Human IL-16 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-16 Quantikine ELISA Kit Summary

• Background: The Quantikine Human IL-16 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IL-16 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-16 and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human IL-16. Results obtained using natural IL-16 showed linear curves t...hat were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-16.
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• Specificity: Natural and recombinant human IL-16
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL16

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-16 Quantikine ELISA Kit

• FLJ16806
• FLJ42735
• FLJ44234
• HsT19289
• IL16
• IL-16
• interleukin 16 (lymphocyte chemoattractant factor)
• LCF
• LCFprIL-16
• lymphocyte chemoattractant factor
• neuronal interleukin 16
• NIL16
• PRIL16
• prointerleukin 16
• pro-interleukin-16

Background

Interleukin 16 (IL-16), also named lymphocyte chemoattractant factor (LCF), is a 14-17 kDa single chain non-glycosylated polypeptide that was originally identified as a CD8+ T cell-derived chemoattractant for CD4+ cells. The expression of IL-16 precursor mRNA has been detected in various tissues including spleen, thymus, lymph nodes, peripheral leukocytes, bone marrow and cerebellum. In addition to its chemotactic properties, IL-16 has also been shown to suppress HIV-1 replication in vitro.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-16 (D1600)(13)

Publications using D1600

• D Reinhold, V Farztdinov, Y Yan, C Meisel, H Sadlowski, J Kühn, FH Perschel, M Endres, E Düzel, S Vielhaber, K Guttek, A Goihl, M Venø, B Teegen, W Stöcker, P Stubbemann, F Kurth, LE Sander, M Ralser, C Otto, S Streit, S Jarius, K Ruprecht, H Radbruch, J Kjems, M Mülleder, F Heppner, P Körtvelyes The brain reacting to COVID-19: analysis of the cerebrospinal fluid proteome, RNA and inflammation Journal of Neuroinflammation, 2023-02-09;20(1):30. 2023-02-09 [PMID: 36759861] (Human)
• O Kofanova, E Henry, RA Quesada, A Bulla, HN Linares, P Lescuyer, K Shea, M Stone, G Tybring, C Bellora, F Betsou IL8 and IL16 levels indicate serum and plasma quality Clin. Chem. Lab. Med., 2018-06-27;0(0):. 2018-06-27 [PMID: 29425105] (Human)
• Y Zhang, LT Fu, F Tang The protective effects of magnolol on acute trinitrobenzene sulfonic acid?induced colitis in rats Mol Med Rep, 2017-12-20;0(0):. 2017-12-20 [PMID: 29286109] (Rat)
• E Das Gupta, WR Ng, SF Wong, AK Bhurhanude, SS Yeap Correlation of serum cartilage oligometric matrix protein (COMP) and interleukin-16 (IL-16) levels with disease severity in primary knee osteoarthritis: A pilot study in a Malaysian population PLoS ONE, 2017-09-14;12(9):e0184802. 2017-09-14 [PMID: 28910372] (Human)
• Vendrame F, Cataldo D, Ciarlo L In type 1 diabetes immunocompetent cells are defective in IL-16 secretion. Scand. J. Immunol., 2012-01-01;75(1):127-8. 2012-01-01 [PMID: 21916924] (Human)
• Ghigo E, Barry AO, Pretat L, Al Moussawi K, Desnues B, Capo C, Kornfeld H, Mege JL IL-16 promotes T. whipplei replication by inhibiting phagosome conversion and modulating macrophage activation. PLoS ONE, 2010-10-21;5(10):e13561. 2010-10-21 [PMID: 21042409] (Human)
• Tsai IS, Tsai CC, Ho YP, Ho KY, Wu YM, Hung CC Interleukin-12 and interleukin-16 in periodontal disease. Cytokine, 2005-07-07;31(1):34-40. 2005-07-07 [PMID: 15886011] (Human)
• Qin XJ, Shi HZ, Huang ZX, Kang LF, Mo WN, Wu C Interleukin-16 in tuberculous and malignant pleural effusions. Eur. Respir. J., 2005-04-01;25(4):605-11. 2005-04-01 [PMID: 15802332] (Human)
• Barak V, Elchalal U, Edelstein M, Kalickman I, Lewin A, Abramov Y Interleukin-18 levels correlate with severe ovarian hyperstimulation syndrome. Fertil. Steril., 2004-08-01;82(2):415-20. 2004-08-01 [PMID: 15302292] (Human)
• Alexandrakis MG, Passam FH, Kyriakou DS, Christophoridou AV, Perisinakis K, Hatzivasili A, Foudoulakis A, Castanas E Serum level of interleukin-16 in multiple myeloma patients and its relationship to disease activity. Am. J. Hematol., 2004-02-01;75(2):101-6. 2004-02-01 [PMID: 14755377] (Human)
• Burton CT, »V6þÌ™+ìZ, Hardy GA, Sullivan AK, Nelson MR, Gazzard B, Gotch FM, Imami N Impact of NNRTI compared to PI-based highly active antiretroviral therapy on CCR5 receptor expression, beta-chemokines and IL-16 secretion in HIV-1 infection. 2002-11-01;130(2):286-92. 2002-11-01 [PMID: 12390317] (Human)
• Binninger-Schinzel D, 2019, Norley S, Adler HS, Oberg HH, Kurth R Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes. 2002-03-01;294(1):209-21. 2002-03-01 [PMID: 11886279] (Human)
• Cotler SJ, Reddy KR, McCone J, Wolfe DL, Liu A, Craft TR, Ferris MW, Conrad AJ, Albrecht J, Morrissey M, Ganger DR, Rosenblate H, Blatt LM, Jensen DM, Taylor MW An analysis of acute changes in interleukin-6 levels after treatment of hepatitis C with consensus interferon. J. Interferon Cytokine Res., 2001-12-01;21(12):1011-9. 2001-12-01 [PMID: 11798458] (Human)

FAQs for IL-16 (D1600). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL16