Human IL-12 p70 Quantikine HS ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-12 p70 Quantikine HS ELISA Kit Summary

• Background: The Quantikine HS Human IL-12 Immunoassay is a 6.5 hour solid phase ELISA designed to measure IL-12 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human IL-12 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-12 accurately. Results obtained using natural human IL-12 showed ...linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-12.
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• Specificity: Natural and recombinant human IL-12p70
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL12A

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-12 p70 Quantikine HS ELISA Kit

• CLMF p35
• Cytotoxic lymphocyte maturation factor 35 kDa subunit
• IL12 p70
• IL-12 p70
• IL-12 subunit p35
• IL-12, subunit p35
• interleukin 12, p35
• interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
• interleukin-12 alpha chain
• interleukin-12 subunit alpha
• natural killer cell stimulatory factor 1, 35 kD subunit
• NF cell stimulatory factor chain 1
• NK cell stimulatory factor chain 1
• NKSF1
• p35

Background

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Publications for IL-12 p70/IL-12A (HS120)(9)

Publications using HS120

• Koutaki, D;Paltoglou, G;Manou, M;Vourdoumpa, A;Ramouzi, E;Tzounakou, AM;Michos, A;Bacopoulou, F;Mantzou, E;Zoumakis, E;Papadopoulou, M;Kassari, P;Charmandari, E; The Role of Secreted Frizzled-Related Protein 5 (Sfrp5) in Overweight and Obesity in Childhood and Adolescence Nutrients 2024-09-17 [PMID: 39339733] (Human)
• Neubauer-Geryk, J;Wielicka, M;My?liwiec, M;Zorena, K;Bieniaszewski, L; The Impact of Metabolic Memory on Immune Profile in Young Patients with Uncomplicated Type 1 Diabetes International journal of molecular sciences 2024-03-10 [PMID: 38542165] (Human)
• Z Yang, V Pietrobon, M Bobbin, O Stefanson, J Yang, A Goswami, B Alphson, H Choi, K Magallanes, Q Cai, D Barrett, B Wang, LS Qi, FM Marincola Nanoscale, antigen encounter-dependent, IL-12 delivery by CAR T cells plus PD-L1 blockade for cancer treatment Journal of Translational Medicine, 2023-02-28;21(1):158. 2023-02-28 [PMID: 36855120] (Human)
• N Fuke, K Aizawa, H Suganuma, T Takagi, Y Naito Effect of combined consumption of Lactobacillus brevis KB290 and ?-carotene on minor diarrhoea-predominant irritable bowel syndrome-like symptoms in healthy subjects: a randomised, double-blind, placebo-controlled, parallel-group trial Int J Food Sci Nutr, 2017-04-09;0(0):1-14. 2017-04-09 [PMID: 28391736] (Human)
• B Kowalewska, K Zorena, M Szmigiero-, P W??, M My?liwiec High Interleukin-12 Levels May Prevent an Increase in the Amount of Fungi in the Gastrointestinal Tract during the First Years of Diabetes Mellitus Type 1 Dis. Markers, 2016-12-29;2016(0):4685976. 2016-12-29 [PMID: 28127111] (Human)
• Gluzman-Poltorak Z, Mendonca S, Vainstein V, Kha H, Basile L Randomized comparison of single dose of recombinant human IL-12 versus placebo for restoration of hematopoiesis and improved survival in rhesus monkeys exposed to lethal radiation. J Hematol Oncol, 2014-04-06;7(0):31. 2014-04-06 [PMID: 24708888] (Human, Primate - Macaca mulatta (Rhesus Macaque))
• Chapgier A, Wynn RF, Jouanguy E, Filipe-Santos O, Zhang S, Feinberg J, Hawkins K, Casanova JL, Arkwright PD Human complete Stat-1 deficiency is associated with defective type I and II IFN responses in vitro but immunity to some low virulence viruses in vivo. J. Immunol., 2006-04-15;176(8):5078-83. 2006-04-15 [PMID: 16585605] (Human)
• Yamanaka K, Clark R, Dowgiert R, Hurwitz D, Shibata M, Rich BE, Hirahara K, Jones DA, Eapen S, Mizutani H, Kupper TS Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma. Clin. Cancer Res., 2006-01-15;12(2):376-82. 2006-01-15 [PMID: 16428475] (Human)
• Bartosik-Psujek H, Stelmasiak Z Correlations between IL-4, IL-12 levels and CCL2, CCL5 levels in serum and cerebrospinal fluid of multiple sclerosis patients. J Neural Transm, 2004-10-12;112(6):797-803. 2004-10-12 [PMID: 15480850] (Human)

Bioinformatics

• Gene Symbol: IL12A