Human/Mouse Cleaved Caspase-3 (Asp175) DuoSet IC, 2 Plate



Product Details

Reactivity Hu, MuSpecies Glossary
Applications ELISA

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Human/Mouse Cleaved Caspase-3 (Asp175) DuoSet IC, 2 Plate Summary

Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details


Application Notes
No significant interference observed with available related molecules.
Read Publications using DYC835-2.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human/Mouse Cleaved Caspase-3 (Asp175) DuoSet IC, 2 Plate

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA


Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. They are expressed as latent zymogens and are activated by an autoproteolytic mechanism or by processing by other proteases (frequently other caspases). Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4, -5, and -13), apoptosis initiation (caspase-2, -8, -9, -and -10), and apoptosis execution (caspase-3, -6, and -7).

Caspases are regulated by a variety of stimili, including APAF1, CFLAR/FLIP, NOL3/ARC, and members of the inhibitor of apoptosis (IAP) family such as BIRC1/NAIP, BIRC2/cIAP-1, BIRC3/cIAP-2, BIRC4/XIAP, BIRC5/Survivin, and BIRC7/Livin. IAP activity is modulated by DIABLO/SMAC or PRSS25/HTRA2/Omi. Cell-permeable and irreversible peptide inhibitors are also available for different caspases.

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Publications for Caspase-3 (DYC835-2)(19)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Complex Species Category.

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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using DYC835-2 Applications Species
ZH Al-Oanzi, FO Alenazy, HH Alhassan, MR El-Aassar, AI Alzarea, SI Alzarea, AM Abbas, MH Alanazi, MM Al-Enazi Effects of Thymoquinone Alone or in Combination with Losartan on the Cardiotoxicity Caused by Oxidative Stress and Inflammation in Hypercholesterolemia Journal of cardiovascular development and disease, 2022;9(12):. 2022 [PMID: 36547425] (Rat) Rat
Bhuyan S, Pal B, Pathak L, Saikia P, Mitra S, Gayan S, Mokhtari R, Li H, Ramana C, Baishya D, Das B Targeting hypoxia-induced tumor stemness by activating pathogen-induced stem cell niche defense. Front Immunol, 0;13(0):933329. 0 [PMID: 36248858] (Complex Species Category) Complex Species Category
N Fukunaga, RVP Ribeiro, V Bissoondat, F Billia, V Rao Ghrelin May Inhibit Inflammatory Response and Apoptosis During Ischemia-Reperfusion Injury Transplantation Proceedings, 2022;0(0):. 2022 [PMID: 36180256] (Mouse) Mouse
F Aybar, M Julia Pere, M Silvina Ma, M Eugenia Sa, M Marrodan, J María Pasq, J Correale 2-Chlorodeoxyadenosine (Cladribine) preferentially inhibits the biological activity of microglial cells International immunopharmacology, 2022;105(0):108571. 2022 [PMID: 35093689] (Mouse) Mouse
Y Jung, M Son, YR Nam, J Choi, JR Heath, S Yang Microfluidic Single-Cell Proteomics Assay Chip: Lung Cancer Cell Line Case Study Micromachines, 2021;12(10):. 2021 [PMID: 34683198] (Human) Human
K Liu, J Liu, B Zou, C Li, HJ Zeh, R Kang, G Kroemer, J Huang, D Tang Trypsin-mediated sensitization to ferroptosis increases the severity of pancreatitis in mice Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):. 2021 [PMID: 34562639] (Mouse) Mouse
CY Ko, PC Shih, PW Huang, YH Lee, YF Chen, MH Tai, CH Liu, ZH Wen, HM Kuo Sinularin, an Anti-Cancer Agent Causing Mitochondria-Modulated Apoptosis and Cytoskeleton Disruption in Human Hepatocellular Carcinoma International Journal of Molecular Sciences, 2021;22(8):. 2021 [PMID: 33920454] (Human) Human
S Medina, X Zhou, FT Lauer, H Zhang, KJ Liu, J Lewis, SW Burchiel Modulation of PARP activity by Monomethylarsonous (MMA+3) acid and uranium in mouse thymus Toxicology and Applied Pharmacology, 2020;411(0):115362. 2020 [PMID: 33279514] (Mouse) Mouse
NM El Bakary, AZ Alsharkawy, ZA Shouaib, EMS Barakat Role of Bee Venom and Melittin on Restraining Angiogenesis and Metastasis in gamma-Irradiated Solid Ehrlich Carcinoma-Bearing Mice Integr Cancer Ther, 2020;19(0):1534735420944. 2020 [PMID: 32735464] (Mouse) Mouse
AH Shen, MR Borrelli, S Adem, NMD Deleon, RA Patel, S Mascharak, SJ Yen, BY Sun, WL Taylor, M Januszyk, DH Nguyen, A Momeni, GC Gurtner, MT Longaker, DC Wan Prophylactic treatment with transdermal deferoxamine mitigates radiation-induced skin fibrosis Sci Rep, 2020;10(1):12346. 2020 [PMID: 32704071] (Mouse) Mouse
Show All 19 Publications.

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Blogs on Caspase-3. Showing 1-10 of 11 blog posts - Show all blog posts.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

COVID-19 and metabolic dysregulation: SARS-CoV-2 injures human exocrine and endocrine pancreas
Jamshed Arslan, Pharm D, PhD Humans rely on the pancreas for digesting food and generating energy from it. SARS-CoV-2-mediated damage to the exocrine pancreas is evident from the pancreatitis, pancreatic enlargeme...  Read full blog post.

  Read full blog post.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of ...  Read full blog post.

Cleaved Caspase-3: A Marker of Programmed Cell Death
What are Caspases?Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environme...  Read full blog post.

Caspase-3, The Executioner of Apoptosis
The Role of Caspase-3 in ApoptosisCaspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinat...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

D4-GDI (GDP dissociation inhibitor, RhoGD12)
The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.

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Gene Symbol CASP3