Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA, 2 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA, 2 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IGF1R

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 degreesC. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA, 2 Plate

• CD221 antigen
• CD221
• EC 2.7.10
• EC 2.7.10.1
• IGF1R
• IGF-1R
• IGF-I R
• IGF-I receptor
• IGFIR
• IGF-IR
• IGFR
• insulin-like growth factor 1 receptor
• Insulin-like growth factor I receptor
• JTK13
• MGC142170
• MGC142172
• MGC18216
• soluble IGF1R variant 1
• soluble IGF1R variant 2

Background

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. IGF-II R is a type I transmembrane glycoprotein that contains a 2,264 amino acid (aa) extracellular region, a 23 aa transmembrane segment segment and a 124 aa cytoplasmic tail. IGF-II R regulates many diverse biological functions that range from intracellular trafficking to the internalization of extracellular factors and modulation of cellular responses. It delivers newly synthesized M6P-tagged lysosomal enzymes from the trans-golgi network to endosomes, and facilitates the clearance of extracellular lysosomal and matrix degrading enzymes by internalization into clathrin-coated vesicles and delivery into endosomes. With respect to IGF-II biology, It would appear that IGF-II R is principally a regulator of local IGF-II levels, targeting IGF-II for destruction in lysosomes. The heterotetrameric receptors for insulin (INS R) and IGF-I (IGF-I R) are receptor tyrosine kinases that consist of two ligandbinding alpha subunits and two beta subunits. Ligand binding induces autophosphorylation on multiple tyrosine residues of beta subunits. Phosphorylation of Tyr1162 and 1163 on INS R and Tyr1135 and 1136 on IGF-I R stimulates intrinsic kinase activity.

Publications for IGF-I R/IGF1R (DYC1770-2)(6)

Publications using DYC1770-2

• Hjortebjerg, R;Høgdall, C;Hansen, KH;Høgdall, E;Frystyk, J; The IGF-PAPP-A-Stanniocalcin Axis in Serum and Ascites Associates with Prognosis in Patients with Ovarian Cancer International journal of molecular sciences 2024-02-07 [PMID: 38396692] (Human)
• G Vaniotis, S Moffett, T Sulea, N Wang, SM Elahi, E Lessard, J Baardsnes, S Perrino, Y Durocher, J Frystyk, B Massie, P Brodt Enhanced anti-metastatic bioactivity of an IGF-TRAP re-engineered to improve physicochemical properties Sci Rep, 2018-11-26;8(1):17361. 2018-11-26 [PMID: 30478273] (Human)
• Brouwer-Visser , Jurriaan, Lee , Jiyeon, McCullagh , KellyAnn, Cossio , Maria J, Wang , Yanhua, Huang , Gloria S Insulin-like growth factor 2 silencing restores taxol sensitivity in drug resistant ovarian cancer. PLoS ONE, 2014-06-16;9(6):e100165. 2014-06-16 [PMID: 24932685] (Human)
• Banerjee J, Das Ghatak P, Roy S, Khanna S, Sequin E, Bellman K, Dickinson B, Suri P, Subramaniam V, Chang C, Sen C Improvement of human keratinocyte migration by a redox active bioelectric dressing. PLoS ONE, 2014-03-03;9(3):e89239. 2014-03-03 [PMID: 24595050] (Human)
• Reinhard M, Frystyk J, Jespersen B, Bjerre M, Christiansen J, Flyvbjerg A, Ivarsen P Effect of hyperinsulinemia during hemodialysis on the insulin-like growth factor system and inflammatory biomarkers: a randomized open-label crossover study. BMC Nephrol, 2013-04-04;14(0):80. 2013-04-04 [PMID: 23557110] (Human)
• O'Brien NA, Browne BC, Chow L, Wang Y, Ginther C, Arboleda J, Duffy MJ, Crown J, O'Donovan N, Slamon DJ Activated phosphoinositide 3-kinase/AKT signaling confers resistance to trastuzumab but not lapatinib. Mol. Cancer Ther., 2010-05-25;9(6):1489-502. 2010-05-25 [PMID: 20501798] (Human)

Review for IGF-I R/IGF1R (DYC1770-2) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Patricia Carulla Martí 06/22/2023

Summary

• Sample Tested: Cell lysate from Human Keratinocytes (Hacat) in presence or absence of IGF,Cultured Human Keratinocytes
• Lot: P307442

FAQs for IGF-I R/IGF1R (DYC1770-2). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IGF1R