Human Pref-1/DLK1/FA1 DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Pref-1/DLK1/FA1 DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: DLK1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Pref-1/DLK1/FA1 DuoSet ELISA, 5 Plate

• delta-like 1 homolog (Drosophila)
• DLK
• DLK1
• DLK-1
• FA1
• FA1fetal antigen 1
• pG2
• pG2delta-like homolog (Drosophila)
• preadipocyte factor 1
• Pref1
• Pref-1
• protein delta homolog 1
• secredeltin
• ZOG

Background

Pref-1 (preadipocyte factor 1), also known as deltalike 1 (DLK1) and fetal antigen 1 (FA1), is a member of the Notch/Delta/Serrate protein family. It is a transmembrane protein that can be proteolyically cleaved to generate soluble forms. Pref-1 is believed to be a co-ligand for Notch signaling, enhancing or inhibiting Notch activation following binding of a ligand. It is expressed in numerous endocrine cells, and is highly expressed in preadipocytes, but not in mature adipocytes. In adipose tissue, Pref-1 inhibits adipogenesis and is often used as a preadipocyte marker.

Publications for Pref-1/DLK1/FA1 (DY1144-05)(1)

Publication using DY1144-05

• Wurst U, Ebert T, Kralisch S, Stumvoll M, Fasshauer M Serum levels of the adipokine Pref-1 in gestational diabetes mellitus. Cytokine, 2014-11-17;71(2):161-4. 2014-11-17 [PMID: 25461394] (Human)

FAQs for Pref-1/DLK1/FA1 (DY1144-05). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: DLK1