Human Serum Albumin Antibody [FITC]

Dot Blot: Human Serum Albumin Antibody [FITC] [NBP2-44227] - Dot Blot results of Rabbit Human Serum Albumin antibody [FITC].Dots are Human Serum Albumin: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none.Secondary Antibody: Rabbit Anti-Human Serum Albumin Antibody FITC at 1ug/mL in 1% Fish Gel 1hr RT.Imaged with BioRad ChemiDoc, Fluorescein filter.

Dot Blot results of Rabbit Human Serum Albumin Antibody [FITC]. Dots are Human Serum Albumin: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Rabbit Anti-Human Serum Albumin Antibody FITC at 1ug/mL in 1% Fish Gel 1hr RT. Imaged with BioRad ChemiDoc, Fluorescein filter.

Product Discontinued

Human Serum Albumin Antibody [FITC] Summary

• Description: Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  This product was prepared from monospecific antiserum by immunoaffinity chromatography using purified human serum albumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein and anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.
• Immunogen: Albumin (Human Serum) (Uniprot: P02768)
• Specificity: Cross reactivity against Albumin from other tissues and species may occur but has not been specifically determined.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • Fluorophore-linked immunosorbent assay 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20-1:50
  • Western Blot
• Application Notes: This product has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Reactivity Notes

Cross reactivity against Human Serum Albumin from other tissues and species may occur but has not been specifically determined

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Polyethylene Glycol (PEG-8000)
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 1.0 ml deionized water (or equivalent)

Alternate Names for Human Serum Albumin Antibody [FITC]

• albumin
• cell growth inhibiting protein 42
• DKFZp779N1935
• growth-inhibiting protein 20
• PRO0883
• PRO0903
• PRO1341
• serum albumin

Background

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALB
• Uniprot:
  • P02768(Human)