Human IL-34 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-34 Quantikine ELISA Kit Summary

• Background: The Quantikine Human IL-34 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-34 in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human IL-34 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-34. Results obtained using natural human IL-...34 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-34.
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• Specificity: Natural and recombinant human IL-34
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL34

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-34 Quantikine ELISA Kit

• C16orf77
• IL34
• IL-34
• interleukin 34

Background

Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers. It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level.

IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen . The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R). IL-34 stimulates monocyte proliferation. In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes. In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-34 (D3400)(4)

Publications using D3400

• N Hagan, JL Kane, D Grover, L Woodworth, C Madore, J Saleh, J Sancho, J Liu, Y Li, J Proto, M Zelic, A Mahan, M Kothe, AA Scholte, M Fitzgerald, B Gisevius, A Haghikia, O Butovsky, D Ofengeim CSF1R signaling is a regulator of pathogenesis in progressive MS Cell Death Dis, 2020-10-23;11(10):904. 2020-10-23 [PMID: 33097690] (Human)
• S Mathews, A Branch Woo, I Katano, E Makarov, MB Thomas, HE Gendelman, LY Poluektova, M Ito, S Gorantla Human Interleukin-34 facilitates microglia-like cell differentiation and persistent HIV-1 infection in humanized mice Mol Neurodegener, 2019-03-05;14(1):12. 2019-03-05 [PMID: 30832693] (Humanized Mouse)
• R Tao, Q Fan, H Zhang, H Xie, L Lu, G Gu, F Wang, R Xi, J Hu, Q Chen, W Niu, W Shen, R Zhang, X Yan Prognostic Significance of Interleukin-34 (IL-34) in Patients With Chronic Heart Failure With or Without Renal Insufficiency J Am Heart Assoc, 2017-04-01;6(4):. 2017-04-01 [PMID: 28365566] (Human)
• IL-34 is associated with the presence and severity of renal dysfunction and coronary artery disease in patients with heart failure Sci Rep, 2016-12-16;6(0):39324. 2016-12-16 [PMID: 27982136] (Human)

FAQs for IL-34 (D3400). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: IL34