Human Pro-MMP-1 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Pro-MMP-1 Quantikine ELISA Kit Summary

• Background: The Quantikine Human Pro-MMP-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure Pro-MMP-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Pro-MMP-1 and antibodies raised against the recombinant factor. The antibodies will not recognize recombinant human active MMP-1 or TIMP bound MMP-1. Natural human Pro-MMP-1 showed dose-response cur...ves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative mass values of natural human Pro-MMP-1.
  Show More
• Specificity: Natural and recombinant human Pro-MMP-1
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: MMP1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 degreesC. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Pro-MMP-1 Quantikine ELISA Kit

• CLGmatrix metalloprotease 1
• CLGN
• EC 3.4.24
• EC 3.4.24.7
• Fibroblast collagenase
• interstitial collagenase
• matrix metallopeptidase 1 (interstitial collagenase)
• matrix metalloproteinase 1 (interstitial collagenase)
• Matrix metalloproteinase-1
• MMP1
• MMP-1

Background

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for MMP-1 (DMP100)(44)

Publications using DMP100

• Sengprasert, P;Waitayangkoon, P;Kamenkit, O;Sawatpanich, A;Chaichana, T;Wongphoom, J;Ngarmukos, S;Taweevisit, M;Lotinun, S;Tumwasorn, S;Tanavalee, A;Reantragoon, R; Catabolic mediators from TLR2-mediated proteoglycan aggrecan peptide-stimulated chondrocytes are reduced by Lactobacillus-conditioned media Scientific reports 2024-08-05 [PMID: 39103466] (Human)
• Kleine, A;Kühle, M;Ly, TD;Schmidt, V;Faust-Hinse, I;Knabbe, C;Fischer, B; Xylosyltransferase-Deficiency in Human Dermal Fibroblasts Induces Compensatory Myofibroblast Differentiation and Long-Term ECM Reduction Biomedicines 2024-03-04 [PMID: 38540185] (Human)
• Shen, ZQ;Chang, CY;Yeh, CH;Lu, CK;Hung, HC;Wang, TW;Wu, KS;Tung, CY;Tsai, TF; Hesperetin activates CISD2 to attenuate senescence in human keratinocytes from an older person and rejuvenates naturally aged skin in mice Journal of biomedical science 2024-01-23 [PMID: 38263133] (Human)
• I Tsomidis, G Notas, C Xidakis, A Voumvourak, DN Samonakis, M Koulentaki, E Kouroumali Enzymes of Fibrosis in Chronic Liver Disease Biomedicines, 2022-12-08;10(12):. 2022-12-08 [PMID: 36551935] (Human)
• J Shen, Z Wang, W Zhao, Y Fu, B Li, J Cheng, Y Deng, S Li, H Li TGF-beta1 induces type I collagen deposition in granulosa cells via the AKT/GSK-3beta signaling pathway-mediated MMP1 down-regulation Reproductive biology, 2022-10-26;22(4):100705. 2022-10-26 [PMID: 36308873] (Human)
• L Zhu, Y Wang, F Qiao microRNA-223 and microRNA-126 are clinical indicators for predicting the plaque stability in carotid atherosclerosis patients Journal of human hypertension, 2022-10-03;0(0):. 2022-10-03 [PMID: 36192429] (Human)
• TW Kim, AG Kim, KH Lee, MH Hwang, H Choi Microfluidic Electroceuticals Platform for Therapeutic Strategies of Intervertebral Disc Degeneration: Effects of Electrical Stimulation on Human Nucleus Pulposus Cells under Inflammatory Conditions International Journal of Molecular Sciences, 2022-09-04;23(17):. 2022-09-04 [PMID: 36077518] (Human)
• JH Shin, S Park, H Cho, JH Kim, H Choi Adipokine human Resistin promotes obesity-associated inflammatory intervertebral disc degeneration via pro-inflammatory cytokine cascade activation Scientific Reports, 2022-05-27;12(1):8936. 2022-05-27 [PMID: 35624126] (Human)
• J Jarecki, T Ma?ecka-Ma, E Kosior-Jar, W Widuchowsk, P Krasowski, M Gutbier, M Dobrzy?ski, T Blicharski Concentration of Selected Metalloproteinases and Osteocalcin in the Serum and Synovial Fluid of Obese Women with Advanced Knee Osteoarthritis International Journal of Environmental Research and Public Health, 2022-03-16;19(6):. 2022-03-16 [PMID: 35329213] (Human)
• J Woo, S Shin, E Cho, D Ryu, D Garandeau, H Chajra, M Fréchet, D Park, E Jung Senotherapeutic-like effect of Silybum marianum flower extract revealed on human skin cells PLoS ONE, 2021-12-16;16(12):e0260545. 2021-12-16 [PMID: 34914725] (Human)
• A Skurska, V Dymicka-Pi, R Milewski, M Pietruska Dynamics of Matrix Metalloproteinase-1 and -8 Secretion in Gingival Crevicular Fluid after Gingival Recession Therapy via MCAT with Either Subepithelial Connective Tissue Graft or Collagen Matrix Biomolecules, 2021-05-14;11(5):. 2021-05-14 [PMID: 34068848] (Human)
• J Jarecki, T Ma?ecka-Ma, I Polkowska, B Potoczniak, E Kosior-Jar, I Szerb, E Tomaszewsk, M Gutbier, M Dobrzy?ski, T Blicharski Level of Adiponectin, Leptin and Selected Matrix Metalloproteinases in Female Overweight Patients with Primary Gonarthrosis Journal of Clinical Medicine, 2021-03-18;10(6):. 2021-03-18 [PMID: 33803785] (Human)
• M Yeom, H Lee, S Shin, D Park, E Jung PER, a Circadian Clock Component, Mediates the Suppression of MMP-1 Expression in HaCaT Keratinocytes by cAMP Molecules, 2018-03-23;23(4):. 2018-03-23 [PMID: 29570674] (Human)
• V Schmitt, M Hahn, V Kästele, O Wagner, M Wiendl, A Derer, A Taddeo, S Hahne, A Radbruch, HM Jäck, W Schuh, D Mielenz, S Gay, G Schett, AJ Hueber, S Frey Interleukin-36 receptor mediates the crosstalk between plasma cells and synovial fibroblasts Eur. J. Immunol., 2017-09-22;0(0):. 2017-09-22 [PMID: 28857172] (Human)
• Z Liu, C Li, X Meng, Y Bai, J Qi, J Wang, Q Zhou, W Zhang, X Zhang Hypoxia-inducible factor-l? mediates aggrecan and collagen ? expression via NOTCH1 signaling in nucleus pulposus cells during intervertebral disc degeneration Biochem. Biophys. Res. Commun., 2017-05-17;0(0):. 2017-05-17 [PMID: 28526405] (Human)
• Galectin-3 Induces a Pro-degradative/inflammatory Gene Signature in Human Chondrocytes, Teaming Up with Galectin-1 in Osteoarthritis Pathogenesis Sci Rep, 2016-12-16;6(0):39112. 2016-12-16 [PMID: 27982117] (Human)
• Kmiecik A, Pula B, Suchanski J, Olbromski M, Gomulkiewicz A, Owczarek T, Kruczak A, Ambicka A, Rys J, Ugorski M, Podhorska-Okolow M, Dziegiel P Metallothionein-3 Increases Triple-Negative Breast Cancer Cell Invasiveness via Induction of Metalloproteinase Expression. PLoS ONE, 2015-05-01;10(5):e0124865. 2015-05-01 [PMID: 25933064] (Human)
• Herrera I, Cisneros J, Maldonado M, Ramirez R, Ortiz-Quintero B, Anso E, Chandel N, Selman M, Pardo A Matrix metalloproteinase (MMP)-1 induces lung alveolar epithelial cell migration and proliferation, protects from apoptosis, and represses mitochondrial oxygen consumption. J Biol Chem, 2013-07-31;288(36):25964-75. 2013-07-31 [PMID: 23902766] (Human)
• Li R, Ackerman WE, Mihai C Myoferlin depletion in breast cancer cells promotes mesenchymal to epithelial shape change and stalls invasion. PLoS ONE, 2012-06-27;7(6):e39766. 2012-06-27 [PMID: 22761893] (Human)
• Symeonidis C, Papakonstantinou E, Androudi S, Tsaousis KT, Tsinopoulos I, Brazitikos P, Karakiulakis G, Diza E, Dimitrakos SA Interleukin-6 and matrix metalloproteinase expression in the subretinal fluid during proliferative vitreoretinopathy: correlation with extent, duration of RRD and PVR grade. Cytokine, 2012-05-10;59(1):184-90. 2012-05-10 [PMID: 22579111] (Human)
• Wang JH, Shih KS, Liou JP, Wu YW, Chang AS, Wang KL, Tsai CL, Yang CR Anti-arthritic effects of magnolol in human interleukin 1β-stimulated fibroblast-like synoviocytes and in a rat arthritis model. PLoS ONE, 2012-02-16;7(2):e31368. 2012-02-16 [PMID: 22359588] (Human)
• Hashizume M, Mihara M High molecular weight hyaluronic acid inhibits IL-6-induced MMP production from human chondrocytes by up-regulating the ERK inhibitor, MKP-1. Biochem. Biophys. Res. Commun., 2010-11-06;403(2):184-9. 2010-11-06 [PMID: 21059338] (Human)
• Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation. Eur. J. Haematol., 2009-11-17;84(3):239-51. 2009-11-17 [PMID: 19922462] (Human)
• Karapanagiotidis GT, Antonitsis P, Charokopos N, Foroulis CN, Anastasiadis K, Rouska E, Argiriadou H, Rammos K, Papakonstantinou C Serum levels of matrix metalloproteinases -1,-2,-3 and -9 in thoracic aortic diseases and acute myocardial ischemia. J Cardiothorac Surg, 2009-11-03;4(0):59. 2009-11-03 [PMID: 19886986] (Human)
• Hayer S, Pundt N, Peters MA, Wunrau C, Kuhnel I, Neugebauer K, Strietholt S, Zwerina J, Korb A, Penninger J, Joosten LA, Gay S, Ruckle T, Schett G, Pap T PI3Kgamma regulates cartilage damage in chronic inflammatory arthritis. FASEB J., 2009-09-04;23(12):4288-98. 2009-09-04 [PMID: 19734303] (Human)
• Wunrau C, Schnaeker EM, Freyth K, Pundt N, Wendholt D, Neugebauer K, Hansen U, Pap T, Dankbar B Establishment of a matrix-associated transepithelial resistance invasion assay to precisely measure the invasive potential of synovial fibroblasts. Arthritis Rheum., 2009-09-01;60(9):2606-11. 2009-09-01 [PMID: 19714628] (Human)
• Roderfeld M, Rath T, Schulz R, Seeger W, Tschuschner A, Graf J, Roeb E Serum matrix metalloproteinases in adult CF patients: Relation to pulmonary exacerbation. J. Cyst. Fibros., 2009-07-14;8(5):338-47. 2009-07-14 [PMID: 19604728] (Human)
• Amiable N, Tat SK, Lajeunesse D, Duval N, Pelletier JP, Martel-Pelletier J, Boileau C Proteinase-activated receptor (PAR)-2 activation impacts bone resorptive properties of human osteoarthritic subchondral bone osteoblasts. Bone, 2009-03-02;44(6):1143-50. 2009-03-02 [PMID: 19264156] (Human)
• Zayed N, Afif H, Chabane N, Mfuna-Endam L, Benderdour M, Martel-Pelletier J, Pelletier JP, Motiani RK, Trebak M, Duval N, Fahmi H Inhibition of interleukin-1beta-induced matrix metalloproteinases 1 and 13 production in human osteoarthritic chondrocytes by prostaglandin D2. Arthritis Rheum., 2008-11-01;58(11):3530-40. 2008-11-01 [PMID: 18975308] (Human)
• Attur M, Al-Mussawir HE, Patel J, Kitay A, Dave M, Palmer G, Pillinger MH, Abramson SB Prostaglandin E2 exerts catabolic effects in osteoarthritis cartilage: evidence for signaling via the EP4 receptor. J. Immunol., 2008-10-01;181(7):5082-8. 2008-10-01 [PMID: 18802112] (Human)
• Agarwal A, Covic L, Sevigny LM, Kaneider NC, Lazarides K, Azabdaftari G, Sharifi S, Kuliopulos A Targeting a metalloprotease-PAR1 signaling system with cell-penetrating pepducins inhibits angiogenesis, ascites, and progression of ovarian cancer. Mol. Cancer Ther., 2008-09-01;7(9):2746-57. 2008-09-01 [PMID: 18790755] (Human)
