Human NT-3 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human NT-3 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: NTF3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human NT-3 DuoSet ELISA, 15 Plate

• HDNF
• MGC129711
• Nerve growth factor 2
• Neurotrophic factor
• neurotrophin 3
• neurotrophin-3
• NGF2
• NGF-2
• NT3
• NT-3
• NTF3

Background

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a noncovalently linked homodimer.

Human NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse, and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.

Publications for NT-3 (DY267)(16)

Publications using DY267

• Machairiotis, N;Vrachnis, D;Antonakopoulos, N;Loukas, N;Fotiou, A;Pergialiotis, V;Stavros, S;Mantzou, A;Maroudias, G;Iavazzo, C;Kanaka-Gantenbein, C;Drakakis, P;Troupis, T;Vlasis, K;Vrachnis, N; Detection and Quantification of Neurotrophin-3 (NT-3) and Nerve Growth Factor (NGF) Levels in Early Second Trimester Amniotic Fluid: Investigation into a Possible Correlation with Abnormal Fetal Growth Velocity Patterns Journal of clinical medicine 2023-06-19 [PMID: 37373824] (Human)
• HL Lee, CE Yeum, H Lee, J Oh, JT Kim, WJ Lee, Y Ha, YI Yang, KN Kim Peripheral Nerve-Derived Stem Cell Spheroids Induce Functional Recovery and Repair after Spinal Cord Injury in Rodents International Journal of Molecular Sciences, 2021-04-16;22(8):. 2021-04-16 [PMID: 33923671] (Human)
• KM Prautsch, A Schmidt, V Paradiso, DJ Schaefer, R Guzman, DF Kalbermatt, S Madduri Modulation of Human Adipose Stem Cells' Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro Cells, 2020-08-21;9(9):. 2020-08-21 [PMID: 32839392] (Human)
• Q Han, JD Ordaz, NK Liu, Z Richardson, W Wu, Y Xia, W Qu, Y Wang, H Dai, YP Zhang, CB Shields, GM Smith, XM Xu Descending motor circuitry required for NT-3 mediated locomotor recovery after spinal cord injury in mice Nat Commun, 2019-12-20;10(1):5815. 2019-12-20 [PMID: 31862889] (Human)
• H Li, Z Yang, W Wang, J Wang, J Zhang, J Liu, T Yang, Y Yang, J Wei, D Lei, X Yang NT-3/TrkC Axis Contributes to the Perineural Invasion and the Poor Prognosis in Human Salivary Adenoid Cystic Carcinoma J Cancer, 2019-10-15;10(24):6065-6073. 2019-10-15 [PMID: 31762816] (Human, Rat)
• U Milbreta, J Lin, C Pinese, W Ong, JS Chin, H Shirahama, R Mi, A Williams, ME Bechler, J Wang, C Ffrench-Co, A Hoke, SY Chew Scaffold-Mediated Sustained, Non-viral Delivery of miR-219/miR-338 Promotes CNS Remyelination Mol. Ther., 2018-12-01;0(0):. 2018-12-01 [PMID: 30611662]
• Y Zeng, H Han, B Tang, J Chen, D Mao, M Xiong Transplantation of Recombinant Vascular Endothelial Growth Factor (VEGF)189-Neural Stem Cells Downregulates Transient Receptor Potential Vanilloid 1 (TRPV1) and Improves Motor Outcome in Spinal Cord Injury Med. Sci. Monit., 2018-02-21;24(0):1089-1096. 2018-02-21 [PMID: 29466323] (Rat)
• Cynthia Azeredo Cordeiro Serum levels of neurotrophic factors in active toxoplasmic retinochoroiditis Braz J Infect Dis, 2016-12-05;0(0):. 2016-12-05 [PMID: 27932287] (Human)
• Dynamic Response Genes in CD4+ T Cells Reveal a Network of Interactive Proteins that Classifies Disease Activity in Multiple Sclerosis Cell Rep, 2016-09-13;16(11):2928-39. 2016-09-13 [PMID: 27626663] (Human)
• Yao , L, Daly , W, Newland , B, Yao , S, Wang , W, Chen , B K K, Madigan , N, Windebank , A, Pandit , A Improved axonal regeneration of transected spinal cord mediated by multichannel collagen conduits functionalized with neurotrophin-3 gene. Gene Ther, 2013-07-25;20(12):1149-57. 2013-07-25 [PMID: 23883961] (Human)
• Jones JL, Anderson JM, Phuah CL, Fox EJ, Selmaj K, Margolin D, Lake SL, Palmer J, Thompson SJ, Wilkins A, Webber DJ, Compston DA, Coles AJ Improvement in disability after alemtuzumab treatment of multiple sclerosis is associated with neuroprotective autoimmunity. Brain, 2010-07-21;133(0):2232-47. 2010-07-21 [PMID: 20659956] (Human)
• Saygili E, Schauerte P, Kuppers F, Heck L, Weis J, Weber C, Schwinger RH, Hoffmann R, Schroder JW, Marx N, Rana OR Electrical stimulation of sympathetic neurons induces autocrine/paracrine effects of NGF mediated by TrkA. J. Mol. Cell. Cardiol., 2010-02-02;49(1):79-87. 2010-02-02 [PMID: 20138055] (Rat)
• Mastroeni R, Bensadoun JC, Charvin D, Aebischer P, Pujol A, Raoul C Insulin-like growth factor-1 and neurotrophin-3 gene therapy prevents motor decline in an X-linked adrenoleukodystrophy mouse model. Ann. Neurol., 2009-07-01;66(1):117-22. 2009-07-01 [PMID: 19670442] (Human)
• Nelson PG, Kuddo T, Song EY Selected neurotrophins, neuropeptides, and cytokines: developmental trajectory and concentrations in neonatal blood of children with autism or Down syndrome. Int. J. Dev. Neurosci., 2005-11-14;24(1):73-80. 2005-11-14 [PMID: 16289943] (Human)
• Caggiula M, Batocchi AP, Frisullo G, Angelucci F, Patanella AK, Sancricca C, Nociti V, Tonali PA, Mirabella M Neurotrophic factors in relapsing remitting and secondary progressive multiple sclerosis patients during interferon beta therapy. Clin. Immunol., 2005-11-07;118(1):77-82. 2005-11-07 [PMID: 16275091] (Human)
• Taylor SJ, McDonald JW, Sakiyama-Elbert SE Controlled release of neurotrophin-3 from fibrin gels for spinal cord injury. J Control Release, 2004-08-11;98(2):281-94. 2004-08-11 [PMID: 15262419] (Human)

FAQs for NT-3 (DY267). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NTF3