L Mørch-John, R Holst, N Tesli, C Bell, T Fischer-Vi, MCF Werner, SH Lunding, MBEG Ormerod, IT Johansen, I Dieset, S Djurovic, I Melle, T Ueland, OA Andreassen, NE Steen, UK Haukvik Interleukin-18 signaling system links to agitation in severe mental disorders Psychoneuroendocrinology, 2022-03-12;140(0):105721. 2022-03-12 [PMID: 35301151] (Human)
• A Lindblad, C Johansson, K Persson, I Demirel The role of caspase-1, caspase-4 and NLRP3 in regulating the host cell response evoked by uropathogenic Escherichia coli Scientific Reports, 2022-02-07;12(1):2005. 2022-02-07 [PMID: 35132157] (Human)
• J Villar, R Herrán-Mon, E González-H, M Prieto-Gon, A Ambrós, A Rodríguez-, A Muriel-Bom, R Solano, C Cuenca-Rub, A Vidal, C Flores, JM González-M, MI García-Lao, Genetics o Clinical and biological markers for predicting ARDS and outcome in septic patients Scientific Reports, 2021-11-22;11(1):22702. 2021-11-22 [PMID: 34811434] (Human)
• H Bonnekoh, C Vera, A Abad-Perez, S Radetzki, M Neuenschwa, E Specker, NA Mahnke, S Frischbutt, E Latz, M Nazaré, JV Kries, M Maurer, J Scheffel, K Krause Topical inflammasome inhibition with disulfiram prevents irritant contact dermatitis Clinical and translational allergy, 2021-07-22;11(5):e12045. 2021-07-22 [PMID: 34322217] (Human)
• E Jover, L Matilla, M Garaikoetx, A Fernández-, P Muntendam, F Jaisser, P Rossignol, N López-Andr Beneficial Effects of Mineralocorticoid Receptor Pathway Blockade against Endothelial Inflammation Induced by SARS-CoV-2 Spike Protein Biomedicines, 2021-06-03;9(6):. 2021-06-03 [PMID: 34204890] (Human)
• J Xing, X Zhou, M Fang, E Zhang, LJ Minze, Z Zhang DHX15 is required to control RNA virus-induced intestinal inflammation Cell Reports, 2021-06-22;35(12):109205. 2021-06-22 [PMID: 34161762] (Human)
• CY Wu, WL Fan, YM Chiu, HY Yang, WI Lee, JL Huang Lipopolysaccharide stimulation test on cultured PBMCs assists the discrimination of cryopyrin-associated periodic syndrome from systemic juvenile idiopathic arthritis Scientific Reports, 2021-06-07;11(1):11903. 2021-06-07 [PMID: 34099791] (Human)
• TM Yendo, MN Sato, ACCC Branco, AJ Pietrobon, FME Teixeira, YÁL Ramos, RW Alberca, CG Valêncio, VN Arruda, R Romiti, M Arnone, ALDS Hirayama, AJDS Duarte, V Aoki, RL Orfali Impact of Inflammatory Immune Dysfunction in Psoriasis Patients at Risk for COVID-19 Vaccines, 2021-05-10;9(5):. 2021-05-10 [PMID: 34068473] (Human)
• K Tejchman, A Nowacki, K Kotfis, E Skwirczyns, M Kotowski, L Zair, M Ostrowski, J Sienko The Role of Endothelins, IL-18, and NGAL in Kidney Hypothermic Machine Perfusion Biomedicines, 2021-04-13;9(4):. 2021-04-13 [PMID: 33924469] (Human)
• K Triantafil, CJK Ward, M Czubala, RG Ferris, E Koppe, C Haffner, V Piguet, VK Patel, H Amrine-Mad, L Modis, SL Masters, M Triantafil Differential recognition of HIV-stimulated IL-1&beta and IL-18 secretion through NLR and NAIP signalling in monocyte-derived macrophages PloS Pathogens, 2021-04-16;17(4):e1009417. 2021-04-16 [PMID: 33861800] (Human)
• G Tang, X Han, Z Lin, H Qian, B Chen, C Zhou, Y Chen, W Jiang Propionibacterium acnes Accelerates Intervertebral Disc Degeneration by Inducing Pyroptosis of Nucleus Pulposus Cells via the ROS-NLRP3 Pathway Oxidative Medicine and Cellular Longevity, 2021-02-01;2021(0):4657014. 2021-02-01 [PMID: 33603947] (Human)
• H Dai, O Penack, A Radujkovic, D Schult, J Majer-Laut, IW Blau, L Bullinger, S Jiang, C Müller-Tid, P Dreger, T Luft Early bilirubinemia after allogeneic stem cell transplantation-an endothelial complication Bone marrow transplantation, 2021-01-30;0(0):. 2021-01-30 [PMID: 33517355] (Human)
• A Radujkovic, T Boch, F Nolte, D Nowak, C Kunz, A Gieffers, C Müller-Tid, P Dreger, WK Hofmann, T Luft Clinical Response to the CD95-Ligand Inhibitor Asunercept Is Defined by a Pro-Inflammatory Serum Cytokine Profile Cancers, 2020-12-08;12(12):. 2020-12-08 [PMID: 33302451] (Human)
• A Radujkovic, L Kordelas, R Bogdanov, C Müller-Tid, DW Beelen, P Dreger, T Luft Interleukin-18 and Hematopoietic Recovery after Allogeneic Stem Cell Transplantation Cancers (Basel), 2020-09-28;12(10):. 2020-09-28 [PMID: 32998441] (Human)
