Human KGF/FGF-7 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human KGF/FGF-7 Quantikine ELISA Kit Summary

• Background: The Quantikine Human KGF Immunoassay is a 5.25 or 5.5 hour solid phase ELISA designed to measure KGF in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human KGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant KGF accurately. Results obtained measuring natural human KGF showed d...ose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human KGF.
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• Specificity: Natural and recombinant human KGF
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: FGF7

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human KGF/FGF-7 Quantikine ELISA Kit

• FGF7
• FGF-7
• fibroblast growth factor 7HBGF-7
• HBGF7
• HBGF-7
• Heparin-binding growth factor 7
• keratinocyte growth factor
• KGF
• KGFfibroblast growth factor 7 (keratinocyte growth factor)

Background

KGF (keratinocyte growth factor), also known as FGF-7, is induced in mesenchymal cells by inflammation or tissue damage. It functions as a paracrine factor during wound healing by inducing the proliferation of nearby epithelial cells. KGF plays a role in kidney morphogenesis, hair follicle development, and keratinocyte differentiation. It signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (IIIb)/KGF R.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for KGF/FGF-7 (DKG00)(15)

Publications using DKG00

• E Prieto-Gar, CV Díaz-Garcí, A Agudo-Lópe, V Pardo-Marq, I García-Con, S Asensio-Pe, M Alonso-Ria, C Pérez, C Gómez, J Adeva, L Paz-Ares, JA López-Mart, MT Agulló-Ort Tumor-Stromal Interactions in a Co-Culture Model of Human Pancreatic Adenocarcinoma Cells and Fibroblasts and Their Connection with Tumor Spread Biomedicines, 2021-03-31;9(4):. 2021-03-31 [PMID: 33807441] (Human)
• SM Nam, YS Maeng, EK Kim, KY Seo, H Lew Ex Vivo Expansion of Human Limbal Epithelial Cells Using Human Placenta-Derived and Umbilical Cord-Derived Mesenchymal Stem Cells Stem Cells Int, 2017-08-15;2017(0):4206187. 2017-08-15 [PMID: 28894469] (Human)
• Impact of Hyperglycemia and Low Oxygen Tension on Adipose-Derived Stem Cells Compared with Dermal Fibroblasts and Keratinocytes: Importance for Wound Healing in Type 2 Diabetes PLoS ONE, 2016-12-19;11(12):e0168058. 2016-12-19 [PMID: 27992567] (Human)
• Saito S, Morishima K, Ui T, Hoshino H, Matsubara D, Ishikawa S, Aburatani H, Fukayama M, Hosoya Y, Sata N, Lefor A, Yasuda Y, Niki T The role of HGF/MET and FGF/FGFR in fibroblast-derived growth stimulation and lapatinib-resistance of esophageal squamous cell carcinoma. BMC Cancer, 2015-02-25;15(0):82. 2015-02-25 [PMID: 25884729] (Human)
• Zheng C, Cotrim AP, Sunshine AN, Sugito T, Liu L, Sowers A, Mitchell JB, Baum BJ Prevention of radiation-induced oral mucositis after adenoviral vector-mediated transfer of the keratinocyte growth factor cDNA to mouse submandibular glands. Clin. Cancer Res., 2009-07-07;15(14):4641-8. 2009-07-07 [PMID: 19584147] (Mouse)
• Nafidi O, Lapointe RW, Lepage R, Kumar R, D'Amour P Mechanisms of renal phosphate loss in liver resection-associated hypophosphatemia. Ann. Surg., 2009-05-01;249(5):824-7. 2009-05-01 [PMID: 19387319] (Human)
• Chandel NS, Budinger GR, Mutlu GM, Varga J, Synenki L, Donnelly HK, Zirk A, Eisenbart J, Jovanovic B, Jain M Keratinocyte growth factor expression is suppressed in early acute lung injury/acute respiratory distress syndrome by smad and c-Abl pathways. Crit. Care Med., 2009-05-01;37(5):1678-84. 2009-05-01 [PMID: 19325470] (Human)
• Kellouche S, Martin C, Korb G, Rezzonico R, Bouard D, Benbunan M, Dubertret L, Soler C, Legrand C, Dosquet C Tissue engineering for full-thickness burns: a dermal substitute from bench to bedside. Biochem. Biophys. Res. Commun., 2007-09-04;363(3):472-8. 2007-09-04 [PMID: 17888881] (Human)
• Yashiro M, Nakazawa K, Tendo M, Kosaka K, Shinto O, Hirakawa K Selective cyclooxygenase-2 inhibitor downregulates the paracrine epithelial-mesenchymal interactions of growth in scirrhous gastric carcinoma. Int. J. Cancer, 2007-02-01;120(3):686-93. 2007-02-01 [PMID: 17096355] (Human)
• Groschl M, Topf HG, Kratzsch J, Dotsch J, Rascher W, Rauh M Salivary leptin induces increased expression of growth factors in oral keratinocytes. J. Mol. Endocrinol., 2005-04-01;34(2):353-66. 2005-04-01 [PMID: 15821102] (Human)
• Witte RP, Kao WJ Keratinocyte-fibroblast paracrine interaction: the effects of substrate and culture condition. Biomaterials, 2005-06-01;26(17):3673-82. 2005-06-01 [PMID: 15621258] (Human)
• Park JU, Tsuchiya T Increase in gap junctional intercellular communication by high molecular weight hyaluronic acid associated with fibroblast growth factor 2 and keratinocyte growth factor production in normal human dermal fibroblasts. Tissue Eng., 2002-07-01;8(3):419-27. 2002-07-01 [PMID: 12167228] (Human)
• Danan C, Franco ML, Jarreau PH, Dassieu G, Chailley-Heu B, Bourbon J, Delacourt C High concentrations of keratinocyte growth factor in airways of premature infants predicted absence of bronchopulmonary dysplasia. Am. J. Respir. Crit. Care Med., 2002-05-15;165(10):1384-7. 2002-05-15 [PMID: 12016100] (Human)
• McCabe AJ, Carlino U, Holm BA, Glick PL Upregulation of keratinocyte growth factor in the tracheal ligation lamb model of congenital diaphragmatic hernia. J. Pediatr. Surg., 2001-01-01;36(1):128-32. 2001-01-01 [PMID: 11150451] (Ovine)
• Szabowski A, Maas-Szabowski N, Andrecht S, Kolbus A, Schorpp-Kistner M, Fusenig NE, Angel P c-Jun and JunB antagonistically control cytokine-regulated mesenchymal-epidermal interaction in skin. Cell, 2000-11-22;103(5):745-55. 2000-11-22 [PMID: 11114331] (Human)

FAQs for KGF/FGF-7 (DKG00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: FGF7