• Rosas IO, Richards TJ, Konishi K, Zhang Y, Gibson K, Lokshin AE, Lindell KO, Cisneros J, Macdonald SD, Pardo A, Sciurba F, Dauber J, Selman M, Gochuico BR, Kaminski N MMP1 and MMP7 as potential peripheral blood biomarkers in idiopathic pulmonary fibrosis. PLoS Med., 2008-04-29;5(4):e93. 2008-04-29 [PMID: 18447576] (Human)
• Naveau A, Reinald N, Fournier B Gingival fibroblasts inhibit MMP-1 and MMP-3 activities in an ex-vivo artery model. Connect. Tissue Res., 2007-01-01;48(6):300-8. 2007-01-01 [PMID: 18075816] (Human)
• Leone G, Torricelli P, Chiumiento A, Facchini A, Barbucci R Amidic alginate hydrogel for nucleus pulposus replacement. J Biomed Mater Res A, 2008-02-01;84(2):391-401. 2008-02-01 [PMID: 17618483] (Human)
• Zhao Z, McCloud B, Fleming R, Klempner MS Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro. Biochem. Biophys. Res. Commun., 2007-05-02;358(2):528-33. 2007-05-02 [PMID: 17485071] (Human)
• Nah SS, Choi IY, Yoo B, Kim YG, Moon HB, Lee CK Advanced glycation end products increases matrix metalloproteinase-1, -3, and -13, and TNF-alpha in human osteoarthritic chondrocytes. FEBS Lett., 2007-04-09;581(9):1928-32. 2007-04-09 [PMID: 17434489] (Human)
• Sherman JA, Hall A, Malenka DJ, De Muinck ED, Simons M Humoral and cellular factors responsible for coronary collateral formation. Am. J. Cardiol., 2006-08-31;98(9):1194-7. 2006-08-31 [PMID: 17056326] (Human)
• Fearon U, Mullan R, Markham T, Connolly M, Sullivan S, Poole AR, FitzGerald O, Bresnihan B, Veale DJ Oncostatin M induces angiogenesis and cartilage degradation in rheumatoid arthritis synovial tissue and human cartilage cocultures. Arthritis Rheum., 2006-10-01;54(10):3152-62. 2006-10-01 [PMID: 17009243] (Human)
• Wolk K, Witte E, Wallace E, Docke WD, Kunz S, Asadullah K, Volk HD, Sterry W, Sabat R IL-22 regulates the expression of genes responsible for antimicrobial defense, cellular differentiation, and mobility in keratinocytes: a potential role in psoriasis. Eur. J. Immunol., 2006-05-01;36(5):1309-23. 2006-05-01 [PMID: 16619290] (Human)
• Ehling A, Schaffler A, Herfarth H, Tarner IH, Anders S, Distler O, Paul G, Distler J, Gay S, Scholmerich J, Neumann E, Muller-Ladner U The potential of adiponectin in driving arthritis. J. Immunol., 2006-04-01;176(7):4468-78. 2006-04-01 [PMID: 16547285] (Human)
• Domeij H, Modeer T, Quezada HC, Yucel-Lindberg T Cell expression of MMP-1 and TIMP-1 in co-cultures of human gingival fibroblasts and monocytes: the involvement of ICAM-1. Biochem. Biophys. Res. Commun., 2005-11-02;338(4):1825-33. 2005-11-02 [PMID: 16288711] (Human)
• Li DQ, Chen Z, Song XJ, Luo L, Pflugfelder SC Stimulation of matrix metalloproteinases by hyperosmolarity via a JNK pathway in human corneal epithelial cells. Invest. Ophthalmol. Vis. Sci., 2004-12-01;45(12):4302-11. 2004-12-01 [PMID: 15557436] (Human)
• Ulrich D, Hrynyschyn K, Pallua N Matrix metalloproteinases and tissue inhibitors of metalloproteinases in sera and tissue of patients with Dupuytren's disease. Plast. Reconstr. Surg., 2003-10-01;112(5):1279-86. 2003-10-01 [PMID: 14504511] (Human)
• D'Agostino P Matrix metalloproteinases production in malignant pleural effusions after talc pleurodesis. Clin. Exp. Immunol., 2003-10-01;134(1):138-42. 2003-10-01 [PMID: 12974766] (Human)

FAQs for MMP-1 (DMP100). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: MMP1