Human Phospho-MSPR/Ron DuoSet IC ELISA, 2 Plate

MDA-MB-453 human breast cancer cells were treated with 400 ng/mL recombinant human MSP (Catalog # 352-MS) for five minutes to induce tyrosine phosphorylation of MSP R. Serial dilutions of lysates were analyzed by (A) IP-Western Blot and (B) this DuoSet® IC ELISA. IPs were performed using an anti-MSP R monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with a biotinylated anti-phosphotyrosine monoclonal antibody (Catalog # BAM1676) to detect phospho-MSP R. Bands were visualized with Streptavidin-HRP (Catalog # DY998) followed by chemiluminescent detection. Human phospho-MSP R can be detected in this DuoSet® IC ELISA by using approximately 6-12 times less lysate than is needed for a conventional IP-Western Blot.

MDA-MB-453 human breast cancer cells were untreated or treated with 400 ng/mL recombinant human MSP for five minutes. ELISA and IP-Western Blot (inset) analyses were performed using 100 μg and 400 μg of lysate, respectively. IP-Western Blots for phospho-MSP R (p-MSP R) were performed as described in Figure 1. Blots were stripped and total MSP R was detected using a Biotinylated Anti-MSP R Polyclonal Antibody (Catalog # BAF691).

MDA-MB-453 human breast cancer cells were treated with 400 ng/mL recombinant human (rh) MSP for five minutes. The indicated amounts of recombinant extracellular domains of human MSP R, human HGF R/Fc Chimera (Catalog # 358-MT), human IGF-I sR (Catalog # 391-GR), or human EGF R (Catalog # 1095-ER) were added to 100 μg lysate and analyzed using this DuoSet® IC ELISA. Competition was observed only with recombinant human MSP R.

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Phospho-MSPR/Ron DuoSet IC ELISA, 2 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: MST1R

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Phospho-MSPR/Ron DuoSet IC ELISA, 2 Plate

• CD136 antigen
• CD136
• CDw136c-met-related tyrosine kinase
• EC 2.7.10
• EC 2.7.10.1
• macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
• MSP R
• MSP receptor
• MSPR
• MST1R
• p185-Ron
• Protein-tyrosine kinase 8
• PTK8 protein tyrosine kinase 8
• PTK8
• Ron
• RONmacrophage-stimulating protein receptor
• soluble RON variant 1
• soluble RON variant 2
• soluble RON variant 3
• soluble RON variant 4

Background

Macrophage stimulating protein receptor (MSPR), encoded by the human RON and the mouse Stk genes, is one of a small family of receptor tyrosine kinases (RTKs) that also includes human Met (the receptor for hepatocyte growth factor) and chicken Sea. This family of receptors is synthesized as a single-chain precursor that is cleaved into a mature disulfide-linked heterodimer composed of an extracellular a chain and a membrane spanning beta chain with intrinsic tyrosine kinase activity.

Publications for MSPR/Ron (DYC1947-2)(1)

Publication using DYC1947-2

• SK Masenga, F Elijovich, BM Hamooya, S Nzala, G Kwenda, DC Heimburger, W Mutale, SM Munsaka, S Zhao, JR Koethe, A Kirabo Elevated Eosinophils as a Feature of Inflammation Associated With Hypertension in Virally Suppressed People Living With HIV J Am Heart Assoc, 2020-02-17;9(4):e011450. 2020-02-17 [PMID: 32064996] (Human)

Bioinformatics

• Gene Symbol: MST1R