• S Tumpara, B Martinez-D, G Gomez-Mari, B Liu, DS DeLuca, E Korenbaum, D Jonigk, F Jugert, FM Wurm, MJ Wurm, T Welte, S Janciauski The Delivery of &alpha1-Antitrypsin Therapy Through Transepidermal Route: Worthwhile to Explore Front Pharmacol, 2020-07-03;11(0):983. 2020-07-03 [PMID: 32719598] (Human)
• A Hedbrant, L Andersson, IL Bryngelsso, D Eklund, H Westberg, E Särndahl, A Persson Quartz Dust Exposure Affects NLRP3 Inflammasome Activation and Plasma Levels of IL-18 and IL-1Ra in Iron Foundry Workers Mediators Inflamm., 2020-01-07;2020(0):8490908. 2020-01-07 [PMID: 32256196] (Human)
• K Zimmermann, J Kuehle, AC Dragon, M Galla, C Kloth, LS Rudek, IE Sandalciog, B Neyazi, T Moritz, J Meyer, C Rossig, B Altvater, B Eiz-Vesper, MA Morgan, H Abken, A Schambach Design and Characterization of an "All-in-One" Lentiviral Vector System Combining Constitutive Anti-GD2 CAR Expression and Inducible Cytokines Cancers (Basel), 2020-02-06;12(2):. 2020-02-06 [PMID: 32041222] (Human)
• K Wedervang-, S Friis, V Lonning, RE Smelror, C Johannesse, EJ Reponen, SH Lyngstad, T Lekva, P Aukrust, T Ueland, OA Andreassen, I Agartz, AM Myhre Increased interleukin 18 activity in adolescents with early-onset psychosis is associated with cortisol and depressive symptoms Psychoneuroendocrinology, 2019-11-14;0(0):104513. 2019-11-14 [PMID: 31761332] (Human)
• A Merz, U Germing, G Kobbe, J Kaivers, A Jauch, A Radujkovic, M Hummel, A Benner, M Merz, P Dreger, T Luft EASIX for prediction of survival in lower-risk myelodysplastic syndromes Blood Cancer J, 2019-11-11;9(11):85. 2019-11-11 [PMID: 31712595] (Human)
• G Müller, C Lübow, G Weindl Lysosomotropic beta blockers induce oxidative stress and IL23A production in Langerhans cells Autophagy, 2019-11-07;0(0):1-16. 2019-11-07 [PMID: 31668121] (Human)
• VB Lê, J Dubois, C Couture, MH Cavanagh, O Uyar, A Pizzorno, M Rosa-Calat, MÈ Hamelin, G Boivin Human metapneumovirus activates NOD-like receptor protein 3 inflammasome via its small hydrophobic protein which plays a detrimental role during infection in mice PLoS Pathog., 2019-04-09;15(4):e1007689. 2019-04-09 [PMID: 30964929] (Human)
• E Kaasinen, O Kuismin, K Rajamäki, H Ristolaine, M Aavikko, J Kondelin, S Saarinen, DG Berta, R Katainen, EAM Hirvonen, A Karhu, A Taira, T Tanskanen, A Alkodsi, M Taipale, E Morgunova, K Franssila, R Lehtonen, M Mäkinen, K Aittomäki, A Palotie, MI Kurki, O Pietiläine, M Hilpert, E Saarentaus, J Niinimäki, J Junttila, K Kaikkonen, P Vahteristo, RC Skoda, MRJ Seppänen, KK Eklund, J Taipale, O Kilpivaara, LA Aaltonen Impact of constitutional TET2 haploinsufficiency on molecular and clinical phenotype in humans Nat Commun, 2019-03-19;10(1):1252. 2019-03-19 [PMID: 30890702] (Human)
• A Radujkovic, H Dai, L Kordelas, D Beelen, SP Rachakonda, C Müller-Tid, R Kumar, P Dreger, T Luft Asymmetric dimethylarginine serum levels are associated with early mortality after allogeneic stem cell transplantation Haematologica, 2018-12-04;0(0):. 2018-12-04 [PMID: 30514796] (Human)
• N Hassan, A Ali, C Withycombe, M Ahluwalia, RH Al-Nasseri, A Tonks, K Morris TET-2 up-regulation is associated with the anti-inflammatory action of Vicenin-2 Cytokine, 2018-03-19;108(0):37-42. 2018-03-19 [PMID: 29567563] (Human)
• J Venturini, RS Cavalcante, DV Moris, M de Assis G, AD Levorato, KH Dos Reis, MSP de Arruda, RP Mendes Altered distribution of peripheral blood dendritic cell subsets in patients with pulmonary paracoccidioidomycosis Acta Trop., 2017-06-09;0(0):. 2017-06-09 [PMID: 28606816] (Human)
• GR Kathamuthu, K Moideen, D Baskaran, VV Banurekha, D Nair, G Sekar, R Sridhar, B Vidyajayan, G Gajendrara, DK Parandhama, A Srinivasan, S Babu TB lymphadenitis is associated with enhanced baseline and antigen - specific induction of Type 1 and Type 17 cytokines and reduced IL-1? and IL-18 at the site of infection Clin. Vaccine Immunol, 2017-05-05;0(0):. 2017-05-05 [PMID: 28249842] (Human)

Bioinformatics

• Gene Symbol: IL18