Human Mesothelin DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Mesothelin DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: MSLN

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Mesothelin DuoSet ELISA, 15 Plate

• CAK1 antigen
• CAK1
• megakaryocyte potentiating factor
• Mesothelin
• MPF
• MPFSMRP
• MSLN
• Pre-pro-megakaryocyte-potentiating factor
• SMR
• soluble MPF mesothelin related protein

Background

Mesothelin, also known as CAK1 and ERC, is derived from a precursor that also includes Megakaryocyte Potentiating Factor (MPF). Following cleavage of the precursor, Mesothelin remains attached to the cell surface via a GPI linkage. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. It is upregulated in mesotheliomas and a range of carcinomas and adenomas. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression.

Publications for Mesothelin (DY3265)(3)

Publications using DY3265

• C Jiménez-Ra, D Gilbert We, G Aguilar-Ma, A Brik, CA Juárez-Pér, S Casjens, I Raiko, T Brüning, G Johnen, A Cabello-Ló Assessment of miR-103a-3p in leukocytes-No diagnostic benefit in combination with the blood-based biomarkers mesothelin and calretinin for malignant pleural mesothelioma diagnosis PLoS ONE, 2022-10-14;17(10):e0275936. 2022-10-14 [PMID: 36240245] (Human)
• G Aguilar-Ma, B Pesch, ES Calderón-A, K Burek, C Jiménez-Ra, CA Juárez-Pér, MD Ochoa-Vázq, L Torre-Bous, LC Acosta-Saa, I Sada-Ovall, J García-Fig, I Alvarado-C, P Castillo-G, AR Báez-Salda, JR Pérez-Padi, J Osnaya-Juá, RM Rivera-Ros, EM García-Baz, YL Bautista-A, E Lazcano-He, DA Munguía-Ca, LM Argote-Gre, D Taeger, DG Weber, S Casjens, I Raiko, T Brüning, G Johnen Biomarkers for Predicting Malignant Pleural Mesothelioma in a Mexican Population Int J Med Sci, 2018-06-04;15(9):883-891. 2018-06-04 [PMID: 30008600] (Human)
• Kagedan D, Lecker I, Batruch I Characterization of the seminal plasma proteome in men with prostatitis by mass spectrometry. Clin Proteomics, 2012-02-06;9(1):2. 2012-02-06 [PMID: 22309592] (Human)

FAQs for Mesothelin (DY3265). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: MSLN