Human/Rat Total HIF-2 alpha/EPAS1 DuoSet IC ELISA, 2 Plate

Lysate prepared from HepG2 human hepatocellular carcinoma cells treated with 150 μM of CoCl2 for 18 hours were incubated in wells coated with Total HIF-2 alpha Capture Antibody. Unbound material was removed by washing and bound material was solubilized in SDS gel sample buffer. The same lysate and captured proteins were electrophoresed and transferred to a PVDF membrane. The captured protein was immunoblotted with Total HIF-2 alpha Detection Antibody and the lysate was immunoblotted with anti-HIF-2 alpha antibody. Only one band corresponding to HIF-2 alpha was detected in the captured material.Cross-reactivity experiments were performed with this DuoSet IC ELISA to further determine specificity. Recombinant mouse HIF-2 alpha was assayed at 80 ng/mL and read 16.4 ng/mL (20.5% cross-reactivity). Recombinant human (rh) HIF-1 alpha and rhARNT were assayed at 80 ng/mL and did not cross-react or interfere in the assay.

Lysate prepared from 786-O human renal adenocarcinoma cells, Caki-2 human kidney clear carcinoma cells, and MCF-7 human breast cancer cells untreated or treated with 150 μM of CoCl2 for 18 hours were analyzed with both this DuoSet IC ELISA and the Human/Mouse Total HIF-1 alpha DuoSet IC ELISA (R&D Systems, Catalog # DYC1935). The 786-O cells are positive for HIF-2 alpha only while Caki-2 and MCF-7 treated with CoCl2 are positive for HIF-1 alpha only. Only the HIF-2 alpha positive 786-O cells were detected with the Human/Rat Total HIF-2 alpha /EPAS1 DuoSet IC ELISA, indicating that this DuoSet IC ELISA is specific for HIF-2 alpha .

Lysates prepared from 786-O human renal adenocarcinoma cell and HepG2 human hepatocellular carcinoma cells untreated or treated with 150 μM of CoCl2 for 18 hours were quantified with this DuoSet IC ELISA. The same lysates were also immunoblotted (inset) with anti-HIF-2 alpha antibody. The DuoSet IC ELISA results correlated well with the relative amounts of HIF-2 alpha detected by Western blot.

Lysates prepared from PC-12 rat adrenal pheochromocytoma cells untreated or treated with 150 μM of CoCl2 for 18 hours were quantified with this DuoSet IC ELISA. The same lysates were also immunoblotted (inset) with anti-HIF-2 alpha antibody (R&D Systems, Catalog # AF2886). The DuoSet IC ELISA results correlate well with the relative amounts of HIF-2 alpha detected by Western blot.

Product Discontinued

Human/Rat Total HIF-2 alpha/EPAS1 DuoSet IC ELISA, 2 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: EPAS1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human/Rat Total HIF-2 alpha/EPAS1 DuoSet IC ELISA, 2 Plate

• Basic-helix-loop-helix-PAS protein MOP2
• BHLHE73
• Class E basic helix-loop-helix protein 73
• ECYT4
• endothelial PAS domain protein 1
• endothelial PAS domain-containing protein 1
• EPAS1
• EPAS-1
• HIF 2A
• HIF-1-alpha-like factor
• HIF-1alpha-like factor
• HIF2 alpha
• HIF-2 alpha
• hif2a angiogenesis
• HIF2A
• HIF-2-alpha
• HIF2-alpha
• HLF
• hypoxia-inducible factor 2 alpha
• Hypoxia-inducible factor 2-alpha
• Member of PAS protein 2
• MOP2
• PAS domain-containing protein 2
• PASD2

Background

The hypoxia-inducible transcription factor 2 alpha is stabilized in hypoxic tissue and, similarly to HIF-1 alpha, complexes with aryl hydrocarbon receptor nuclear translocator (ARNT). Both the HIF-1 and HIF-2 complexes bind hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. HIF-1 and HIF-2 do not appear to be completely redundant, although specific functions are only beginning to be elucidated. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, indicating the potentially broad impact of gene regulation by both HIF-1 alpha and HIF-2 alpha.

Publications for HIF-2 alpha/EPAS1 (DYC2997-2)(2)

Publications using DYC2997-2

• Wei Shan, Yunfei Wang, Zhen Zhang, Jie Xing, Jienan Xu, Wen Xiao et al. Qingre Baidu mixture‑induced effect of AI‑2 on Staphylococcus�aureus and Pseudomonas�aeruginosa biofilms in chronic and refractory wounds Experimental and Therapeutic Medicine 2019-03-13 [PMID: 30988710] (Bacteria)
• Zhang , Yanqiong, Bai , Ming, Zhang , Bo, Liu , Chunfang, Guo , Qiuyan, Sun , Yanqun, Wang , Danhua, Wang , Chao, Jiang , Yini, Lin , Na, Li , Shao Uncovering pharmacological mechanisms of Wu-tou decoction acting on rheumatoid arthritis through systems approaches: drug-target prediction, network analysis and experimental validation. Sci Rep, 2015-03-30;5(0):9463. 2015-03-30 [PMID: 25820382] (Rat)

FAQs for HIF-2 alpha/EPAS1 (DYC2997-2). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: EPAS1